Genetic expression of Menkes disease in cultured astrocytes of the macular mouse

1991 ◽  
Vol 14 (6) ◽  
pp. 896-901 ◽  
Author(s):  
H. Kodama ◽  
Y. Meguro ◽  
T. Abe ◽  
M. H. Rayner ◽  
K. T. Suzuki ◽  
...  
Keyword(s):  
Menkes Disease ◽  
Genetic Expression ◽  
Cultured Astrocytes ◽  
Macular Mouse

scholarly journals Occurrence of two missense mutations in Cu-ATPase of the macular mouse, a menkes disease model

IUBMB Life ◽  
1997 ◽  
Vol 43 (4) ◽  
pp. 913-918 ◽  
Author(s):  
Yuriko Ohta ◽  
Noriyuki Shiraishi ◽  
Morimitsu Nishikimi
Keyword(s):  
Disease Model ◽  
Menkes Disease ◽  
Missense Mutations ◽  
Macular Mouse

Effect of copper and disulfiram combination therapy on the macular mouse, a model of Menkes disease

2012 ◽  
Vol 26 (2-3) ◽  
pp. 105-108 ◽  
Author(s):  
Wattanaporn Bhadhprasit ◽  
Hiroko Kodama ◽  
Chie Fujisawa ◽  
Tomoko Hiroki ◽  
Eishin Ogawa
Keyword(s):  
Combination Therapy ◽  
Menkes Disease ◽  
Macular Mouse ◽  
Effect Of Copper

scholarly journals Menkes disease: Oral administration of glyoxal-bis(N(4)-methylthiosemicarbazonato)-copper(II) rescues the macular mouse

2018 ◽  
Vol 84 (5) ◽  
pp. 770-777 ◽  
Author(s):  
Mitsutoshi Munakata ◽  
Hiroko Kodama ◽  
Norihiko Tani ◽  
Kazuhiko Kimura ◽  
Hideyo Takahashi ◽  
...  
Keyword(s):  
Oral Administration ◽  
Menkes Disease ◽  
Macular Mouse

Mottled gene expression and copper distribution in the macular mouse, an animal model for Menkes disease

1998 ◽  
Vol 21 (3) ◽  
pp. 199-202 ◽  
Author(s):  
Y. Murata ◽  
H. Kodama ◽  
Y. Mori ◽  
M. Kobayashi ◽  
T. Abe
Keyword(s):  
Gene Expression ◽  
Animal Model ◽  
Menkes Disease ◽  
Macular Mouse ◽  
Copper Distribution

scholarly journals Mutation Analysis and Expression of the Mottled Gene in the Macular Mouse Model of Menkes Disease

1997 ◽  
Vol 42 (4) ◽  
pp. 436-442 ◽  
Author(s):  
Yoshiko Murata ◽  
Hiroko Kodama ◽  
Toshiaki Abe ◽  
Norio Ishida ◽  
Masahiko Nishimura ◽  
...  
Keyword(s):  
Mouse Model ◽  
Mutation Analysis ◽  
Menkes Disease ◽  
Macular Mouse

scholarly journals Copper-trafficking efficacy of copper-pyruvaldehyde bis(N4-methylthiosemicarbazone) on the macular mouse, an animal model of Menkes disease

2012 ◽  
Vol 72 (3) ◽  
pp. 270-276 ◽  
Author(s):  
Mitsutoshi Munakata ◽  
Hiroko Kodama ◽  
Chie Fujisawa ◽  
Tomoko Hiroki ◽  
Kazuhiko Kimura ◽  
...  
Keyword(s):  
Animal Model ◽  
Menkes Disease ◽  
Copper Trafficking ◽  
Macular Mouse

Effect of copper and diethyldithiocarbamate combination therapy on the macular mouse, an animal model of Menkes disease

2005 ◽  
Vol 28 (6) ◽  
pp. 971-978 ◽  
Author(s):  
H. Kodama ◽  
E. Sato ◽  
Y.-H. Gu ◽  
K. Shiga ◽  
C. Fujisawa ◽  
...  
Keyword(s):  
Animal Model ◽  
Combination Therapy ◽  
Menkes Disease ◽  
Macular Mouse ◽  
Effect Of Copper

Preparation of cultured astrocytes for x-ray microanalysis

1989 ◽  
Vol 47 ◽  
pp. 988-989
Author(s):  
N.K.R. Smith ◽  
K.E. Hunter ◽  
P. Mobley ◽  
L.P. Felpel
Keyword(s):  
Cultured Cells ◽  
Elemental Content ◽  
Elemental Distribution ◽  
Sprague Dawley ◽  
X Ray ◽  
Cultured Astrocytes ◽  
Freeze Dried ◽  
Ultimate Objective

Electron probe energy dispersive x-ray microanalysis (XRMA) offers a powerful tool for the determination of intracellular elemental content of biological tissue. However, preparation of the tissue specimen , particularly excitable central nervous system (CNS) tissue , for XRMA is rather difficult, as dissection of a sample from the intact organism frequently results in artefacts in elemental distribution. To circumvent the problems inherent in the in vivo preparation, we turned to an in vitro preparation of astrocytes grown in tissue culture. However, preparations of in vitro samples offer a new and unique set of problems. Generally, cultured cells, growing in monolayer, must be harvested by either mechanical or enzymatic procedures, resulting in variable degrees of damage to the cells and compromised intracel1ular elemental distribution. The ultimate objective is to process and analyze unperturbed cells. With the objective of sparing others from some of the same efforts, we are reporting the considerable difficulties we have encountered in attempting to prepare astrocytes for XRMA.Tissue cultures of astrocytes from newborn C57 mice or Sprague Dawley rats were prepared and cultured by standard techniques, usually in T25 flasks, except as noted differently on Cytodex beads or on gelatin. After different preparative procedures, all samples were frozen on brass pins in liquid propane, stored in liquid nitrogen, cryosectioned (0.1 μm), freeze dried, and microanalyzed as previously reported.

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