The isolation of adult rat liver parenchymal cells

1985 ◽  
Vol 9 (2) ◽  
pp. 49-52 ◽  
Author(s):  
Joan A. McGowan ◽  
Nancy L. R. Bucher
Keyword(s):  
Rat Liver ◽  
Liver Parenchymal ◽  
Adult Rat ◽  
Parenchymal Cells

scholarly journals Induction of amino acid transport in primary cultures of adult rat liver parenchymal cells by insulin.

1976 ◽  
Vol 251 (10) ◽  
pp. 3014-3020 ◽  
Author(s):  
R F Kletzien ◽  
M W Pariza ◽  
J E Becker ◽  
V R Potter ◽  
F R Butcher
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Amino Acid Transport ◽  
Primary Cultures ◽  
Acid Transport ◽  
Liver Parenchymal ◽  
Adult Rat ◽  
Parenchymal Cells

Effect of dexamethasone on the isozyme pattern of adult rat liver parenchymal cells in primary cultures

1982 ◽  
Vol 45 (2) ◽  
Author(s):  
JuanEmilio Fel�u ◽  
Julio Coloma ◽  
Mar�a-Jos� G�mez-Lech�n ◽  
Mar�aDolores Garc�a ◽  
Jos� B�guena
Keyword(s):  
Rat Liver ◽  
Primary Cultures ◽  
Isozyme Pattern ◽  
Liver Parenchymal ◽  
Adult Rat ◽  
Parenchymal Cells

scholarly journals Regulation of coenzyme A biosynthesis by glucagon and glucocorticoid in adult rat liver parenchymal cells

1980 ◽  
Vol 188 (1) ◽  
pp. 175-184 ◽  
Author(s):  
Colleen M. Smith ◽  
C. Richard Savage
Keyword(s):  
Cyclic Amp ◽  
Rat Liver ◽  
Maximum Effect ◽  
Maximal Effect ◽  
Dibutyryl Cyclic Amp ◽  
Liver Parenchymal ◽  
Adult Rat ◽  
Intracellular Mechanism ◽  
Inhibitor Of Protein Synthesis ◽  
Parenchymal Cells

We studied the effects of glucagon, dibutyryl cyclic AMP and dexamethasone on the rate of [14C]pantothenate conversion to CoA in adult rat liver parenchymal cells in primary culture. The presence of 30nm-glucagon increased the rate by about 1.5-fold relative to control cultures (range 1.4–2.3) and 2.4-fold relative to cultures containing 1–3m-i.u. of insulin/ml. The half-maximal effect was obtained at 3nm-glucagon. Dibutyryl cyclic AMP plus theophylline also enhanced the rate by about 1.5-fold. Dexamethasone acted synergistically with glucagon; glucagon at 0.3nm had no effect when added alone, but resulted in a 1.7-fold enhancement when added in the presence of dexamethasone (maximum effect at 50nm). The 1.4-fold enhancement caused by the addition of saturating glucagon concentrations was increased to a 3-fold overall enhancement by the addition of dexamethasone. However, dexamethasone added alone over the range 5nm to 5μm had no effect on the rate of [14C]pantothenate conversion to CoA. The stimulatory effect of dibutyryl cyclic AMP plus theophylline was also enhanced by the addition of dexamethasone. Changes in intracellular pantothenate concentration or radioactivity could not account for the stimulatory effects of glucagon, dibutyryl cyclic AMP or dexamethasone. Addition of 18μm-cycloheximide, an inhibitor of protein synthesis, decreased the rate of incorporation of [14C]pantothenate into CoA and the enhancement of this rate by glucagon and dibutyryl cyclic AMP plus theophylline in a reversible manner. These results demonstrate an influence of glucagon, dibutyryl cyclic AMP and glucocorticoids on the intracellular mechanism regulating total CoA concentrations in the liver.

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