Membrane characteristics of adult rat liver parenchymal cells in primary monolayer culture

In Vitro ◽  
1980 ◽  
Vol 16 (10) ◽  
pp. 833-846 ◽  
Author(s):  
A. L. Tarentino ◽  
J. Galivan
Keyword(s):  
Rat Liver ◽  
Monolayer Culture ◽  
Liver Parenchymal ◽  
Adult Rat ◽  
Primary Monolayer Culture ◽  
Primary Monolayer ◽  
Parenchymal Cells

scholarly journals PARENCHYMAL CELLS FROM ADULT RAT LIVER IN NONPROLIFERATING MONOLAYER CULTURE

1973 ◽  
Vol 59 (3) ◽  
pp. 722-734 ◽  
Author(s):  
D. Montgomery Bissell ◽  
Lydia E. Hammaker ◽  
Urs A. Meyer
Keyword(s):  
Rat Liver ◽  
Monolayer Culture ◽  
Glycogen Synthesis ◽  
Adult Rat ◽  
Metabolic Functions ◽  
Primary Monolayer ◽  
Collagenase Perfusion ◽  
Parenchymal Cells ◽  
Plastic Tissue Culture

Parenchymal cells from adult rat liver have been established in primary monolayer culture. Donor animals are subjected to a partial hepatectomy and, 4 days later, cells are prepared by collagenase perfusion of the regenerated liver. The hepatic parenchymal cells, separated from nonparenchymal material and suspended in serum-free medium, are placed in plastic tissue culture dishes, where they form a monolayer within 24 h. The monolayer cells exhibit minimal mitotic activity and demonstrate several major metabolic functions characteristic of liver in vivo; these include albumin synthesis and secretion, gluconeogenesis from 3-carbon precursors, responsiveness to insulin and glucagon, glycogen synthesis, and activity of two microsomal enzymes. These functions are present in the monolayer cells for several days at activities similar to those observed in the liver in vivo. The findings indicate that hepatic parenchymal cells in this monolayer system are viable and behave in many respects like normal adult rat liver.

scholarly journals Induction of amino acid transport in primary cultures of adult rat liver parenchymal cells by insulin.

1976 ◽  
Vol 251 (10) ◽  
pp. 3014-3020 ◽  
Author(s):  
R F Kletzien ◽  
M W Pariza ◽  
J E Becker ◽  
V R Potter ◽  
F R Butcher
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Amino Acid Transport ◽  
Primary Cultures ◽  
Acid Transport ◽  
Liver Parenchymal ◽  
Adult Rat ◽  
Parenchymal Cells

scholarly journals The effect of insulin-dextran complexes on the protein synthesis in the primary monolayer culture of adult rat hepatocytes.

1984 ◽  
Vol 144 (3) ◽  
pp. 245-256
Author(s):  
TSUGUHIKO NAKAI ◽  
YASUNORI KUTSUMI ◽  
YOSHIKAZU SAKAMOTO ◽  
KOJI OIDA ◽  
SUSUMU MIYABO ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Monolayer Culture ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Primary Monolayer Culture ◽  
Adult Rat Hepatocytes ◽  
Primary Monolayer
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