Endogenous interferon α/β produced by Kupffer cells inhibits interleukin-1, tumor necrosis factor α production and interleukin-2-induced activation of nonparenchymal liver cells

1991 ◽  
Vol 34 (3) ◽  
pp. 150-156 ◽  
Author(s):  
Shie-Pon Tzung ◽  
Stefan A. Cohen
Keyword(s):  
Tumor Necrosis Factor ◽  
Kupffer Cells ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Interleukin 1 ◽  
Interleukin 2 ◽  
Liver Cells ◽  
Interferon Α ◽  
Factor Α ◽  
Necrosis Factor

Verotoxin 1 from Escherichia coli Affects Gb3/CD77+ Bovine Lymphocytes Independent of Interleukin-2, Tumor Necrosis Factor-α, and Interferon-α

2003 ◽  
Vol 228 (4) ◽  
pp. 377-386 ◽  
Author(s):  
Christian Menge ◽  
Ivonne Stamm ◽  
Maike Blessenohl ◽  
Lothar H. Wieler ◽  
Georg Baljer
Keyword(s):  
Escherichia Coli ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Interleukin 2 ◽  
Γδ T Cells ◽  
Interferon Α ◽  
Bovine Lymphocytes ◽  
Factor Α ◽  
Necrosis Factor

Verotoxin (VT)-induced immunomodulation has been implicated in the ability of VT-producing Escherichia coli (VTEC) to cause persistent infections in cattle. VT1, also referred to as Shiga toxin 1, is a potent cytotoxin that modulates cytokine secretions and functions. This prompted the current investigation to examine whether the inhibiting effect of VT1 on bovine lymphocytes correlates with the expression of the cellular VT1 receptor Gb3/CD77 or is mediated instead via perturbation of cytokine secretion. Using blood mononuclear cells stimulated by mitogens as a model, VT1 significantly blocked lymphoblast transformation and proliferation in the BoCD8+ T cell and BoCD21+ B cell population. In contrast, VT1 dramatically reduced the number of viable Gb3/CDTZ+ blast cells within all subpopulations identified (BoCD2+, BoCD4+, BoCD8+, WC1+ [i.e., γδ T cells] BoCD21+, and BoCD25+). Similar effects of VT1 were observed when the culture medium was supplemented with selected cytokines: tumor necrosis factor-α-sensitizing endothelial cells against VT1, interferon-a (IFN-α) as bovine IFN-α receptors are partially homologous to the B-subunit of VT1, and interleukin-2 that is critical for lymphocyte proliferation in vitro. The addition of these cytokines was neither able to mimic nor to overcome the effects of VT1. Therefore, it is concluded that VT1 directly acts on bovine lymphocytes rather than inducing a cytokine-mediated effect. VT1 considerably affects all main bovine lymphocyte subpopulations, implicating that the immune system is a predominant target for VT1 In cattle.

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