A one-step efficient and specific non-radioactive non-fluorescent method for in situ hybridization of banded chromosomes

Chromosoma ◽  
1990 ◽  
Vol 99 (6) ◽  
pp. 436-439 ◽  
Author(s):  
F. R. Zhang ◽  
R. Heilig ◽  
G. Thomas ◽  
A. Aurias
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent Method ◽  
One Step

In situ hybridization of CoOX nanoparticles on N-doped graphene through one step mineralization of co-responsive hydrogels

2017 ◽  
Vol 46 (19) ◽  
pp. 6163-6167 ◽  
Author(s):  
Jing Ma ◽  
Xiaojuan Wang ◽  
Ting He ◽  
Minli Tan ◽  
Jun Zheng ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Electrocatalytic Properties ◽  
Doped Graphene ◽  
One Step ◽  
Responsive Hydrogels

Involving GO into Co-PT hydrogels can enhance the strength of hydrogels. The electrocatalytic properties of CoOX/N-rGO700 aerogels are improved by annealing.

scholarly journals Hybridization chain reaction enables a unified approach to multiplexed, quantitative, high-resolution immunohistochemistry and in situ hybridization

Development ◽  
2021 ◽  
Vol 148 (22) ◽  
Author(s):  
Maayan Schwarzkopf ◽  
Mike C. Liu ◽  
Samuel J. Schulte ◽  
Rachel Ives ◽  
Naeem Husain ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
High Resolution ◽  
Signal Amplification ◽  
Brain Slices ◽  
Hybridization Chain Reaction ◽  
Unified Framework ◽  
Chain Reaction ◽  
One Step ◽  
Rna Imaging

ABSTRACT RNA in situ hybridization based on the mechanism of the hybridization chain reaction (HCR) enables multiplexed, quantitative, high-resolution RNA imaging in highly autofluorescent samples, including whole-mount vertebrate embryos, thick brain slices and formalin-fixed paraffin-embedded tissue sections. Here, we extend the benefits of one-step, multiplexed, quantitative, isothermal, enzyme-free HCR signal amplification to immunohistochemistry, enabling accurate and precise protein relative quantitation with subcellular resolution in an anatomical context. Moreover, we provide a unified framework for simultaneous quantitative protein and RNA imaging with one-step HCR signal amplification performed for all target proteins and RNAs simultaneously.

scholarly journals In situ hybridization and cytochemistry: localization of mRNA at stained neuromuscular junctions with 33P-labeled probes.

1994 ◽  
Vol 42 (10) ◽  
pp. 1407-1411 ◽  
Author(s):  
E Liu ◽  
M M Salpeter
Keyword(s):  
In Situ Hybridization ◽  
Ache Activity ◽  
Neuromuscular Junctions ◽  
Original Method ◽  
Sternocleidomastoid Muscle ◽  
Staining Reaction ◽  
Epsilon Subunit ◽  
Subunit Mrna ◽  
One Step

We modified the Karnovsky and Roots method of staining sites of acetylcholinesterase (AChE) activity at neuromuscular junctions (NMJs) to survive the lengthy, multiple steps of in situ hybridization and autoradiography. When the original method of Karnovsky and Roots is used to identify the muscle endplates, the stain does not survive the in situ hybridization procedures and association of mRNA to specific endplates can be inferred only indirectly. The successful modification involves secondary staining with diaminobenzidine (DAB) and H2O2 using the Karnovsky-Roots staining reaction product as a catalyst. Mounted longitudinal cryosections of mouse sternocleidomastoid muscle were fixed and stained in one step on the slide with paraformaldehyde plus the Karnovsky-Roots stain, followed by DAB-H2O2 secondary staining. The tissues were then processed for in situ hybridization and probed for the acetylcholine receptor (AChR) epsilon-subunit mRNA, known to be localized at the NMJ. The probe was labeled with 33P, which is ideal for in situ hybridization. By this procedure, the endplate stain was retained even after the hybridization and autoradiographic procedures, and the developed grains due to radiolabeling of the AChR epsilon-subunit mRNA were localized at readily identified endplates.

scholarly journals A Novel Fluorescent Method for in situ Hybridization.

1993 ◽  
Vol 26 (5) ◽  
pp. 441-445 ◽  
Author(s):  
NAOTO KAGIYAMA ◽  
KIYOHITO YOSHIDA ◽  
TAKASHI HAMABATA ◽  
NAOTO JUNI ◽  
TAKESHI AWASAKI ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent Method

DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

1992 ◽  
Vol 50 (1) ◽  
pp. 496-497
Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Dual Band ◽  
Nick Translation ◽  
Metaphase Chromosomes ◽  
Band Pass ◽  
Texas Red ◽  
Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

Ultrastructural analysis of the spatial distribution of MRNA

1992 ◽  
Vol 50 (1) ◽  
pp. 552-553
Author(s):  
Gary Bassell ◽  
Robert H. Singer
Keyword(s):  
Electron Microscopy ◽  
Spatial Distribution ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Nucleic Acids ◽  
Colloidal Gold ◽  
Dna Probe ◽  
Nucleotide Analogs ◽  
Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

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