scholarly journals Development of a Novel One-Step Automated Rapid in situ Hybridization for Anaplastic Lymphoma Kinase Rearrangement Using Non-Contact Alternating-Current Electric-Field Mixing

Pathobiology ◽  
2020 ◽  
Vol 87 (1) ◽  
pp. 45-50
Author(s):  
Kazuhiro Imai ◽  
Shinogu Takashima ◽  
Satoshi Fujishima ◽  
Tsubasa Matsuo ◽  
Shin-nosuke Watanabe ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Electric Field ◽  
Anaplastic Lymphoma Kinase ◽  
Alternating Current ◽  
Anaplastic Lymphoma ◽  
Anaplastic Lymphoma Kinase Rearrangement ◽  
One Step ◽  
Current Electric Field

scholarly journals Novel method for rapid in-situ hybridization of HER2 using non-contact alternating-current electric-field mixing

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Yoshitaro Saito ◽  
Kazuhiro Imai ◽  
Ryuta Nakamura ◽  
Hiroshi Nanjo ◽  
Kaori Terata ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Electric Field ◽  
Alternating Current ◽  
Novel Method ◽  
Current Electric Field

scholarly journals The Use of Three-Dimensional DNA Fluorescent In Situ Hybridization (3D DNA FISH) for the Detection of Anaplastic Lymphoma Kinase (ALK) in Non-Small Cell Lung Cancer (NSCLC) Circulating Tumor Cells

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1465 ◽  
Author(s):  
Arutha Kulasinghe ◽  
Yenkai Lim ◽  
Joanna Kapeleris ◽  
Majid Warkiani ◽  
Ken O’Byrne ◽  
...  
Keyword(s):  
Lung Cancer ◽  
In Situ Hybridization ◽  
Tumor Cells ◽  
Anaplastic Lymphoma Kinase ◽  
Fluorescent In Situ Hybridization ◽  
Small Cell ◽  
Small Cell Lung ◽  
Anaplastic Lymphoma ◽  
Nsclc Patients

Tumor tissue biopsy is often limited for non-small cell lung cancer (NSCLC) patients and alternative sources of tumoral information are desirable to determine molecular alterations such as anaplastic lymphoma kinase (ALK) rearrangements. Circulating tumor cells (CTCs) are an appealing component of liquid biopsies, which can be sampled serially over the course of treatment. In this study, we enrolled a cohort of ALK-positive (n = 8) and ALK-negative (n = 12) NSCLC patients, enriched for CTCs using spiral microfluidic technology and performed DNA fluorescent in situ hybridization (FISH) for ALK. CTCs were identified in 12/20 NSCLC patients ranging from 1 to 26 CTCs/7.5 mL blood. Our study revealed that 3D imaging of CTCs for ALK translocations captured a well-defined separation of 3′ and 5′ signals indicative of ALK translocations and overlapping 3′/5′ signal was easily resolved by imaging through the nuclear volume. This study provides proof-of-principle for the use of 3D DNA FISH in the determination of CTC ALK translocations in NSCLC.

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