Improved diagnosis of cowpea aphid-borne mosaic virus in Africa: significance for cowpea seed-indexing, breeding programs and potyvirus taxonomy

1996 ◽  
Vol 141 (1) ◽  
pp. 137-145 ◽  
Author(s):  
C. Huguenot ◽  
M. T. Furneaux ◽  
J. A. Clare ◽  
R. I. Hamilton
Keyword(s):  
Mosaic Virus ◽  
Breeding Programs ◽  
Cowpea Aphid ◽  
Cowpea Seed

scholarly journals ​​Screening Cowpea Genotypes for Resistance to Cowpea Aphid Borne Mosaic Virus (CABMV) and Cowpea Severe Mosaic Virus (CPSMV) in Paraguay

2021 ◽  
Author(s):  
Guillermo Alonso ◽  
Pablo Duarte ◽  
Viviana M. Camelo-García ◽  
Arnaldo Esquivel-Fariña ◽  
Elliot W. Kitajima ◽  
...  
Keyword(s):  
Virus Infection ◽  
Mosaic Virus ◽  
Rural Families ◽  
Virus Infections ◽  
Carbohydrate Source ◽  
Breeding Programs ◽  
Cowpea Cultivars ◽  
Cowpea Aphid ◽  
Resistant Genotypes ◽  
Elisa Result

Background: Cowpea [Vigna unguiculata (L) Walp.] is one of the Paraguayan rural families’ main crops, serving as an essential protein and carbohydrate source. Cowpea aphid borne mosaic virus (CABMV) and cowpea severe mosaic virus (CPSMV) were identified infecting cowpea plants. Disease control caused by both viruses is difficult because there is no information about local cowpea resistant cultivars and vector control is not practical. Methods: In the present work, sixteen cowpea genotypes/cultivars were mechanically inoculated with local isolates of CABMV and CPSMV to identify resistant genotypes/cultivars that can be used in breeding programs. Virus infections were determined by symptoms expression and confirmed by PTA-ELISA. Result: Genotypes Arroz rojo (V. angularis), TVu 379, TVu 382, TE94-256-2E and TE97-309G-9 were resistant to CABMV. Genotypes Arroz rojo (V. angularis), CNCX-698-128F, TVu 379, TVu 382, TVu-3961, TE97-309G-9 and TE97-309G-3 were resistant to CPSMV. Overall, this study showed that local cowpea cultivars do not offer any resistance to virus infection and the need for resistant germplasms for cowpea breeding programs in the country.

scholarly journals Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea

2018 ◽  
Vol 40 (1) ◽  
Author(s):  
NAYARA NIELE SACOMAN ◽  
ALEXANDRE PIO VIANA ◽  
VIRGINIA SILVA CARVALHO ◽  
EILEEN AZEVEDO SANTOS ◽  
ROSANA RODRIGUES
Keyword(s):  
Mosaic Virus ◽  
Elisa Test ◽  
Passion Fruit ◽  
The Other ◽  
Visual Evaluation ◽  
Breeding Programs ◽  
Genetic Breeding ◽  
Fruit Species ◽  
Cowpea Aphid

ABSTRACT Passion fruit (Passiflora edulis) is a fruit species of great relevance for Brazilian economy. However, it is highly susceptible to the Cowpea aphid-borne mosaic virus (CABMV). The species P.setacea, on the other hand, is resistant to the disease. The present study aimed to identify CABMV-resistant P. setacea genotypes for the introgression of genes into sour passion fruit genetic breeding programs. The seeds of passion fruit genotypes were germinated in vitro in MS culture medium. The seedlings were acclimatized in a growth chamber at the temperature of 27 ± 2ºC, photoperiod of 16:8 hours and 80% of relative humidity. Thirty plants of each genotype were mechanically inoculated with extract prepared from leaves collected from passion fruit plants with symptoms of CABMV for the assessment of resistance to CABMV. The severity of leaf symptoms was evaluated by means of a grading scale of visual signs. After the visual evaluation and identification of the asymptomatic genotypes of P. setacea, the PTA-ELISA test was carried out for 30 selected genotypes. According to the visual evaluation, all P. setacea genotypes were highly resistant to CABMV, while the P. edulis genotypes were highly susceptible. Out of the 30 genotypes selected, only PsRJ 4 was considered susceptible by PTA-ELISA. The other genotypes of P. setacea were considered resistant and present great potential for use in passion fruit genetic breeding programs.

Exploring New Sources of Viral Resistance in Cowpea Germplasm and Its Elaboration through Multivariate Analysis

2021 ◽  
Author(s):  
M.S. Iqbal ◽  
S.A. Hussain ◽  
N. Arshad ◽  
S. Munir ◽  
M.A. Ali ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Leaf Shape ◽  
Bean Common Mosaic Virus ◽  
Viral Resistance ◽  
Plant Type ◽  
Cowpea Aphid ◽  
Two Factors ◽  
Eigen Values ◽  
Virus Strains

Background: Cowpea is a major food legume rich in protein but its production has been dwindling by several factors including viral infection due to various virus strains in all agro-ecological zones.Methods: Sixty eight cowpea genotypes were screened against qualitative traits (leaf shape, seed surface, twinning tendency, anthocynin pigment, plant type, fodder type and cream color) and four seed borne viruses viz. cucumber mosaic virus (CMV), cowpea aphid borne mosaic virus (CABMV), black eye cowpea mosaic virus (BlCMV) and bean common mosaic virus (BCMV) under both in situ and laboratory conditions using DAC-ELISA.Result: Based on in situ screening, 16 genotypes were found resistant to all the four viruses, whereas for ELISA, 13 genotypes (27005, 27041, 27075, 27141, 27145, 27146, 27147, 27158, 27160, 27167, 27172, IT85F-1380 and IT86D-719) were found resistant to all. Twelve clusters were obtained from UPGMA based on disease severity. Genotype 27008 (Pakistan) was present in cluster VI and was susceptible to all antisera CMV, CABMV, BICMV and BCMV. Whereas 13 genotypes were present in cluster VIII which were found resistant to all the four antisera applied. Therefore, 13 genotypes suggested for safe use in any breeding program at developing resistant cultivars. First two factors obtained through PCA with eigen-values greater than 1 contributed greater than 80 per cent variability. Twelve distinct groups were observed and these were in coordination with cluster analysis.

scholarly journals Yellow passion fruit reaction to Xanthomonas axonopodis pv. passiflorae and to Cowpea aphid-borne mosaic virus

2018 ◽  
Vol 18 (4) ◽  
pp. 349-356
Author(s):  
Anne Pinheiro Costa ◽  
Isadora Nogueira ◽  
José Ricardo Peixoto ◽  
Michelle de Souza Vilela ◽  
Luiz Eduardo Bassay Blum ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Passion Fruit ◽  
Xanthomonas Axonopodis ◽  
Cowpea Aphid ◽  
Yellow Passion Fruit
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