Analysis of CD34-positive hemopoietic progenitor cells from normal human adult peripheral blood: flow-cytometrical studies and in-vitro colony (CFU-GM, BFU-E) assays

1991 ◽  
Vol 62 (2-3) ◽  
pp. 45-53 ◽  
Author(s):  
Stefan Serke ◽  
Sabine Säsuberlich ◽  
Yoshikazu Abe ◽  
Dieter Huhn
Keyword(s):  
Blood Flow ◽  
Progenitor Cells ◽  
Peripheral Blood ◽  
Peripheral Blood Flow ◽  
Human Adult ◽  
Normal Human ◽  
Adult Peripheral Blood ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells

scholarly journals Myeloperoxidase expression in CD34+ normal human hematopoietic cells

Blood ◽  
1993 ◽  
Vol 82 (7) ◽  
pp. 2069-2078 ◽  
Author(s):  
H Strobl ◽  
M Takimoto ◽  
O Majdic ◽  
G Fritsch ◽  
C Scheinecker ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Transferrin Receptor ◽  
Staining Pattern ◽  
Hematopoietic Progenitor ◽  
Colony Forming Unit ◽  
Cd34 Cells ◽  
Normal Human ◽  
Colony Forming Capacity ◽  
Adult Peripheral Blood

Abstract Bone marrow (BM), adult peripheral blood (aPB), and umbilical cord blood (CB) samples contain small proportions of CD34+ cells that include virtually all hematopoietic progenitor cells. Myeloperoxidase (MPO) is considered to be selectively expressed in cells committed to granulomonocytic differentiation. Using flow cytometry and an antibody against MPO, we studied at which stage of normal hematopoietic differentiation CD34+ cells being to express MPO. We consistently observed a characteristic MPO/CD34 staining pattern and found that 35% +/- 9% of CD34+ BM cells express MPO. The MPO+ CD34+ subset and the CD33+ CD34+ subset were of similar size and overlapped considerably. MPO+ CD34+ cells expressed high levels of HLA-D molecules, were weakly CD71/transferrin receptor positive to negative, were CD45RA+ and lacked the CD45RO isoform of the leukocyte common antigen. Additionally, MPO+ CD34+ cells were on average larger in size than MPO- CD34+ cells. Virtually identical phenotypic features have previously been described for in vitro colony-forming granulomonocytic progenitor cells. In vitro clonogenic assays performed with MPO-enriched and MPO-depleted fractions of CD34+ BM cells performed by us also suggest, but do not formally prove, that at least a portion of MPO+ CD34+ cells have in vitro cluster (10 to 50 cells/colony) or colony-forming unit granulocyte-macrophage (> or = 50 cells/colony) forming capacity. CD34+ cells from CB and aPB resembled CD34+ BM cells in that considerable proportions of them coexpressed CD33. However, in contrast to BM, CD34+ cells from CB and aPB samples lacked significant MPO expression and, in line with this, the majority of them (CB, 59% +/- 7%; aPB, 66% +/- 5%) coexpressed CD45RO.

Characterization of CD34+ Human Hemopoietic Progenitor Cells from the Peripheral Blood: Enzyme-, Carbohydrate- and Immunocytochemistry, Morphometry, and Ultrastructure

1995 ◽  
Vol 16 (5-6) ◽  
pp. 483-492 ◽  
Author(s):  
Juergen Thiele ◽  
Claudia Wickenhauser ◽  
Stephan E. Baldus ◽  
Thomas Kuemmel ◽  
Thomas K. Zirbes ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Peripheral Blood ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells

CD34+ Human Hemopoietic Progenitor Cells of the Bone Marrow Differ from Those of the Peripheral Blood: An Immunocytochemical and Morphometric Study

1995 ◽  
Vol 93 (2-4) ◽  
pp. 83-90 ◽  
Author(s):  
Claudia Wickenhauser ◽  
Jürgen Thiele ◽  
Uta Drebber ◽  
Hans Michael Kvasnicka ◽  
Andreas Thiel ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Peripheral Blood ◽  
Morphometric Study ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells

scholarly journals Myeloperoxidase expression in CD34+ normal human hematopoietic cells

Blood ◽  
1993 ◽  
Vol 82 (7) ◽  
pp. 2069-2078 ◽  
Author(s):  
H Strobl ◽  
M Takimoto ◽  
O Majdic ◽  
G Fritsch ◽  
C Scheinecker ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Transferrin Receptor ◽  
Staining Pattern ◽  
Hematopoietic Progenitor ◽  
Colony Forming Unit ◽  
Cd34 Cells ◽  
Normal Human ◽  
Colony Forming Capacity ◽  
Adult Peripheral Blood

Bone marrow (BM), adult peripheral blood (aPB), and umbilical cord blood (CB) samples contain small proportions of CD34+ cells that include virtually all hematopoietic progenitor cells. Myeloperoxidase (MPO) is considered to be selectively expressed in cells committed to granulomonocytic differentiation. Using flow cytometry and an antibody against MPO, we studied at which stage of normal hematopoietic differentiation CD34+ cells being to express MPO. We consistently observed a characteristic MPO/CD34 staining pattern and found that 35% +/- 9% of CD34+ BM cells express MPO. The MPO+ CD34+ subset and the CD33+ CD34+ subset were of similar size and overlapped considerably. MPO+ CD34+ cells expressed high levels of HLA-D molecules, were weakly CD71/transferrin receptor positive to negative, were CD45RA+ and lacked the CD45RO isoform of the leukocyte common antigen. Additionally, MPO+ CD34+ cells were on average larger in size than MPO- CD34+ cells. Virtually identical phenotypic features have previously been described for in vitro colony-forming granulomonocytic progenitor cells. In vitro clonogenic assays performed with MPO-enriched and MPO-depleted fractions of CD34+ BM cells performed by us also suggest, but do not formally prove, that at least a portion of MPO+ CD34+ cells have in vitro cluster (10 to 50 cells/colony) or colony-forming unit granulocyte-macrophage (> or = 50 cells/colony) forming capacity. CD34+ cells from CB and aPB resembled CD34+ BM cells in that considerable proportions of them coexpressed CD33. However, in contrast to BM, CD34+ cells from CB and aPB samples lacked significant MPO expression and, in line with this, the majority of them (CB, 59% +/- 7%; aPB, 66% +/- 5%) coexpressed CD45RO.

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