Effects of recombinant human tumor necrosis factor (rhtnf) on normal human and mouse hemopoietic progenitor cells

1987 ◽  
Vol 5 (1) ◽  
pp. 16-26 ◽  
Author(s):  
Kouichi Akahane ◽  
Takayuki Hosoi ◽  
Akio Urabe ◽  
Fumimaro Takaku ◽  
Masanobu Kawakami
Keyword(s):  
Tumor Necrosis Factor ◽  
Progenitor Cells ◽  
Tumor Necrosis ◽  
Human Tumor ◽  
Human Tumor Necrosis Factor ◽  
Normal Human ◽  
Human And Mouse ◽  
Necrosis Factor ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells

scholarly journals Effect of recombinant human tumor necrosis factor on the colony growth of human leukemia progenitor cells and normal hematopoietic progenitor cells

Blood ◽  
1987 ◽  
Vol 69 (2) ◽  
pp. 467-472 ◽  
Author(s):  
T Murase ◽  
T Hotta ◽  
H Saito ◽  
R Ohno
Keyword(s):  
Tumor Necrosis Factor ◽  
Progenitor Cells ◽  
Tumor Necrosis ◽  
Human Tumor ◽  
Colony Growth ◽  
Inhibitory Effect ◽  
Myelogenous Leukemia ◽  
Human Leukemia ◽  
Human Tumor Necrosis Factor ◽  
Necrosis Factor

The effects of recombinant human tumor necrosis factor (rH-TNF) on the colony growth of human leukemia progenitor cells (L-CFU), granulocyte- macrophage progenitor cells (CFU-GM), and erythroid progenitor cells (BFU-E) were studied. L-CFU was assayed with leukemia cells obtained from patients with acute myelogenous leukemia. CFU-GM and BFU-E were assayed with bone marrow cells obtained from hematologically normal donors and patients with acute leukemia or non-Hodgkin's lymphoma in complete remission. A dose-dependent growth inhibition of L-CFU as well as CFU-GM and BFU-E was observed by rH-TNF at concentrations of 1 to 100 U/mL. The inhibitory effect on L-CFU was significantly greater than that on CFU-GM. No correlation was observed between the inhibitory effect on L-CFU and the number of colonies formed in the cultures without rH-TNF. Preincubation of the progenitor cells in culture medium containing 20% fetal calf serum with up to 1,000 U/mL of rH-TNF for 24 hours did not result in the inhibition of colony growth of L-CFU or CFU- GM. The inhibitory effect of rH-TNF was neutralized by an anti-rH-TNF murine monoclonal antibody.

scholarly journals Effect of recombinant human tumor necrosis factor on the colony growth of human leukemia progenitor cells and normal hematopoietic progenitor cells

Blood ◽  
1987 ◽  
Vol 69 (2) ◽  
pp. 467-472 ◽  
Author(s):  
T Murase ◽  
T Hotta ◽  
H Saito ◽  
R Ohno
Keyword(s):  
Tumor Necrosis Factor ◽  
Progenitor Cells ◽  
Tumor Necrosis ◽  
Human Tumor ◽  
Colony Growth ◽  
Inhibitory Effect ◽  
Myelogenous Leukemia ◽  
Human Leukemia ◽  
Human Tumor Necrosis Factor ◽  
Necrosis Factor

Abstract The effects of recombinant human tumor necrosis factor (rH-TNF) on the colony growth of human leukemia progenitor cells (L-CFU), granulocyte- macrophage progenitor cells (CFU-GM), and erythroid progenitor cells (BFU-E) were studied. L-CFU was assayed with leukemia cells obtained from patients with acute myelogenous leukemia. CFU-GM and BFU-E were assayed with bone marrow cells obtained from hematologically normal donors and patients with acute leukemia or non-Hodgkin's lymphoma in complete remission. A dose-dependent growth inhibition of L-CFU as well as CFU-GM and BFU-E was observed by rH-TNF at concentrations of 1 to 100 U/mL. The inhibitory effect on L-CFU was significantly greater than that on CFU-GM. No correlation was observed between the inhibitory effect on L-CFU and the number of colonies formed in the cultures without rH-TNF. Preincubation of the progenitor cells in culture medium containing 20% fetal calf serum with up to 1,000 U/mL of rH-TNF for 24 hours did not result in the inhibition of colony growth of L-CFU or CFU- GM. The inhibitory effect of rH-TNF was neutralized by an anti-rH-TNF murine monoclonal antibody.

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