Comparative evaluation of two commercial assays for direct detection ofMycobacterium tuberculosis in respiratory specimens

1998 ◽  
Vol 17 (3) ◽  
pp. 151-157
Author(s):  
F. Gamboa ◽  
J. M. Manterola ◽  
J. Lonca ◽  
L. Matas ◽  
P. J. Cardona ◽  
...  
Keyword(s):  
Comparative Evaluation ◽  
Direct Detection ◽  
Ofmycobacterium Tuberculosis ◽  
Detection Ofmycobacterium Tuberculosis ◽  
Respiratory Specimens

scholarly journals Comparison of Enhanced Mycobacterium tuberculosisAmplified Direct Test with COBAS AMPLICOR Mycobacterium tuberculosis Assay for Direct Detection ofMycobacterium tuberculosis Complex in Respiratory and Extrapulmonary Specimens

2000 ◽  
Vol 38 (4) ◽  
pp. 1559-1562 ◽  
Author(s):  
Claudio Scarparo ◽  
Paola Piccoli ◽  
Alessandra Rigon ◽  
Giuliana Ruggiero ◽  
Mariuccia Scagnelli ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Direct Detection ◽  
Diagnostic Technique ◽  
Clinical Samples ◽  
Direct Test ◽  
Predictive Values ◽  
The Difference ◽  
Sensitivity Specificity ◽  
Detection Ofmycobacterium Tuberculosis ◽  
Respiratory Specimens

The new Roche COBAS AMPLICOR Mycobacterium tuberculosisAssay was compared to the Gen-Probe enhanced Mycobacterium tuberculosis Amplified Direct Test (AMTDII). A total of 486 specimens (296 respiratory and 190 extrapulmonary) collected from 323 patients were tested in parallel with both assays. Results were compared with those of acid-fast staining and culture, setting the combination of culture and clinical diagnosis as the “gold standard.” After resolution of discrepant results, the sensitivity, specificity, and positive and negative predictive values for AMTDII were 85.7, 100, 100, and 90.4% for respiratory specimens and 82.9, 100, 100, and 95.5% for extrapulmonary specimens, respectively. The corresponding values for AMPLICOR were 94.2, 100, 100, and 96.6% for respiratory specimens and 85, 100, 100, and 96.1% for extrapulmonary specimens, respectively. No significant differences were observed between the results of both assays or, within each one, between respiratory and extrapulmonary specimens. The difference between AMTDII and AMPLICOR sensitivities was related to the presence of inhibitory samples, which the former assay, lacking an internal amplification control (IAC), could not detect. The overall inhibition rate for the AMPLICOR assay was 3.9% (19 specimens). It is concluded that, although both amplification assays proved to be rapid and specific for the detection of M. tuberculosis complex in clinical samples, AMPLICOR, by a completely automated amplification and detection procedure, was shown to be particularly feasible for a routine laboratory setting. Finally, AMTDII is potentially an excellent diagnostic technique for both respiratory and extrapulmonary specimens, provided that an IAC is included with the assay.

Evaluation of two commercial amplification assays for detection ofMycobacterium tuberculosis complex in respiratory specimens

Infection ◽  
1995 ◽  
Vol 23 (4) ◽  
pp. 216-221 ◽  
Author(s):  
Manca Žolnir-Dovč ◽  
Alenka Rus ◽  
M. Poljak ◽  
Katja Seme ◽  
Tatjana Avšič-Županc
Keyword(s):  
Tuberculosis Complex ◽  
Ofmycobacterium Tuberculosis ◽  
Detection Ofmycobacterium Tuberculosis ◽  
Respiratory Specimens
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