Transient expression of a lysine-rich vicilin gene ofVicia faba in barley endosperm detected by immunological tissue printing after particle bombardment

1995 ◽  
Vol 15 (1-2) ◽  
pp. 125-128
Author(s):  
Ute Heim ◽  
Renate Manteuffel ◽  
Helmut Bäumlein ◽  
Hans-Henning Steinbiss ◽  
Ulrich Wobus
Keyword(s):  
Transient Expression ◽  
Particle Bombardment ◽  
Barley Endosperm ◽  
Tissue Printing ◽  
Ofvicia Faba ◽  
Vicilin Gene

Transient Expression of β-Glucuronidase in Embryo Axes of Cotton by Agrobacterium and Particle Bombardment Methods

2002 ◽  
Vol 45 (3) ◽  
pp. 359-365 ◽  
Author(s):  
A.K. Banerjee ◽  
D.C. Agrawal ◽  
S.M. Nalawade ◽  
K.V. Krishnamurthy
Keyword(s):  
Transient Expression ◽  
Particle Bombardment ◽  
Embryo Axes

Transformation of the developing barley endosperm by particle bombardment

Planta ◽  
1991 ◽  
Vol 185 (3) ◽  
Author(s):  
S�ren Knudsen ◽  
Martin M�ller
Keyword(s):  
Particle Bombardment ◽  
Barley Endosperm

scholarly journals Transient expression of DNA and RNA in parasitic helminths by using particle bombardment

1999 ◽  
Vol 96 (15) ◽  
pp. 8687-8692 ◽  
Author(s):  
R. E. Davis ◽  
A. Parra ◽  
P. T. LoVerde ◽  
E. Ribeiro ◽  
G. Glorioso ◽  
...  
Keyword(s):  
Transient Expression ◽  
Particle Bombardment ◽  
Parasitic Helminths ◽  
Dna And Rna

scholarly journals Cloning of SjCA gene and its expression analysis on upland cottons

2016 ◽  
Vol 2 (2) ◽  
pp. 150
Author(s):  
Jingjing Kong ◽  
Xuke Lu ◽  
Xiaojie Zhao ◽  
Zujun Yin ◽  
Shuai Wang ◽  
...  
Keyword(s):  
Transient Expression ◽  
Particle Bombardment ◽  
Expression System ◽  
Salt Resistance ◽  
Complete Sequence ◽  
Sequence Information ◽  
Gene Gun ◽  
Salt Tolerant ◽  
Germination Ability

Acquisition of salt-tolerant genes from exogenous plants to improve cotton salt resistance has always been a hotspot of research on cotton salt resistance. However, the information regarding the method of conversion of living cotton pollens by portable gene gun technology is still little. Complete sequence information of the Carbonic Anhydrase (CA) gene was obtained from NCBI database, and its full ORF length sequence was cloned by RT-PCR technology. After the construction of pBI121-CA::GFP fusion expression vector, we used cotton pollens from upland cotton varieties Y-2067, ZA-23, and GZ-2, which have weaker autofluorescence, to conduct the research on the transient expression of cotton pollens in vivo via particle bombardment (gene gun technology). The results indicated that green fluorescence enhancement of the three kinds of cotton pollens was realized after the CA gene transformation, meaning that the CA gene expression level was increased. Besides, the salt tolerance germination ability of transgenic T1 seeds was also improved. Our research initially established a transient expression system of cotton pollen in vivo via particle bombardment technology, laying the theoretical foundation for further research of cotton genetic transformation and creation of cotton salt-tolerant germplasm.

Particle bombardment-mediated transient expression of a Brazil nut methionine-rich albumin in bean (Phaseolus vulgaris L.)

1992 ◽  
Vol 20 (2) ◽  
pp. 357-359 ◽  
Author(s):  
Francisco J. L. Aragao ◽  
Maria F. Grossi de Sa ◽  
Eleonora R. Almeida ◽  
Eugen S. Gander ◽  
Elibio L. Rech
Keyword(s):  
Phaseolus Vulgaris ◽  
Transient Expression ◽  
Particle Bombardment ◽  
Brazil Nut ◽  
Phaseolus Vulgaris L
Sign in / Sign up

Export Citation Format

Share Document