Sensitization of gas-phase protein sequencer using fluorescein isothiocyanate FITC

1992 ◽  
Vol 11 (4) ◽  
pp. 359-359 ◽  
Author(s):  
Koji Muramoto ◽  
Kiyoshi Nokihara ◽  
Akira Ueda ◽  
Hisao Kamiya
Keyword(s):  
Gas Phase ◽  
Fluorescein Isothiocyanate ◽  
Phase Protein ◽  
Protein Sequencer

scholarly journals Amino acid sequence of versutoxin, a lethal neurotoxin from the venom of the funnel-web spider Atrax versutus

1988 ◽  
Vol 250 (2) ◽  
pp. 401-405 ◽  
Author(s):  
M R Brown ◽  
D D Sheumack ◽  
M I Tyler ◽  
M E H Howden
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Gas Phase ◽  
Edman Degradation ◽  
Amino Acid Residues ◽  
Single Chain ◽  
Male And Female ◽  
Web Spider ◽  
Phase Protein ◽  
Protein Sequencer

The complete amino acid sequence of versutoxin, a lethal neurotoxic polypeptide isolated from the venom of male and female funnel-web spiders of the species Atrax versutus, was determined. Sequencing was performed in a gas-phase protein sequencer by automated Edman degradation of the S-carboxymethylated toxin and fragments of it produced by reaction with CNBr. Versutoxin consisted of a single chain of 42 amino acid residues. It was found to have a high proportion of basic residues and of cystine. The primary structure showed marked homology with that of robustoxin, a novel neurotoxin recently isolated from the venom of another funnel-web-spider species, Atrax robustus.

The amino acid sequence of human sperm protamine P1

1985 ◽  
Vol 5 (5) ◽  
pp. 383-391 ◽  
Author(s):  
D. J. McKay ◽  
B. S. Renaux ◽  
G. H. Dixon
Keyword(s):  
Molecular Weight ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Gas Phase ◽  
Human Sperm ◽  
Calculated Molecular Weight ◽  
Phase Protein ◽  
Protein Sequencer

Human sperm protamines have been extracted from spermatozoa pooled from several donors, converted to their S-pyridylethylated derivatives and resolved into two major components, P1 and PI, by Bio-Rex 70 chromatography. Protamine P1 was further purified by Bio-Gel P-10 chromatography and sequenced directly on a gas phase protein sequencer for 43 residues. To complete the sequence, P1 was cleaved at methionine 36 and the C-terminal tetradecapeptide was purified by h.p.i.c, and sequenced completely. The 50 residue sequence is: 10 20 30 40 ARYRC CRSQS RSRYY RQRQR SRRRR RRSCQ TRRRA MRCCR 50 PRYRP RCRRH. This sequence has a calculated molecular weight of 6674 and is homologous with four other published mammalian protamine sequences.

Visible and ultraviolet spectroscopy of gas phase protein ions

2011 ◽  
Vol 13 (37) ◽  
pp. 16494 ◽  
Author(s):  
Rodolphe Antoine ◽  
Philippe Dugourd
Keyword(s):  
Gas Phase ◽  
Ultraviolet Spectroscopy ◽  
Protein Ions ◽  
Phase Protein
Sign in / Sign up

Export Citation Format

Share Document