scholarly journals Gas-phase protein salt bridge stabilities from collisional activation and electron transfer dissociation

2017 ◽  
Vol 420 ◽  
pp. 51-56 ◽  
Author(s):  
Zhe Zhang ◽  
Richard W. Vachet
Keyword(s):  
Electron Transfer ◽  
Gas Phase ◽  
Collisional Activation ◽  
Electron Transfer Dissociation ◽  
Salt Bridge ◽  
Phase Protein

Ion-Ion interactions affect (re)folding dynamics of ubiquitin ions in the gas phase. Distinct folding intermediates formed via charge-reduction pathways

2017 ◽  
Author(s):  
Jacquelyn R. Jhingree ◽  
Perdita E. Barran
Keyword(s):  
Electron Transfer ◽  
Gas Phase ◽  
Cross Sections ◽  
Structural Integrity ◽  
Salt Bridge ◽  
Charge Reduction ◽  
Net Charge ◽  
Ion Interactions ◽  
Folding Dynamics ◽  
Low Charge

<p>Using Ubiquitin as an exemplar protein we examine the effect of net charge reduction post electrospray ionisation by exposure to the electron transfer reagent, 1,3-dicyanobenzene. We monitor the change in gas phase conformation of both precursor and products with ion mobility mass spectrometry (IM-MS). Dramatic conformational rearrangement is seen for low charge state ions upon exposure to the electron transfer reagent. Ions with low charge states sprayed from both native-like and denaturing solvent conditions undergo structural transitions to conformers with cross sections in the range measured for the native structure (<sup>TW</sup>CCS<sub>N2</sub><sub>®</sub><sub>He</sub>, 950-1000 Å<sup>2</sup>). Thus, we infer that some memory of the solution phase structure is retained in the gas phase. Intermediate structures are seen in the reduction of the [M+6H]<sup>6+</sup> ion sprayed from both native-like and denaturing solvents. Further, the reduction pathway of this ion shows compaction to structures with a <sup>TW</sup>CCS<sub>N2</sub><sub>®</sub><sub>He</sub> centred at 1069 Å<sup>2</sup> (5+) and 949 Å<sup>2</sup> (4+) for ions originating from native-like and denaturing solvents respectively. We propose that charge reduction sites for radical anion localisation (to effect electron transfer) are not easily accessible in the case of ubiquitin molecules originating from native-like solution conditions. This highlights the importance of salt bridge interactions in maintaining the structural integrity of a protein in the gas phase. Most interestingly, two distinct conformer populations are seen for the 6+ charge-reduced product originating from the 7+ and, the 6+ exposed to radical anions (post ESI); we infer that these populations are intermediate in the refolding of ubiquitin in the gas phase, sometimes transient. Overall, we are able to monitor the refolding pathway of ubiquitin in the gas phase as its charge is reduced and show that charge-charge interactions play a significant role in the gas phase conformation adopted; whereby specific conformations are formed. </p>

Ion-Ion interactions affect (re)folding dynamics of ubiquitin ions in the gas phase. Distinct folding intermediates formed via charge-reduction pathways

2017 ◽  
Author(s):  
Jacquelyn R. Jhingree ◽  
Perdita E. Barran
Keyword(s):  
Electron Transfer ◽  
Gas Phase ◽  
Cross Sections ◽  
Structural Integrity ◽  
Salt Bridge ◽  
Charge Reduction ◽  
Net Charge ◽  
Ion Interactions ◽  
Folding Dynamics ◽  
Low Charge

<p>Using Ubiquitin as an exemplar protein we examine the effect of net charge reduction post electrospray ionisation by exposure to the electron transfer reagent, 1,3-dicyanobenzene. We monitor the change in gas phase conformation of both precursor and products with ion mobility mass spectrometry (IM-MS). Dramatic conformational rearrangement is seen for low charge state ions upon exposure to the electron transfer reagent. Ions with low charge states sprayed from both native-like and denaturing solvent conditions undergo structural transitions to conformers with cross sections in the range measured for the native structure (<sup>TW</sup>CCS<sub>N2</sub><sub>®</sub><sub>He</sub>, 950-1000 Å<sup>2</sup>). Thus, we infer that some memory of the solution phase structure is retained in the gas phase. Intermediate structures are seen in the reduction of the [M+6H]<sup>6+</sup> ion sprayed from both native-like and denaturing solvents. Further, the reduction pathway of this ion shows compaction to structures with a <sup>TW</sup>CCS<sub>N2</sub><sub>®</sub><sub>He</sub> centred at 1069 Å<sup>2</sup> (5+) and 949 Å<sup>2</sup> (4+) for ions originating from native-like and denaturing solvents respectively. We propose that charge reduction sites for radical anion localisation (to effect electron transfer) are not easily accessible in the case of ubiquitin molecules originating from native-like solution conditions. This highlights the importance of salt bridge interactions in maintaining the structural integrity of a protein in the gas phase. Most interestingly, two distinct conformer populations are seen for the 6+ charge-reduced product originating from the 7+ and, the 6+ exposed to radical anions (post ESI); we infer that these populations are intermediate in the refolding of ubiquitin in the gas phase, sometimes transient. Overall, we are able to monitor the refolding pathway of ubiquitin in the gas phase as its charge is reduced and show that charge-charge interactions play a significant role in the gas phase conformation adopted; whereby specific conformations are formed. </p>

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