Virulence properties of escherichia coli strains in patients with chronic pyelonephritis

Infection ◽  
1986 ◽  
Vol 14 (3) ◽  
pp. 145-150 ◽  
Author(s):  
R. Fünfstück ◽  
G. Stein ◽  
G. Wessel ◽  
H. Tschäpe ◽  
H. Kunath ◽  
...  
1994 ◽  
Vol 8 (1) ◽  
Author(s):  
G. Schumacher ◽  
H. Steinrûck ◽  
A. Geyid ◽  
B. Kollberg ◽  
Å. Ljungh

2018 ◽  
Vol 40 (6) ◽  
pp. 625-634 ◽  
Author(s):  
Dong Ho Kim ◽  
Bindu Subhadra ◽  
Hee Young Kang ◽  
Kyungho Woo ◽  
Jaeseok Kim ◽  
...  

2015 ◽  
Vol 72 (1) ◽  
pp. 19-28 ◽  
Author(s):  
João Paulo Lopes de Andrade ◽  
Luiz de Macêdo Farias ◽  
João Fernando Gonçalves Ferreira ◽  
Oscar Bruna-Romero ◽  
Daniele da Glória de Souza ◽  
...  

2001 ◽  
Vol 39 (5) ◽  
pp. 2017-2021 ◽  
Author(s):  
S. P. Djordjevic ◽  
M. A. Hornitzky ◽  
G. Bailey ◽  
P. Gill ◽  
B. Vanselow ◽  
...  

1998 ◽  
Vol 36 (6) ◽  
pp. 1604-1607 ◽  
Author(s):  
L. H. Wieler ◽  
Anja Schwanitz ◽  
Elke Vieler ◽  
Barbara Busse ◽  
H. Steinrück ◽  
...  

Shiga toxin-producing Escherichia coli (STEC) strains of serogroup O118 are the most prevalent group among STEC strains in diarrheic calves in Germany (L. H. Wieler, Ph.D. thesis, University of Giessen, 1997). To define their virulence properties, 42 O118 (O118:H16 [n = 38] and O118:H− [n = 4]) strains were characterized. The strains displayed three different Stx combinations (Stx1 [36 of 42], Stx1 and Stx2 [2 of 42], and Stx2 [4 of 42]). A total of 41 strains (97.6%) harbored a large virulence-associated plasmid containinghly EHEC (hly from enterohemorrhagicE. coli). The strains’ adhesive properties varied in relation to the eukaryotic cells tested. Only 28 of 42 strains (66.7%) showed localized adhesion (LA) in the human HEp-2 cell line. In contrast, in bovine fetal calf lung (FCL) cells, the number of LA-positive strains was much higher (37 of 42 [88.1%]). The locus of enterocyte effacement (LEE) was detected in 41 strains (97.6%). However, not all LEE-positive strains reacted positively in the fluorescence actin-staining (FAS) test, which indicated the attaching and effacing (AE) lesion. In HEp-2 cells, only 22 strains (52.4%) were FAS positive, while in FCL cells, the number of FAS-positive strains was significantly higher (38 of 42 [90.5%; P < 0.001]). In conclusion, the vast majority of the O118 STEC strains from calves (41 of 42 [97.6%]) have a high virulence potential (stx, hly EHEC, and LEE). This virulence potential and the high prevalence of STEC O118 strains in calves suggest that these strains could be a major health threat for humans in the future. In addition, the poor association between results of the geno- and phenotypical tests to screen for the AE ability of STEC strains calls the diagnostic value of the FAS test into question.


2004 ◽  
Vol 67 (10) ◽  
pp. 2117-2122 ◽  
Author(s):  
HIRONO TOSHIMA ◽  
EIKO UENAKA ◽  
YING BI ◽  
HIROMI NAKAMURA ◽  
JUN OGASAWARA ◽  
...  

Enteroaggregative Escherichia coli heat-stable enterotoxin 1 (EAST1) was originally regarded as a putative enterotoxin of enteroaggregative Escherichia coli. Although its etiological role has not yet been elucidated, it has been epidemiologically suggested that some strains of E. coli possessing EAST1-coding gene (astA) but no other identifiable pathogenic properties comprise a new group of diarrhea-associated E. coli (EAST1EC). However, the source of the organisms and their prevalence in foods are still obscure. In this study, methods for detection of the organisms in foods heavily contaminated with coliforms were evaluated and properties of the isolated strains were compared with those of fecal strains. Four enrichment methods (brilliant green lactose bile broth, E. coli, lauryl tryptose broth, and a combination of brain heart infusion broth and tryptone phosphate broth) were evaluated through inspection of 115 samples. PCR showed positive results in 26 samples after enrichment with a combination of brain heart infusion broth and tryptone phosphate broth, and EAST1EC was successfully isolated from 18 samples. Fifteen samples showed a positive reaction in the PCR test after enrichment by the other methods, and the organisms were isolated from only 10 specimens. The highest prevalence of EAST1EC was found in animal products (16 of 54, 29.6%); the organism was rarely found in foods of plant origin (2 of 45, 4.4%) or fishery products (1 of 16, 6.3%). Although EAST1EC is unexpectedly common in animal products, its potential as a human pathogen remains uncertain because the possession of some virulence properties differs significantly between strains from fecal specimens and those from foods. Some food isolates, however, possess the same characteristics as diarrheal isolates do. It is necessary to clarify the pathogenicity of EAST1EC and the significance of food as a source of infection.


2003 ◽  
Vol 69 (4) ◽  
pp. 406-410 ◽  
Author(s):  
ELISABETH PRESTERL ◽  
PETER G. KREMSNER ◽  
SONJA REICHMANN ◽  
RALPH H. ZWICK ◽  
STEFAN WINKLER ◽  
...  

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