Column selection of antigenic variants from tumors. YAC (Moloney) lymphoma variants with reduced antigen expression

1979 ◽  
Vol 9 (1) ◽  
pp. 465-475 ◽  
Author(s):  
Tina Dalianis ◽  
George Klein ◽  
Birger Andersson
Keyword(s):  
Antigen Expression ◽  
Selection Of

scholarly journals Depletion of CD8+ cells in human thymic medulla results in selective immune deficiency.

1989 ◽  
Vol 170 (6) ◽  
pp. 2177-2182 ◽  
Author(s):  
C M Roifman ◽  
D Hummel ◽  
H Martinez-Valdez ◽  
P Thorner ◽  
P J Doherty ◽  
...  
Keyword(s):  
T Cells ◽  
Peripheral Blood ◽  
Inbred Mice ◽  
Pneumocystis Carinii ◽  
Antigen Expression ◽  
Class I ◽  
Class I Mhc ◽  
Cd8 Cells ◽  
Mhc Antigen ◽  
Selection Of

CD8 molecules expressed on the surface of a subset of T cells participate in the selection of class I MHC antigen-restricted T cells in the thymus, and in MHC-restricted immune responses of mature class I MHC antigen-restricted T cells. Here we describe an immune-deficient patient with lack of CD8+ peripheral blood cells. The patient presented with Pneumocystis carinii pneumonia and was unable to reject an allogeneic skin graft, but had normal primary and secondary antibody responses. Examination of the patient's thymus revealed that the loss of CD8+ cells occurred during intrathymic differentiation: the patient's immature cortical thymocytes included both CD4+ and CD8+ cells while the mature medullary cells expressed the CD4 but not the CD8 protein on their surface. Northern blot and polymerase chain reaction analyses revealed the presence of CD8 alpha and beta mRNA in the patient's thymus but not in the peripheral blood. Both class I MHC antigen expression and the expressed TCR V beta repertoire are normal in this patient. These data are consistent with an impaired selection of CD8+ cells in the patient's thymus and support the role of the CD8 surface protein in thymic selection previously characterized in genetically manipulated and inbred mice.

scholarly journals Global selection of Plasmodium falciparum virulence antigen expression by host antibodies

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Abdirahman I. Abdi ◽  
George M. Warimwe ◽  
Michelle K. Muthui ◽  
Cheryl A. Kivisi ◽  
Esther W. Kiragu ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Antigen Expression ◽  
Global Selection ◽  
Virulence Antigen ◽  
Selection Of

scholarly journals Serological Detection of Rh-Del Phenotype among Rh-Negative Blood Donors at National Blood Center, Yangon, Myanmar

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Saw Thu Wah ◽  
Saung Nay Chi ◽  
Kyi Kyi Kyaing ◽  
Aye Aye Khin ◽  
Thida Aung
Keyword(s):  
Molecular Markers ◽  
Genetic Polymorphism ◽  
Blood Donors ◽  
Antigen Expression ◽  
Red Cell ◽  
Serological Detection ◽  
Blood Center ◽  
Elution Method ◽  
Selection Of ◽  
Positive Pool

Background. Red cell Rhesus (Rh) antigen expression is influenced by the genetic polymorphism of RHD and RHCE genes and reveals serologically different reactions of RhD variants such as partial D, weak D, and Rh-Del. Serologically, Rh-Del type can only be detected by an adsorption-elution technique, and it might be mistyped as Rh-negative. The prevalence of Rh-Del has not been reported yet in Myanmar. Method. A total of 222 Rh-negative blood donors in the National Blood Center were tested for weak D and Rh-Del by indirect antihuman globulin and adsorption-elution method, respectively. RhCE typing was performed among Rh-negative and Rh-Del. Results. Of them, 75.2% (167/222) were Rh-negative, 15.8% (35/222) were Rh-Del, and 9% (20/222) were weak D. Of 202 blood donors (167 true Rh-negative and 35 Rh-Del), all of the Rh-Del positives were C-antigen-positive with 94.3% Ccee phenotype (33/35) and 5.7% CCee (2/35). Most of the Rh-negative donors (80.2%) were ccee phenotype (134/167). Conclusion. About half of Rh-Del subjects were repeated donors, and attention was needed to avoid transfusion of truly Rh-negative patients to prevent alloimmunization. It is recommended to do Rh-Del typing of Rh-negative donors who are C-antigen-positive and consider moving them to the Rh-positive pool. Further study is needed to clarify the alloimmunization status for transfusion of Rh-Del blood to Rh-negative recipients. Molecular markers for RhD-negative and D variants should be established in the Myanmar population to improve selection of antisera for Rh typing and enhance safety of the transfusion services.

scholarly journals Selection of benign primitive hematopoietic progenitors in chronic myelogenous leukemia on the basis of HLA-DR antigen expression

Blood ◽  
1992 ◽  
Vol 79 (4) ◽  
pp. 1003-1010 ◽  
Author(s):  
CM Verfaillie ◽  
WJ Miller ◽  
K Boylan ◽  
PB McGlave
Keyword(s):  
Bone Marrow ◽  
Chronic Myelogenous Leukemia ◽  
Autologous Bone Marrow Transplantation ◽  
Antigen Expression ◽  
Myelogenous Leukemia ◽  
Hematopoietic Progenitors ◽  
Hematopoietic Stem ◽  
Hla Dr ◽  
Selection Of ◽  
Clonogenic Cells

Abstract Chronic myelogenous leukemia (CML) is a lethal malignancy of the human hematopoietic stem cell. Here we report that coexistent benign, primitive hematopoietic progenitors can be distinguished from their malignant counterparts in CML bone marrow by differences in cell surface antigen expression. Selection of bone marrow cells expressing the CD34 antigen but lacking the HLA-DR antigen results in recovery of small lymphocyte-like blasts, which initiate and sustain production of myeloid clonogenic progeny in vitro. Secondary clonogenic cells derived at week 1, 5, and 8 from long-term bone marrow cultures (LTBMCs) initiated with primitive progenitors, which lack HLA-DR antigens, exhibit neither the Philadelphia chromosome (Ph1) nor the corresponding bcr/abl mRNA characteristic of CML. In contrast, clonogenic cells recovered at week 1, 5, and 8 from LTBMCs initiated with the CML HLA- DR+ population contain Ph1 and express bcr/abl mRNA. This observation indicates that it may be possible to select a population of viable, exclusively benign hematopoietic stem cells from CML bone marrow capable of repopulating the hematopoietic compartment following autologous bone marrow transplantation.

Immunological selection of tumour cells which have lost SV40 antigen expression

Nature ◽  
1977 ◽  
Vol 269 (5623) ◽  
pp. 36-40 ◽  
Author(s):  
P. T. Mora ◽  
C. Chang ◽  
L. Couvillion ◽  
J. M. Kuster ◽  
V. W. McFarland
Keyword(s):  
Antigen Expression ◽  
Tumour Cells ◽  
Selection Of

ER, PR, and HER2 assessment in Hispanic women with breast cancer: A comparison between IHC and AQUA technology.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e11568-e11568
Author(s):  
Anna Maria Di Marco ◽  
Jose Alberto Ruiz ◽  
Maryangely Moreno ◽  
Carlos El Hader ◽  
Luis Baez
Keyword(s):  
Breast Cancer ◽  
Ductal Carcinoma ◽  
Hispanic Women ◽  
Antigen Expression ◽  
Tumor Grade ◽  
Her2 Status ◽  
Number Of Patients ◽  
Discordance Rate ◽  
Appropriate Therapy ◽  
Selection Of

e11568 Background: Estrogen (ER), progesterone (PR) receptors and HER2 are important markers for breast cancer (BC) treatment planning. These markers are often measured by immunochemistry (IHC) but IHC is subject to limitations of operator subjectivity and equivocal results making selection of appropriate therapy difficult and ultimately affecting patient outcome. Methods: 135 females with BC were recruited in Puerto Rico between Sept and Dec 2012. Median patient age was 57 and 95 % had invasive ductal carcinoma. Our aim was to compare discordance rates of ER, PR and HER2 between IHC and AQUA, an automated fluorescent technology that quantifies antigen expression, in a population of Hispanic women and to investigate whether specimen type or tumor grade influence discordance rate between the two technologies. Results: 103 of 135 patient samples were found to be ER+ by both technologies and 28 samples were ER- by both IHC and AQUA. 4 samples (3%) were discordant; 2 each were AQUA+/IHC- and AQUA-/IHC+. 73 of 135 samples expressed PR by both technologies and 55 samples were PR- by both IHC and AQUA. 14 samples (10%) were discordant; 7 each were AQUA+/IHC- and AQUA-/IHC+. 87 of 135 samples were HER2- and 13 patients were found to be HER2+ by both technologies. However 35 samples (26%) were discordant for HER2 status; 31 were AQUA+/IHC- while 4 AQUA-/IHC+. 100 of the HER2 results were scored 0-1 (negative) by IHC and 25 % of these cases were overturned by AQUA to HER2 +. 19 of the HER2 results were scored 2 (borderline) by IHC. 32 % of these cases were positive by AQUA. Conclusions: Discordance rate were 3% for ER, 10 % for PR but 26% for HER2 between IHC and AQUA. The Cohen’s Kappa coefficient for ER and PR were 0.914 and 0.785 respectively but only 0.299 for HER2 (p values < 0.00001 for all 3 values). Needle/core biopsy yielded more HER2 discordance than lumpectomy/mastectomy (31% vs. 16%). HER2 discordance between IHC and AQUA was more common in higher grade tumors. The large number of HER2 negative IHC cases scored positive by AQUA, and considering the data demonstrating that HER2 by AQUA better predicts benefit from trastuzumab (Slamon D, SABCS 2012) suggests that a significant additional number of patients could benefit from such targeted therapy.

scholarly journals Major histocompatibility complex antigen expression on the epithelium of the developing thymus in normal and nude mice.

1981 ◽  
Vol 153 (2) ◽  
pp. 280-292 ◽  
Author(s):  
E J Jenkinson ◽  
W Van Ewijk ◽  
J J Owen
Keyword(s):  
Monoclonal Antibodies ◽  
Major Histocompatibility Complex ◽  
Nude Mice ◽  
Antigen Expression ◽  
Major Histocompatibility Complex Antigen ◽  
Major Histocompatibility ◽  
Histocompatibility Complex ◽  
Selection Of

The expression and distribution of antigens coded by the K and I regions of the major histocompatibility complex in the developing thymus of normal and nude mice has been investigated using monoclonal antibodies. Both immunohistological studies of intact rudiments and in vitro labeling of cultures derived from microdissected rudiments indicate that, while K region antigens are present on epithelial and mesenchymal elements, I region antigens are only detectable on the epithelium. This view is also substantiated by the selective absence of I region antigens in the abnormal nude thymic rudiment where the defect is considered to be epithelial in nature. The findings are considered in relation to the role of the thymus in providing an environment for the differentiation and selection of developing T cells, and it is proposed that the Ia-expressing epithelial elements play a central role in these functions.

scholarly journals Selection of phenotypically distinct NK1.1+ T cells upon antigen expression in the thymus or in the liver

1999 ◽  
Vol 29 (7) ◽  
pp. 2330-2343 ◽  
Author(s):  
Valérie Legendre ◽  
Claude Boyer ◽  
Sylvie Guerder ◽  
Bernd Arnold ◽  
Günter Hämmerling ◽  
...  
Keyword(s):  
T Cells ◽  
Antigen Expression ◽  
Selection Of

scholarly journals Selection of benign primitive hematopoietic progenitors in chronic myelogenous leukemia on the basis of HLA-DR antigen expression

Blood ◽  
1992 ◽  
Vol 79 (4) ◽  
pp. 1003-1010 ◽  
Author(s):  
CM Verfaillie ◽  
WJ Miller ◽  
K Boylan ◽  
PB McGlave
Keyword(s):  
Bone Marrow ◽  
Chronic Myelogenous Leukemia ◽  
Autologous Bone Marrow Transplantation ◽  
Antigen Expression ◽  
Myelogenous Leukemia ◽  
Hematopoietic Progenitors ◽  
Hematopoietic Stem ◽  
Hla Dr ◽  
Selection Of ◽  
Clonogenic Cells

Chronic myelogenous leukemia (CML) is a lethal malignancy of the human hematopoietic stem cell. Here we report that coexistent benign, primitive hematopoietic progenitors can be distinguished from their malignant counterparts in CML bone marrow by differences in cell surface antigen expression. Selection of bone marrow cells expressing the CD34 antigen but lacking the HLA-DR antigen results in recovery of small lymphocyte-like blasts, which initiate and sustain production of myeloid clonogenic progeny in vitro. Secondary clonogenic cells derived at week 1, 5, and 8 from long-term bone marrow cultures (LTBMCs) initiated with primitive progenitors, which lack HLA-DR antigens, exhibit neither the Philadelphia chromosome (Ph1) nor the corresponding bcr/abl mRNA characteristic of CML. In contrast, clonogenic cells recovered at week 1, 5, and 8 from LTBMCs initiated with the CML HLA- DR+ population contain Ph1 and express bcr/abl mRNA. This observation indicates that it may be possible to select a population of viable, exclusively benign hematopoietic stem cells from CML bone marrow capable of repopulating the hematopoietic compartment following autologous bone marrow transplantation.

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