scholarly journals Serological Detection of Rh-Del Phenotype among Rh-Negative Blood Donors at National Blood Center, Yangon, Myanmar

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Saw Thu Wah ◽  
Saung Nay Chi ◽  
Kyi Kyi Kyaing ◽  
Aye Aye Khin ◽  
Thida Aung
Keyword(s):  
Molecular Markers ◽  
Genetic Polymorphism ◽  
Blood Donors ◽  
Antigen Expression ◽  
Red Cell ◽  
Serological Detection ◽  
Blood Center ◽  
Elution Method ◽  
Selection Of ◽  
Positive Pool

Background. Red cell Rhesus (Rh) antigen expression is influenced by the genetic polymorphism of RHD and RHCE genes and reveals serologically different reactions of RhD variants such as partial D, weak D, and Rh-Del. Serologically, Rh-Del type can only be detected by an adsorption-elution technique, and it might be mistyped as Rh-negative. The prevalence of Rh-Del has not been reported yet in Myanmar. Method. A total of 222 Rh-negative blood donors in the National Blood Center were tested for weak D and Rh-Del by indirect antihuman globulin and adsorption-elution method, respectively. RhCE typing was performed among Rh-negative and Rh-Del. Results. Of them, 75.2% (167/222) were Rh-negative, 15.8% (35/222) were Rh-Del, and 9% (20/222) were weak D. Of 202 blood donors (167 true Rh-negative and 35 Rh-Del), all of the Rh-Del positives were C-antigen-positive with 94.3% Ccee phenotype (33/35) and 5.7% CCee (2/35). Most of the Rh-negative donors (80.2%) were ccee phenotype (134/167). Conclusion. About half of Rh-Del subjects were repeated donors, and attention was needed to avoid transfusion of truly Rh-negative patients to prevent alloimmunization. It is recommended to do Rh-Del typing of Rh-negative donors who are C-antigen-positive and consider moving them to the Rh-positive pool. Further study is needed to clarify the alloimmunization status for transfusion of Rh-Del blood to Rh-negative recipients. Molecular markers for RhD-negative and D variants should be established in the Myanmar population to improve selection of antisera for Rh typing and enhance safety of the transfusion services.

scholarly journals SEROPREVALENCE OF CHAGASIC INFECTION IN YOUNG INDIVIDUALS IN A BLOOD CENTER IN THE STATE OF SAO PAULO, BRAZIL

2013 ◽  
Vol 55 (4) ◽  
pp. 245-250 ◽  
Author(s):  
Elaine Cristina Navarro ◽  
Renata Leme Goto ◽  
Isabella Silva Ricoboni ◽  
Jose Eduardo Corrente ◽  
Rita Maria Saccomano Henriques ◽  
...  
Keyword(s):  
Public Health ◽  
Chagas Disease ◽  
Blood Donors ◽  
Public Health Problem ◽  
Sao Paulo ◽  
São Paulo ◽  
Blood Center ◽  
Negative Results ◽  
Central Regions ◽  
The City

SUMMARY This study aimed at estimating the number of cases of non-negative serological reactions to Chagas disease in blood donors at the Blood Center of Botucatu, São Paulo, Brazil, from 2003 to 2010 and at relating them to their cities of origin. Five hundred and seventy-four non-negative results for Chagas disease were evaluated. Of these, 371 (64.8%) were reagent, and 203 (35.4%) were inconclusive. The prevalence of Chagas disease in blood donors was 0.05%. There were, on average, 72 cases/year, and a prevalence of males was observed (64.8%). Forty-three (7.49%) individuals were 18 to 30 years old; 92 (16.02%) were 31 to 40; 147 (25.61%) 41 to 50, and 292 (50.87%) were older than 50 years. It was observed that 29.3% of females with reagent serology were at their fertile age (18 and 45 years). The majority of donors were originally from cities in the southwestern and central regions of São Paulo, but individuals from other states contributed with 20%. The provenance of most donors was the city of Botucatu/SP, followed by the city of Taquarituba/SP. Therefore, the profile of donors at this blood center favors the occurrence of a larger number of non-negative serological reactions. Although there has been a significant reduction in the number of new cases/year for this disease, it is still a public-health problem, and results suggest the need for new epidemiological assessments in the studied region.

scholarly journals Genetic Polymorphism of SCD1 Gene of Holstein-Friesian Cows in Indonesia

2019 ◽  
Vol 24 (2) ◽  
pp. 56
Author(s):  
Ari Sulistyo Wulandari ◽  
HD Rahayu ◽  
SD Volkandari ◽  
N Herlina ◽  
S Anwar ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Genetic Polymorphism ◽  
Breeding Strategy ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
Hardy Weinberg Equilibrium ◽  
Molecular Selection ◽  
Friesian Cattle ◽  
Selection Of ◽  
Friesian Cows

<p class="abstrak2">Stearoyl-Coenzyme A desaturase 1 (SCD1) belongs to the fatty acid family of desaturases. In lactating ruminants, the SCD1 protein is highly expressed in the mammary gland and is relevant for the fatty acid composition of milk and dairy products. Polymorphism of SCD1 gene in Holstein-Friesian (HF) cows could be used as a basis of molecular selection of cattle in order to increase their productivity. The aim of this study was to investigate the polymorphism of SCD1 gene of Holstein-Friesian cows in Indonesia. A total of 162 blood samples of HF cows were collected from four different locations i.e. Bogor, Sukabumi, Tasikmalaya and Enrekang districts. Genotyping of SCD1 gene used PCR-RFLP method with NcoI restriction enzyme. The result showed that three genotypes (AA, AV and VV) and two alleles (A and V) have successfully found and polymorphic. A allele was dominant in all populations (0.63) and in Hardy Weinberg Equilibrium. The highest A allele was found in Sukabumi (0.78) and the lowest was in Bogor (0.55). Heterozigosity observed and expected reached 0.471 and 0.470, respectively. In conclusion, genetic polymorphism was found in all population with dominant of A allele. This finding can be used as a early genetic information of Holstein-Friesian cattle in Indonesia and to build breeding strategy for improving of productivity especially improving of healthy fat milk. </p><p class="abstrak2"><span><br /></span></p>

Evaluation of a Malaria Antibody ELISA and Its Value in Reducing Potential Wastage of Red Cell Donations from Blood Donors Exposed to Malaria, with a Note on a Case of Transfusion- Transmitted Malaria

Vox Sanguinis ◽  
1997 ◽  
Vol 73 (3) ◽  
pp. 143-148 ◽  
Author(s):  
P. L. (a) Chiodini ◽  
S. (b) Hartley ◽  
P. E. (b) Hewitt ◽  
J. A. J. (b) Barbara ◽  
K. (a) Lalloo ◽  
...  
Keyword(s):  
Blood Donors ◽  
Red Cell ◽  
Antibody Elisa ◽  
Reducing Potential

scholarly journals Therapeutic Phlebotomy for Testosterone-Induced Polycythemia

2020 ◽  
Vol 154 (1) ◽  
pp. 33-37
Author(s):  
Nancy L Van Buren ◽  
Anita J Hove ◽  
Tracy A French ◽  
Jed B Gorlin
Keyword(s):  
Replacement Therapy ◽  
Blood Donors ◽  
Testosterone Replacement ◽  
Testosterone Replacement Therapy ◽  
Blood Center ◽  
The Impact

Abstract Objectives To evaluate therapeutic phlebotomy (TP) requests for testosterone replacement therapy (TRT) and to highlight the impact to a blood center (BC) or service that provides TP for individuals on TRT. Methods Review of TP requests for individuals on TRT at our BC over a 3-year period from 2014 through 2016, as well as the total number of TP collections. Results Total TPs during 2014, 2015, and 2016 were 475, 500, and 569, respectively. Annual TP collections for patients on TRT were 193, 212, and 239, respectively. TRT patients with TP orders increased 71.4% during this period. After discontinuation of TP services for TRT at our BC, 32% continued to donate as volunteer blood donors at our BC. Conclusions Our BC observed increased TP requests for patients on TRT from 2014 through 2016. Our findings suggest that individuals on TRT may be presenting to BCs as volunteer blood donors to avoid charges for TP.

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