Effect of gene dosage on cell-surface expression of Thy-1 antigen in somatic cell hybrids between Thy-1− abelson-leukemia-virus induced lymphomas and Thy-1+ mouse lymphomas

1981 ◽  
Vol 12 (1) ◽  
pp. 381-395 ◽  
Author(s):  
Robert Hyman ◽  
Kristie Cunningham ◽  
Valerie Stallings
Keyword(s):  
Cell Surface ◽  
Somatic Cell ◽  
Gene Dosage ◽  
Leukemia Virus ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

scholarly journals Reduced Cell Surface Expression of CCR5 in CCR5Δ32 Heterozygotes Is Mediated by Gene Dosage, Rather Than by Receptor Sequestration

2001 ◽  
Vol 277 (3) ◽  
pp. 2287-2301 ◽  
Author(s):  
Sundararajan Venkatesan ◽  
Ana Petrovic ◽  
Donald I. Van Ryk ◽  
Massimo Locati ◽  
Drew Weissman ◽  
...  
Keyword(s):  
Cell Surface ◽  
Gene Dosage ◽  
Surface Expression ◽  
Cell Surface Expression

scholarly journals Dileucine and YXXL Motifs in the Cytoplasmic Tail of the Bovine Leukemia Virus Transmembrane Envelope Protein Affect Protein Expression on the Cell Surface

2004 ◽  
Vol 78 (15) ◽  
pp. 8301-8311 ◽  
Author(s):  
Sinisa Novakovic ◽  
Earl T. Sawai ◽  
Kathryn Radke
Keyword(s):  
Cell Surface ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Surface Display ◽  
Chimeric Protein ◽  
Surface Expression ◽  
Terminal Segment ◽  
Cell Surface Expression ◽  
Chimeric Proteins ◽  
Env Protein

ABSTRACT Several retroviruses downmodulate the cell surface expression of envelope (Env) proteins through peptide sequences located in the cytoplasmic tail of the transmembrane (TM) subunit. We investigated whether cell surface expression of a chimeric protein containing the cytoplasmic domain of the TM protein (CTM) of bovine leukemia virus (BLV) was regulated by two membrane-proximal dileucine motifs or by tyrosine Y487 or Y498 in YXXL motifs. A chimeric protein composed of the extracellular and membrane-spanning portions of human CD8-α plus a wild-type (wt) BLV CTM was detectable on the surface of only 40% of the cells in which it was transiently expressed. Replacement of either dileucine pair with alanines increased the level of surface display of chimeric proteins. Nearly all cells became surface positive when both dileucine motifs were altered simultaneously and when either an N-terminal segment containing both dileucine motifs or a C-terminal segment containing all YXXL motifs was deleted. In contrast, replacement of Y487 or Y498 with alanine or phenylalanine enabled only small increases in surface display compared with wt levels. Chimeric proteins had similar stabilities but were downmodulated from the cell surface at three different rates. Point mutants segregated into each of the three groups of proteins categorized according to these different rates. Interestingly, Y487 mutants were downmodulated less efficiently than Y498 mutants, which behaved like wt. CD8-CTM chimeric proteins were phosphorylated on serine residues, but the native BLV Env protein was not phosphorylated either in transfected cells or in a lymphoid cell line constitutively producing BLV. Thus, both dileucine and YXXL motifs within the BLV CTM contribute to downmodulation of a protein containing this domain. Interactions with other proteins may influence surface exposure of Env protein complexes in virus-infected cells, assisting in viral evasion of adaptive immunity.

scholarly journals Increased synthesis and expression of H-2 antigens on thymocytes as a result of radiation leukemia virus infection: a possible mechanism for H-2 linked control of virus-induced neoplasia.

1978 ◽  
Vol 147 (2) ◽  
pp. 470-487 ◽  
Author(s):  
D Meruelo ◽  
S H Nimelstein ◽  
P P Jones ◽  
M Lieberman ◽  
H O McDevitt
Keyword(s):  
Virus Infection ◽  
Cell Surface ◽  
Leukemia Virus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Electrophoretic Analysis ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Altered Expression ◽  
Thymus Cells

Previous studies from this laboratory have mapped resistance and/or susceptibility to radiation-induced leukemia virus (RadLV)-induced neoplasia to the H-2D region. H-2 linked effects on virus replication can be detected subsequent to the initial virus infection, and clear-cut differences in numbers of virus infected thymus cells can be detected as early as 5 wk after RadLV inoculation. Rapid increases in cellular synthesis and cell surface expression of H-2 antigens are detectable immediately after virus inoculation. These changes have been studied by immunofluorescence, absorption, cell surface iodination followed by sodium dodecyl-sulfate-polyacrylamide gel electrophoresis, and two dimensional gel electrophoretic analysis of internally labeled lymphocyte proteins. Expression of H-2K molecules is significantly increased in cells of susceptible and resistant animals. However, significant increases in expression of H-2D antigens occurs only on thymus cells from resistant strains (H-2Dd). Transformed cells of resistant and susceptible H-2 haplotypes adapted to tissue culture lack detectable H-2 antigens as determined by serological absorption studies. It is argued that altered expression of H-2 antigens plays a very significant role in the mechanism of host defense to virus infection.

scholarly journals Cell Surface Expression of the Bovine Leukemia Virus-Binding Receptor on B and T Lymphocytes Is Induced by Receptor Engagement

2008 ◽  
Vol 181 (2) ◽  
pp. 891-898 ◽  
Author(s):  
Madakasira Lavanya ◽  
Sandrina Kinet ◽  
Amélie Montel-Hagen ◽  
Cédric Mongellaz ◽  
Jean-Luc Battini ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Cell Surface ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Virus Binding ◽  
B And T Lymphocytes

Variations in sarcoma and leukemia virus activity in somatic cell hybrids

1972 ◽  
Vol 10 (2) ◽  
pp. 310-319 ◽  
Author(s):  
Cedric Long ◽  
Gary Kelloff ◽  
Raymond V. Gilden
Keyword(s):  
Somatic Cell ◽  
Leukemia Virus ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Virus Activity
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