Chinese hamster � mouse hybrid cells segregating mouse chromosomes and isozymes

1975 ◽  
Vol 1 (4) ◽  
pp. 355-369 ◽  
Author(s):  
John D. Minna ◽  
Thomas H. Marshall ◽  
Patricia V. Shaffer-Berman
Keyword(s):  
Chinese Hamster ◽  
Hybrid Cells

Correlation between resistance to actinomycin D, karyology, agglutination by concanavalin A and tumorigenicity in Chinese hamster hybrid cells

1975 ◽  
Vol 18 (1) ◽  
pp. 67-77
Author(s):  
I. Imbert ◽  
Y. Barra ◽  
M. Berebbi
Keyword(s):  
Statistical Analysis ◽  
Concanavalin A ◽  
Actinomycin D ◽  
Marker Chromosome ◽  
Chinese Hamster ◽  
Inverse Correlation ◽  
Sensitive Strain ◽  
Hybrid Cells ◽  
Hybrid Line

Subclones isolated from a Chinese hamster hybrid line, derived from fusion of an actinomycin D-resistant and an actinomycin D-sensitive strain, were studied with respect to their resistance to actinomycin D, karyology, transplantability and agglutination by concanavalin A. Statistical analysis of the results allowed the establishment of a classification of the strains based on increasing resistance to actinomycin D. There appeared to be an inverse correlation between actinomycin D-resistance and tumorigenicity and a positive correlation between this resistance and the presence of a marker chromosome.

Analysis of the cell adhesion mechanism using somatic cell hybrids. I. Aggregation of hybrid cells between adhesive V79 and non-adhesive Ehrlich's ascites tumour cells

1980 ◽  
Vol 43 (1) ◽  
pp. 391-406
Author(s):  
H. Aoyama ◽  
T.S. Okada ◽  
M. Takeichi
Keyword(s):  
Cell Adhesion ◽  
Chinese Hamster ◽  
Hybrid Cells ◽  
Adhesive Properties ◽  
V79 Cells ◽  
Ascites Tumour ◽  
Independent Mechanism ◽  
Eat Cells ◽  
Hybrid Clones ◽  
Dependent Mechanism

V79 Chinese hamster cells dissociated with 1 nm EDTA retain 2 kinds of cell adhesion mechanisms, one dependent on Ca2+ and the other independent of Ca2+. Ehrlich's ascites tumour (EAT) cells are provided with neither Ca2+-dependent nor CA2+-independent mechanisms. Studies on the aggregation of cells of 25 different hybrid clones obtained by fusing these 2 lined cells revealed the following points with regard to adhesive properties of hybrid cells. (1) The activity of the Ca2+-independent mechanism was lower in most hybrid clones than in parental V79 cells. (2) There were a few hybrid clones whose Ca2+-dependent mechanism activity was lower than in V79 cells. In these clones, the Ca2+-independent mechanism was also less active than the parental cells. (3) A hybrid clone with reduced Ca2+-dependent mechanism activity only was not found. (4) All the hybrid clones have at least one set of chromosomes derived from V79. (5) The number of chromosomes derived from EAT cells tended to be less in hybrid clones with lower aggregative ability. These results can be explained by assuming that the activity of the Ca2+-independent and Ca2+-dependent mechanisms of V79 cells may be differently inhibited by genes contained in EAT cells.

Amphotericin B resistance is recessive in Chinese hamster hybrid cells

1981 ◽  
Vol 106 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Katsuhiko Hidaka ◽  
Shin-Ichi Akiyama ◽  
Michihiko Kuwano
Keyword(s):  
Amphotericin B ◽  
Chinese Hamster ◽  
Hybrid Cells

High-frequency reactivation of X-linked genes in chinese hamster � human hybrid cells

1987 ◽  
Vol 13 (3) ◽  
pp. 191-204 ◽  
Author(s):  
Nathan Ellis ◽  
Elisabeth Keitges ◽  
Stanley M. Gartler ◽  
Mariano Rocchi
Keyword(s):  
High Frequency ◽  
Chinese Hamster ◽  
Hybrid Cells

scholarly journals Focus-forming ability and surface markers of hamster-human malignant lymphoma hybrids

Blood ◽  
1980 ◽  
Vol 55 (2) ◽  
pp. 351-354 ◽  
Author(s):  
GB Price ◽  
JF Sturgeon ◽  
JE Till
Keyword(s):  
Malignant Lymphoma ◽  
Cho Cells ◽  
Blood Cells ◽  
Bone Marrow Cells ◽  
Chinese Hamster ◽  
Lymphocytic Leukemia ◽  
Surface Markers ◽  
Hybrid Cells ◽  
Immunologic Markers ◽  
Differentiation Status

Abstract We have examined the properties of hybrid cells formed by polyethylene glycol-mediated fusion of the GRC+L-73 line of Chinese hamster ovary (CHO) cells with peripheral blood cells from patients with chronic lymphocytic leukemia (CLL) or with bone marrow cells from patients with malignant lymphoma. The results indicate that hybrid cells can be detected by their ability to form “foci” of characteristic morphology in the presence of a monolayer of parental CHO cells and that clones isolated from such foci express aspects of the differentiation status, as detected by immunologic markers, of the human parental cells.

scholarly journals Canine and Feline Parvoviruses Can Use Human or Feline Transferrin Receptors To Bind, Enter, and Infect Cells

2001 ◽  
Vol 75 (8) ◽  
pp. 3896-3902 ◽  
Author(s):  
John S. L. Parker ◽  
William J. Murphy ◽  
Dai Wang ◽  
Stephen J. O'Brien ◽  
Colin R. Parrish
Keyword(s):  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Canine Parvovirus ◽  
Endocytic Pathway ◽  
Transferrin Receptors ◽  
Hybrid Cells ◽  
Cho Cell ◽  
Viral Capsids ◽  
Feline Panleukopenia Virus

ABSTRACT Canine parvovirus (CPV) enters and infects cells by a dynamin-dependent, clathrin-mediated endocytic pathway, and viral capsids colocalize with transferrin in perinuclear vesicles of cells shortly after entry (J. S. L. Parker and C. R. Parrish, J. Virol. 74:1919–1930, 2000). Here we report that CPV and feline panleukopenia virus (FPV), a closely related parvovirus, bind to the human and feline transferrin receptors (TfRs) and use these receptors to enter and infect cells. Capsids did not detectably bind or enter quail QT35 cells or a Chinese hamster ovary (CHO) cell-derived cell line that lacks any TfR (TRVb cells). However, capsids bound and were endocytosed into QT35 cells and CHO-derived TRVb-1 cells that expressed the human TfR. TRVb-1 cells or TRVb cells transiently expressing the feline TfR were susceptible to infection by CPV and FPV, but the parental TRVb cells were not. We screened a panel of feline-mouse hybrid cells for susceptibility to FPV infection and found that only those cells that possessed feline chromosome C2 were susceptible. The feline TfR gene (TRFC) also mapped to feline chromosome C2. These data indicate that cell susceptibility for these viruses is determined by the TfR.

scholarly journals SURFACE ANTIGENS OF MAMMALIAN CELLS AS GENETIC MARKERS. II

1973 ◽  
Vol 138 (1) ◽  
pp. 229-244 ◽  
Author(s):  
Paul Wuthier ◽  
Carol Jones ◽  
Theodore T. Puck
Keyword(s):  
Mammalian Cells ◽  
Human Lymphocytes ◽  
Human Fibroblasts ◽  
Chinese Hamster ◽  
Lactic Dehydrogenase ◽  
Hybrid Cells ◽  
Human Genes ◽  
Cultured Human Fibroblasts ◽  
Human Rbcs ◽  
Cell Plating

A second surface antigen, BL, lethal in the presence of specific antibody and complement has been identified on some human cells and shown to behave as a good genetic marker. It is autosomal, unlinked to the human AL antigen previously described, and unlinked to 15 other human genes. The AL antigen, which is linked to the lactic dehydrogenase A gene, is found on the HeLa, the cultured human fibroblast, and in small amounts on the human lymphocyte. BL occurs on HeLa cells, on cultured human fibroblasts, and on human lymphocytes, but not on human RBCs. Hybrid cells formed by fusion of human and Chinese hamster cells have been prepared containing each of the four possible combinations of these two markers. Highly selective antisera sensitive to each marker separately can be obtained. The use of single-cell plating to demonstrate the presence of the antigens and of hybrid cells containing desired combinations of the markers facilitates study in this system.

scholarly journals Focus-forming ability and surface markers of hamster-human malignant lymphoma hybrids

Blood ◽  
1980 ◽  
Vol 55 (2) ◽  
pp. 351-354
Author(s):  
GB Price ◽  
JF Sturgeon ◽  
JE Till
Keyword(s):  
Malignant Lymphoma ◽  
Cho Cells ◽  
Blood Cells ◽  
Bone Marrow Cells ◽  
Chinese Hamster ◽  
Lymphocytic Leukemia ◽  
Surface Markers ◽  
Hybrid Cells ◽  
Immunologic Markers ◽  
Differentiation Status

We have examined the properties of hybrid cells formed by polyethylene glycol-mediated fusion of the GRC+L-73 line of Chinese hamster ovary (CHO) cells with peripheral blood cells from patients with chronic lymphocytic leukemia (CLL) or with bone marrow cells from patients with malignant lymphoma. The results indicate that hybrid cells can be detected by their ability to form “foci” of characteristic morphology in the presence of a monolayer of parental CHO cells and that clones isolated from such foci express aspects of the differentiation status, as detected by immunologic markers, of the human parental cells.

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