Comparison of mitochondrially synthesized polypeptides of human, mouse, and monkey cell lines by a two-dimensional protease gel system

1984 ◽  
Vol 10 (6) ◽  
pp. 639-643
Author(s):  
Noelynn Oliver ◽  
Judith McCarthy ◽  
Douglas C. Wallace
Keyword(s):  
Cell Lines ◽  
Two Dimensional ◽  
Monkey Cell ◽  
Gel System

scholarly journals Protein expression profile related to cisplatin resistance in bladder cancer cell lines detected by two-dimensional gel electrophoresis

2015 ◽  
Vol 36 (4) ◽  
pp. 253-261 ◽  
Author(s):  
Yoshinori TAOKA ◽  
Kazumasa MATSUMOTO ◽  
Kazuya OHASHI ◽  
Satoru MINAMIDA ◽  
Masahiro HAGIWARA ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Protein Expression ◽  
Cell Lines ◽  
Cancer Cell ◽  
Expression Profile ◽  
Cisplatin Resistance ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Two Dimensional ◽  
Protein Expression Profile

scholarly journals Put Some Guts into It: Intestinal Organoid Models to Study Viral Infection

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1288 ◽  
Author(s):  
Inés García-Rodríguez ◽  
Adithya Sridhar ◽  
Dasja Pajkrt ◽  
Katja C. Wolthers
Keyword(s):  
Viral Infection ◽  
Cell Lines ◽  
Barrier Function ◽  
Three Dimensional ◽  
3D Models ◽  
Cellular Heterogeneity ◽  
Two Dimensional ◽  
Intestinal Organoids ◽  
Insight Into

The knowledge about enteric viral infection has vastly increased over the last eight years due to the development of intestinal organoids and enteroids that suppose a step forward from conventional studies using cell lines. Intestinal organoids and enteroids are three-dimensional (3D) models that closely mimic intestinal cellular heterogeneity and organization. The barrier function within these models has been adapted to facilitate viral studies. In this review, several adaptations (such as organoid-derived two-dimensional (2D) monolayers) and original intestinal 3D models are discussed. The specific advantages and applications, as well as improvements of each model are analyzed and an insight into the possible path for the field is given.

Characterization of Novel Cell Lines From Pleomorphic Adenomas of the Parotid Gland Established in a Collagen Gel System

1997 ◽  
Vol 107 (5) ◽  
pp. 654-660 ◽  
Author(s):  
Melissa G. Steiner ◽  
William I. Kuhel ◽  
John F. Carew ◽  
Jerry Huo ◽  
Syed A. Hoda ◽  
...  
Keyword(s):  
Parotid Gland ◽  
Cell Lines ◽  
Collagen Gel ◽  
Pleomorphic Adenomas ◽  
Gel System

scholarly journals Demonstration of a third structurally distinct human Ia beta chain by two-dimensional gel electrophoresis.

1982 ◽  
Vol 156 (2) ◽  
pp. 652-657 ◽  
Author(s):  
R W Karr ◽  
C C Kannapell ◽  
J A Stein ◽  
T C Fuller ◽  
R J Duquesnoy ◽  
...  
Keyword(s):  
Cell Lines ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Beta Chain ◽  
Two Dimensional Gel Electrophoresis ◽  
Hla Dr

Previous studies have indicated that HLA-DR homozygous cell lines express two Ia alpha and Ia beta chains that combine to form at least two Ia molecules. This report demonstrates by two-dimensional gel electrophoresis the existence of a third structurally distinct human Ia beta chain on DR2 and DR5 cell lines. This suggests that at least five separate genes control the expression of Ia molecules on HLA-DR homozygous cell lines.

scholarly journals A 41-kilodalton protein is a potential substrate for the p210bcr-abl protein-tyrosine kinase in chronic myelogenous leukemia cells.

1992 ◽  
Vol 12 (3) ◽  
pp. 1312-1323 ◽  
Author(s):  
E Freed ◽  
T Hunter
Keyword(s):  
Tyrosine Kinase ◽  
Cell Lines ◽  
Chronic Myelogenous Leukemia ◽  
Protein Tyrosine Kinase ◽  
Leukemia Virus ◽  
Lymphoid Cells ◽  
Myelogenous Leukemia ◽  
Two Dimensional ◽  
Kinase Gene ◽  
Protein Tyrosine

Chronic myelogenous leukemia (CML) is characterized by a translocation involving the c-abl protein-tyrosine kinase gene. A chimeric mRNA is formed containing sequences from a chromosome 22 gene (bcr) at its 5' end and all but the variable exon 1 of c-abl sequence. The product of this mRNA, p210bcr-abl, has constitutively high protein-tyrosine kinase activity. We examined K562 cells and other lines established from CML patients for the presence of phosphotyrosine (P-Tyr)-containing proteins which might be p210bcr-abl substrates. Two-dimensional gel separation of 32P-labeled proteins followed by phosphoamino acid analysis of 25 phosphoproteins, which comprised the major alkali-stable phosphoproteins, indicated that three related proteins of 41 kDa are the most prominent P-Tyr-containing proteins detected by this method. The 41-kDa phosphoproteins are found in two other CML lines that we examined but not in lines of similar lineage isolated from patients with distinct leukemic disease. A protein that comigrates with the major form of pp41 (pp41A) and contains P-Tyr is also found in murine fibroblasts and B-lymphoid cells transformed by Abelson murine leukemia virus, which encodes the v-abl protein, and in platelet-derived growth factor-treated fibroblasts, in which it has been described previously. We analyzed three pairs of Epstein-Barr virus-immortalized B-cell lines from individual CML patients and found that only the lines in which active p210bcr-abl was present contained detectable pp41. We also performed immunoblotting with anti-P-Tyr antibodies on the same CML cell lines and detected at least four other putative substrates of p210bcr-abl, which were undetected with use of the two-dimensional gel technique.

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