Transfer of Chinese hamster chromosome 1 to mouse cells and regional assignment of 7 genes: A combination of gene transfer and microcell fusion

1984 ◽  
Vol 10 (6) ◽  
pp. 547-559 ◽  
Author(s):  
Veena Dhar ◽  
Bernard M. Searle ◽  
Raghbir S. Athwal
Keyword(s):  
Gene Transfer ◽  
Chinese Hamster ◽  
Chromosome 1 ◽  
Mouse Cells ◽  
Microcell Fusion ◽  
Chinese Hamster Chromosome ◽  
Regional Assignment

scholarly journals Regional assignment of ENO1, PGD, and PGM1 to the long arm of Chinese hamster chromosome 2.

1985 ◽  
Vol 60 (3) ◽  
pp. 255-259
Author(s):  
Hitoshi SATOH ◽  
Shinichi SONTA ◽  
Michihiro C. YOSHIDA
Keyword(s):  
Chinese Hamster ◽  
Chromosome 2 ◽  
Chinese Hamster Chromosome ◽  
Regional Assignment

scholarly journals Regional assignment of the structural gene for human acid β-glucosidase to q42→qter on chromosome 1

1982 ◽  
Vol 33 (4) ◽  
pp. 340-344 ◽  
Author(s):  
E.A. Devine ◽  
M. Smith ◽  
F.X. Arredondo-Vega ◽  
B. Shafit-Zagardo ◽  
R.J. Desnick
Keyword(s):  
Structural Gene ◽  
Chromosome 1 ◽  
Human Acid ◽  
Beta Glucosidase ◽  
Regional Assignment

Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer

1989 ◽  
Vol 9 (4) ◽  
pp. 1754-1758
Author(s):  
T M Underhill ◽  
W F Flintoff
Keyword(s):  
Gene Transfer ◽  
Dna Sequences ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Wild Type ◽  
Ovary Cells ◽  
Ovary Cell Line ◽  
Human Specific

A methotrexate-resistant Chinese hamster ovary cell line deficient in methotrexate uptake has been complemented to methotrexate sensitivity by transfection with DNA isolated from either wild-type Chinese hamster ovary or human G2 cells. Primary and secondary transfectants regained the ability to take up methotrexate in a manner similar to that of wild-type cells, and in the case of those transfected with human DNA, to contain human-specific DNA sequences. The complementation by DNA-mediated gene transfer of this methotrexate-resistant phenotype provides a basis for the cloning of a gene involved in methotrexate uptake.

Microcell Fusion and Mammalian Gene Transfer

Gene Transfer ◽  
1986 ◽  
pp. 79-93 ◽  
Author(s):  
Tracy G. Lugo ◽  
R. E. K. Fournier
Keyword(s):  
Gene Transfer ◽  
Mammalian Gene ◽  
Microcell Fusion

Correction of Genetic Defects in Mammalian Cells by the Input of Small Amounts of Foreign Genetic Material

1973 ◽  
Vol 13 (3) ◽  
pp. 841-861
Author(s):  
YVONNE L. BOYD ◽  
H. HARRIS
Keyword(s):  
Thymidine Kinase ◽  
Mammalian Cells ◽  
Hybrid Cell ◽  
Genetic Material ◽  
Chinese Hamster ◽  
Heat Sensitivity ◽  
Chinese Hamster Cells ◽  
Inosinic Acid ◽  
Mouse Cells

Chinese hamster cells lacking inosinic acid pyrophosphorylase and mouse cells lacking thymidine kinase were fused with chick erythrocytes. The resultant heterokaryons were cultivated in a selective medium in which possession of these enzymes was essential for cell survival and growth. Clones of cells able to grow in this medium were isolated and studied. A detailed karyological analysis of these clones failed to reveal any chick chromosomes; nor could any chick-specific antigens be detected on the surface of the cells. Nonetheless, clones arising from the fusion of chick erythrocytes with Chinese hamster cells were shown to possess an inosinic acid pyrophosphorylase which had the electrophoretic characteristics of chick inosinic acid pyrophosphorylase. However, the clones arising from the fusion of the chick erythrocytes with the mouse cells had a thymidine kinase with the electrophoretic mobility and heat sensitivity of murine, not chick, thymidine kinase. Both types of hybrid cell have now been cultivated in vitro for 18 months without the loss of thymidine kinase or inosinic acid pyrophosphorylase activity.

Regional assignment of human amylase (AMY) to p22 ? p21 of chromosome 1

1984 ◽  
Vol 10 (2) ◽  
pp. 205-210 ◽  
Author(s):  
James V. Tricoli ◽  
Thomas B. Shows
Keyword(s):  
Chromosome 1 ◽  
Regional Assignment
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