The role of tube diameter and surface characteristics in the tolerance of roots to toxic solutions

1970 ◽  
Vol 33 (1-3) ◽  
pp. 235-243
Author(s):  
Earl Albregts ◽  
A. J. Ohlrogge
2014 ◽  
Vol 25 (2) ◽  
pp. 96-103 ◽  
Author(s):  
Camilla Christian Gomes Moura ◽  
Darceny Zanetta-Barbosa ◽  
Paula Dechichi ◽  
Valessa Florindo Carvalho ◽  
Priscilla Barbosa Ferreira Soares

Due to the critical role of monocytes/macrophages (Mϕ) in bone healing, this study evaluated the effects of bio-anodized, acid-etched, and machined titanium surfaces (Ti) on Mϕ behavior. Cells were separated from whole human blood from 10 patients, plated on Ti or polystyrene (control) surfaces, and cultured for 72 h. At 24, 48 and 72 h, cell viability, levels of IL1β, IL10, TNFα, TGFβ1 inflammatory mediators, and nitric oxide (NO) release were analyzed by mitochondrial colorimetric assay (MTT assay) and immunoenzymatic assays, respectively. Real-time PCR was used to verify the expression of TNFα and IL10 at 72 h. The data were subjected to a Kruskal-Wallis analysis. IL1β, TNFα and TGFβ1 release were not significantly different between the Ti surfaces (p>0.05). The presence of NO and IL10 was not detected in the samples. Cell viability did not differ between the samples cultivated on Ti and those cultivated on control surfaces, except at 24 h (p=0.0033). With respect to the mediators evaluated, the surface characteristics did not induce a typical Th1 or Th2 cytokine profile, although the cell morphology and topography were influenced by the Ti surface during the initial period.


2019 ◽  
Vol 37 (3) ◽  
pp. 3545-3553 ◽  
Author(s):  
Han Xu ◽  
Xiaocheng Mi ◽  
Charles B. Kiyanda ◽  
Hoi Dick Ng ◽  
John H.S. Lee ◽  
...  

2017 ◽  
Vol 58 (7) ◽  
pp. 1069-1075 ◽  
Author(s):  
Gilsang Hong ◽  
Junhyun Choi ◽  
Yosep Han ◽  
Kwang-Suk Yoo ◽  
Kwanho Kim ◽  
...  

2014 ◽  
Vol 28 (14) ◽  
pp. 5217-5229 ◽  
Author(s):  
An Liu ◽  
Dunzhu Li ◽  
Liang Liu ◽  
Yuntao Guan

Author(s):  
S J. Krause ◽  
W.W. Adams

It has been shown that there are numerous advantages in imaging both coated and uncoated polymers in scanning electron microscopy (SEM) at low voltages (LV) from 0.5 to 2.0 keV compared to imaging at conventional voltages of 10 to 20 keV. In imaging metal coated polymers in LVSEM beam damage is reduced, contrast is improved, and charging from irregularly shaped features is reduced or eliminated. Imaging uncoated polymers in LVSEM allows observation of the surface with little or no charging and with no alterations of surface features from the metal coating process required for higher voltage imaging. This is particularly important for high resolution studies where it is desired to image features 1 to 10 nm in size. At low voltages the sample-beam interaction volume resides closer to the surface which increases the ratio or exiting current to that of the primary beam current.


2011 ◽  
Vol 493-494 ◽  
pp. 866-871
Author(s):  
Anna Theocharidou ◽  
K. Tsoptsias ◽  
Eleana Kontonasaki ◽  
Lambrini Papadopoulou ◽  
C. Panayiotou ◽  
...  

Chitin is a polysaccharide abundant in nature. Its’ deacetylation product-chitosan- in combination with gelatin (collagen product) is commonly used asbiopolymer scaffold for tissue engineering. The aim of this study was to investigate diffrerences in surface characteristics of chitin (CHN CCS) and chitosan –gelatin (CHS-G CCS) composite ceramic scaffolds (CCS), during their incubation in culture medium (DMEM) with or without human periodontal ligament fibroblasts (HPDLF). CHN CCS and CHS- G CCS, with pore size 70-200μm, were fabricated on the surface of ceramic disks, being coated with a mixture of bioactive glass – ceramic (1:1 wt). Three CCSs of each type were constructed. Each CCS was incubated at 37 °C up to 10 days, either only in DMEM supplemented with 10% FCS or in DMEM with the presence of 105HPDLF. SEM microphotographs and EDS analysis, before and after incubation, were used to investigate CCSs’ surface alterations. Before incubation, all type of CCSs appeared to be macro porous with high interconnectivity. Exposed to incubation, CHN CCSs’ surface porosity seemed to be rapidly reduced and a rough surface without pores was observed with or without HPDLF. Attached HPDLF were rarely detected. CHS-G CCSs appeared to retain surface porosity in DMEM without cells. In HPDLF culture an almost uniform surface with organic aggregates and attached cells was observed. Until day 10, HPDLF could only be detected at CHS-G CCS’s surface. Conclusion: SEM microphotographs observations indicate that CHN CCSs’ incubation in DMEM led in early and rapid coalescence of surface pores, thus inhibiting HPDLF attachment. HPDLF attachment on CHS-G CCSs confirm the beneficial role of gelatin, while differences in CHS-G CCSs’ surface with and without HPDLF culture indicate that not only sedimentation of medium's ingredients, but cell attachment and function could decrease surface’s porosity, affecting consequently HPDLF proliferation.


Sign in / Sign up

Export Citation Format

Share Document