Radioimmunologic determination of plasma unconjugated estriol in normal and abnormal pregnancies with a specific antiserum to estriol

1974 ◽  
Vol 216 (4) ◽  
pp. 387-398 ◽  
Author(s):  
H. J. K�nzig ◽  
W. Geiger
Keyword(s):  
Specific Antiserum ◽  
Unconjugated Estriol

Determination of leukotriene C4 by radioimmunoassay with a specific antiserum generated from a synthetic hapten mimic

1984 ◽  
Vol 56 (11) ◽  
pp. 1862-1865 ◽  
Author(s):  
M. A. Wynalda ◽  
J. R. Brashler ◽  
M. K. Bach ◽  
D. R. Morton ◽  
F. A. Fitzpatrick
Keyword(s):  
Specific Antiserum ◽  
Leukotriene C4

Improved assay of unconjugated estriol in maternal serum or plasma by adsorption and liquid chromatography with fluorimetric detection.

1984 ◽  
Vol 30 (5) ◽  
pp. 742-744 ◽  
Author(s):  
F Andreolini ◽  
C Borra ◽  
A Di Corcia ◽  
A Laganà ◽  
R Samperi ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Maternal Serum ◽  
Graphitized Carbon Black ◽  
Fluorimetric Detection ◽  
Graphitized Carbon ◽  
Analytical Recovery ◽  
Chloroform Methanol ◽  
Unconjugated Estriol ◽  
In Pregnancy

Abstract In this improved assay only a 500-microL sample is needed and a single assay can be done within 30 min, 10 samples within 90 minutes. The sample of serum or plasma is diluted 20-fold with water, and the estriol is adsorbed from it onto graphitized carbon black ( Carbopack B, Supelco ). After two washings the estriol is desorbed with chloroform/methanol (60/40 by vol), which then is evaporated. The residue is redissolved in 50 microL of water/acetonitrile, and 20 microL is injected into the chromatograph. Analytical recovery for estriol-supplemented serum or plasma averaged 98.6%. Day-to-day CVs ranged from 3.9% at 2 micrograms/L to 2.1% at 20 micrograms/L. The limit of sensitivity is 0.3 micrograms/L, which makes this procedure suitable for determination of estriol even in the first half of pregnancy. Our method is inexpensive, and shows that liquid chromatography can be used to determine estriol in pregnancy serum or plasma. It also is more sensitive and precise and requires less sample than other such methods.

Nonchromatographic radioimmunoassay of unconjugated estriol in plasma, with polyethylene glycol as precipitant.

1976 ◽  
Vol 22 (3) ◽  
pp. 359-363 ◽  
Author(s):  
H S Schiller ◽  
M A Brammall
Keyword(s):  
Polyethylene Glycol ◽  
Clinical Laboratory ◽  
Specific Antiserum ◽  
Low Concentrations ◽  
Coefficients Of Variation ◽  
Free Steroid ◽  
Unconjugated Estriol

Abstract We describe a rapid, reliable radioimmunoassay for unconjugated estriol in plasma. Polyethylene glycol (Carbowax 6000) is used to separate antibody-bound and free steroid. The assay is sensitive (25 pg for standards), precise, and accurate. At high and low concentrations of estriol, intra-assay coefficients of variation were 7.1% and 7.6%, respectively, and inter-assay coefficients of variation were 7.2% and 10.0 %, respectively. Free [3H] estriol is not precipitated by polyethylene glycol. This radioimmunoassay of estriol, with a highly specific antiserum and with polyethylene glycol as the antibody precipitant, is a reliable one-day assay that is practical both for the clinical laboratory and the obstetrician.

Improved Techniques for Assessment of Plasma Lipoprotein Patterns. I. Precipitation in Gels after Electrophoresis with Polyanionic Compounds

1973 ◽  
Vol 19 (7) ◽  
pp. 737-739 ◽  
Author(s):  
Dietrich Seidel ◽  
Heinrich Wieland ◽  
Claudia Ruppert
Keyword(s):  
Differential Diagnosis ◽  
Plasma Lipoprotein ◽  
The Other ◽  
New Technique ◽  
Specific Antiserum ◽  
Fast Determination

Abstract A method is described for making lipoprotein fractions visible by polyanion precipitation in situ in the gels after electrophoresis. With the new technique the pattern and its interpretation in the differential diagnosis of all primary forms of hyperlipoproteinemia is the same as for the other lipid-staining procedures. The new technique is simpler to perform, reliable, and provides the required information 60 min after electrophoresis. It also allows a very fast determination of the abnormal lipoprotein (LP-X) that characterizes cholestasis, without use of a specific antiserum.

Variability in unconjugated and total estriol in serum during normal third trimester pregnancy.

1980 ◽  
Vol 26 (13) ◽  
pp. 1800-1803 ◽  
Author(s):  
L L Penney ◽  
W J Klenke
Keyword(s):  
Birth Weight ◽  
Third Trimester ◽  
The Third ◽  
Trimester Pregnancy ◽  
Data Support ◽  
Daily Data ◽  
Unconjugated Estriol

Abstract We determined unconjugated and total estriol concentrations in serum during the third trimester in 34 normal gestations. Data were obtained weekly for 13 women for as long as nine weeks; 21 others were studied daily for up to 15 days. No correlation between birth weight and either estriol fraction was demonstrated. Between-patient variability was less for unconjugated estriol and was similar for weekly and daily data. Within-patient variability was also less for unconjugated estriol and, as expected, was less for daily than for weekly data. The CV for daily samples, within patient, averaged 13.0% for unconjugated estriol and 20.3% for total estriol. Our data support the daily determination of unconjugated estriol in serum, evaluated for percent changes from single or mean preceding values, as the preferred method of monitoring estriol during pregnancy.

scholarly journals Immunochemical studies with a specific antiserum to rabbit fibroblast collagenase

1975 ◽  
Vol 151 (3) ◽  
pp. 655-663 ◽  
Author(s):  
Z Werb ◽  
J J Reynolds
Keyword(s):  
Immunoglobulin G ◽  
Synovial Fibroblast ◽  
Potent Inhibitor ◽  
Synovial Fibroblasts ◽  
Specific Antiserum ◽  
Collagenase Activity ◽  
Step Procedure ◽  
Α2 Macroglobulin ◽  
Fibroblast Collagenase

1. Antisera were raised against the collagenase from rabbit synovial fibroblasts and characterized by immunoprecipitation and immunoinhibition reactions. 2. Immunoglobulins from the antisera were potent inhibitors of the action of rabbit collagenase on both reconstituted collagen fibrils and collagen in solution. 3. The antibody-binding fragment, Fab′, produced by digesting the IgG (immunoglobulin G) with pepsin, inhibited collagenase activity just as well as whole IgG. 4. A specific antiserum to the rabbit collagenase was raised by a multi-step procedure. An initial antiserum was made by injecting partially purified collagenase as a complex with sheep α2-macroglobulin into a sheep. The non-specific antiserum so obtained was used to produce precipitin lines with the purified enzyme, and these lines were used as antigen for the production of the specific antiserum. 5. An IgG preparation from the specific antiserum was a specific and potent inhibitor of the rabbit synovial fibroblast collagenase. Neutral metallo-proteinase activity secreted by the rabbit fibroblasts was not inhibited by the antibody to the rabbit collagenase. 6. Criteria for determination of the specificity of antisera are discussed.

Galactic orbits of stars

1966 ◽  
Vol 25 ◽  
pp. 93-97
Author(s):  
Richard Woolley
Keyword(s):  
Globular Cluster ◽  
Potential Field ◽  
High Velocity ◽  
Velocity Vector ◽  
Variable Stars ◽  
The Sun ◽  
Proper Motions ◽  
Rr Lyrae ◽  
The Galaxy

It is now possible to determine proper motions of high-velocity objects in such a way as to obtain with some accuracy the velocity vector relevant to the Sun. If a potential field of the Galaxy is assumed, one can compute an actual orbit. A determination of the velocity of the globular clusterωCentauri has recently been completed at Greenwich, and it is found that the orbit is strongly retrograde in the Galaxy. Similar calculations may be made, though with less certainty, in the case of RR Lyrae variable stars.

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