scholarly journals Immunochemical studies with a specific antiserum to rabbit fibroblast collagenase

1975 ◽  
Vol 151 (3) ◽  
pp. 655-663 ◽  
Author(s):  
Z Werb ◽  
J J Reynolds
Keyword(s):  
Immunoglobulin G ◽  
Synovial Fibroblast ◽  
Potent Inhibitor ◽  
Synovial Fibroblasts ◽  
Specific Antiserum ◽  
Collagenase Activity ◽  
Step Procedure ◽  
Α2 Macroglobulin ◽  
Fibroblast Collagenase

1. Antisera were raised against the collagenase from rabbit synovial fibroblasts and characterized by immunoprecipitation and immunoinhibition reactions. 2. Immunoglobulins from the antisera were potent inhibitors of the action of rabbit collagenase on both reconstituted collagen fibrils and collagen in solution. 3. The antibody-binding fragment, Fab′, produced by digesting the IgG (immunoglobulin G) with pepsin, inhibited collagenase activity just as well as whole IgG. 4. A specific antiserum to the rabbit collagenase was raised by a multi-step procedure. An initial antiserum was made by injecting partially purified collagenase as a complex with sheep α2-macroglobulin into a sheep. The non-specific antiserum so obtained was used to produce precipitin lines with the purified enzyme, and these lines were used as antigen for the production of the specific antiserum. 5. An IgG preparation from the specific antiserum was a specific and potent inhibitor of the rabbit synovial fibroblast collagenase. Neutral metallo-proteinase activity secreted by the rabbit fibroblasts was not inhibited by the antibody to the rabbit collagenase. 6. Criteria for determination of the specificity of antisera are discussed.

scholarly journals Managing pH of Organic Matrices and New Commercial Substrates for Ornamental Plant Production: A Methodological Approach

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 851
Author(s):  
Sonia Cacini ◽  
Sara Di Lonardo ◽  
Simone Orsenigo ◽  
Daniele Massa
Keyword(s):  
Methodological Approach ◽  
Buffering Capacity ◽  
Ornamental Plant ◽  
Plant Production ◽  
Climatic Chamber ◽  
Step Procedure ◽  
Coir Dust ◽  
Organic Matrices ◽  
Substrate Mixtures

Professional peat-free substrates for ornamental plant production are increasingly required by nursery growers. Most promising materials are green compost, coconut coir dust, and woody fibre, used alone or in mixtures. One of the major concerns is pH, usually higher than optimal. In this work, a method based on a three-step procedure was adopted to acidify three organic matrices alone or in mixtures and to individuate the most suitable product, between iron(II) sulphate 7-hydrate and elemental sulphur chips. Firstly, the determination of the buffering capacity by dilution with sulphuric acid was carried out to determine dosages. Afterwards, an incubation trial of 84 (iron(II) sulphate) or 120 days (sulphur chips) was conducted on matrices and substrate mixtures with calculated doses in a climatic chamber maintained at 21 °C. Iron(II) sulphate resulted not suitable because it caused a rapid, but not lasting, pH lowering and an excessive electrical conductivity (EC) increase. Sulphur chips could instead guarantee an adequate and lasting pH lowering. These results were then validated in the open field trial on matrices and substrates. The proposed acidification methodology could be considered in developing new substrates, but the rapidity of pH acidification and EC increase on plant and mineral nutrition should be further investigated.

scholarly journals An Unprecedented High Content of the Bioactive Flavone Tricin in Huperzia Medicinal Species Used by the Saraguro in Ecuador

2016 ◽  
Vol 11 (3) ◽  
pp. 1934578X1601100
Author(s):  
Chabaco Armijos ◽  
Jorge Ponce ◽  
Jorge Ramírez ◽  
Davide Gozzini ◽  
Paola Vita Finzi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Traditional Medicine ◽  
Cell Lines ◽  
Cancer Cell ◽  
Potent Inhibitor ◽  
Community Living ◽  
Medicinal Species ◽  
Chemopreventive Agent ◽  
Southern Ecuador

The flavone tricin (5,7,4′-trihydroxy-3′,5′-dimethoxyflavone) is considered to be a selective potent inhibitor of different cancer cell lines and a potential colorectal cancer chemopreventive agent. In this paper we describe a reliable UHPLC-UV-ESIMS method for the determination of tricin in Huperzia plants used in the traditional medicine of the Saraguro community living in Southern Ecuador. An unusually high amount of tricin was found in H. brevifolia and H. compacta, which exceeded the content of this flavone determined so far in other plants.

Determination of leukotriene C4 by radioimmunoassay with a specific antiserum generated from a synthetic hapten mimic

1984 ◽  
Vol 56 (11) ◽  
pp. 1862-1865 ◽  
Author(s):  
M. A. Wynalda ◽  
J. R. Brashler ◽  
M. K. Bach ◽  
D. R. Morton ◽  
F. A. Fitzpatrick
Keyword(s):  
Specific Antiserum ◽  
Leukotriene C4

DESCRIPTION OF CHITOSANE GRAFTED ACRYLIC ACID BASED HYDROGEL SWELLING KINETICS AND THEIR USE IN NEW DESIGNED SYSTEM

2021 ◽  
Author(s):  
Tamara Erceg ◽  
◽  
Gaja Brakus ◽  
Dejan Kojić ◽  
Nevena Vukić ◽  
...  
Keyword(s):  
Acrylic Acid ◽  
Room Temperature ◽  
Cost Effective ◽  
Swelling Kinetics ◽  
Swelling Ratio ◽  
Empirical Dependence ◽  
Step Procedure ◽  
Dynamic Swelling ◽  
Hydrogel Swelling

Chitosan based hydrogel was synthesized via free-radical polymerization in a two-step procedure, after modification of chitosan by acrylic acid. Dynamic swelling test was carried out in distilled water at room temperature. Swelling kinetics was modeling using the principle of assuming the equation for a swelling ratio-time empirical dependence. Using regression analysis in this way gives the possibility for simpler determination of swelling ratio-time empirical dependence, knowing the graph functions. Obtained hydrogel was used for preparation of new bilayer hydrogel system which has potential application in wound dressing systems and other fields where pH sensitivity and improved mechanical properties of biopolymers are required in framework of green, cost-effective process.

Reliability-Based Criteria for the Decision of the Compensation of Corroded Flexural Reinforcement

2016 ◽  
pp. 364-380
Author(s):  
Ashraf Ragab Mohamed
Keyword(s):  
Carrying Capacity ◽  
Material Properties ◽  
Reliability Index ◽  
Basic Material ◽  
Uniform Corrosion ◽  
Step Procedure ◽  
Load Carrying ◽  
Flexural Reinforcement ◽  
Current Loading

Corrosion of reinforcement is considered as the major cause of most deteriorated concrete structures. As reinforcement corrodes, the load carrying capacity is affected and hence, the probability of failure increases. At the time of inspection of deteriorated structures, engineers are faced with the problem whether the available steel is enough to secure the safety of the structural member. This chapter addresses this problem based on reliability-based approach to evaluate the safety of the deteriorated members due to uniform corrosion under bending. A methodology is proposed to facilitate the determination of the member reliability index based on basic material properties and current loading. A step-by-step procedure is proposed based on charts developed in this study according to the Egyptian code provisions.

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