Nuclear size, DNA content, and chromatin condensation are different in individual tissues of the maize root apex

PROTOPLASMA ◽  
1990 ◽  
Vol 158 (1-2) ◽  
pp. 45-52 ◽  
Author(s):  
F. Baluška
Keyword(s):  
Dna Content ◽  
Chromatin Condensation ◽  
Root Apex ◽  
Nuclear Size ◽  
Maize Root

DNA in rat hepatocyte nuclei: effects of treatment with low levels of carbon tetrachloride and (or) chlordane

1979 ◽  
Vol 57 (5) ◽  
pp. 1003-1009 ◽  
Author(s):  
D. C. Mahon ◽  
K. K. Nair ◽  
P. C. Oloffs
Keyword(s):  
Liver Cirrhosis ◽  
Carbon Tetrachloride ◽  
Dna Content ◽  
Hepatic Dysfunction ◽  
Chromatin Condensation ◽  
Nuclear Size ◽  
Rat Hepatocyte ◽  
Highly Sensitive ◽  
Dna Contents ◽  
Low Levels

The effects of low-level, chronic feeding of the insecticide chlordane on the cytochemistry of the hepatocyte nuclei of male nits, with or without liver cirrhosis, were determined. Chlordane was given orally at 0.1 mg kg−1 day−1. Cirrhosis was induced by exposure of the animals to carbon tetrachloride (CCl4) vapours twice weekly for 10 weeks. The animals were killed after 20 weeks and the Feulgen-stained hepatocyte nuclei examined microspectrophotometrically for nuclear size, DNA content, and the degree of chromatin condensation. The five experimental groups were: (A) control; (B) chlordane only for 20 weeks; (C) cirrhosis only (CCl4 weeks 0–10); (D) chlordane (weeks 0–20) plus cirrhosis; (E) cirrhosis (CCl4, weeks 0–10) followed by chlordane (weeks 10–20). All treatments significantly (p < 0.01) increased nuclear size. Although DNA contents were not affected by chlordane alone, in cirrhotic animals treatment with chlordane caused significantly higher DNA contents than that in cirrhotics not receiving chlordane. This suggests that hepatic dysfunction increases the liver's sensitivity to respond to chlordane. Chromatin condensation was decreased by chlordane in both healthy and cirrhotic animals; animals with cirrhosis, but not receiving chlordane, had more condensed chromatin than the controls.The results of this study show that computer analysis of images of Feulgen-stained nuclei is a highly sensitive technique to detect the effects of chemicals on the degree of chromatin condensation even at dosage levels below the no-effect levels previously reported.

scholarly journals Mechanical phenotyping of acute promyelocytic leukemia reveals unique biomechanical responses in retinoic acid-resistant populations

2021 ◽  
Author(s):  
Brian Li ◽  
Annie Maslan ◽  
Aaron M Streets ◽  
Lydia L. Sohn
Keyword(s):  
Retinoic Acid ◽  
Acute Promyelocytic Leukemia ◽  
Dna Content ◽  
Trichostatin A ◽  
Chromatin Condensation ◽  
Promyelocytic Leukemia ◽  
Mechanical Responses ◽  
Nuclear Mechanics ◽  
All Trans Retinoic Acid ◽  
M Phase

While all-trans retinoic acid (ATRA) is an essential therapy in the treatment of acute promyelocytic leukemia (APL), an aggressive subtype of acute myeloid leukemia, nearly 20% of APL patients are resistant to ATRA. As no biomarkers for ATRA resistance yet exist, we investigated whether cell mechanics could be associated with this pathological phenotype. Using mechano-node-pore sensing, a single-cell mechanical phenotyping platform, and patient-derived APL cell lines, NB4 (ATRA-sensitive) and AP-1060 (ATRA-resistant), we discovered that ATRA-resistant APL cells are less mechanically pliable. By investigating how different subcellular components of APL cells contribute to whole-cell mechanical phenotype, we determined that nuclear mechanics strongly influence APL cell mechanical responses. By arresting APL cells in S-phase or M-phase in the cell cycle, we found cell pliability to be inversely related to DNA content. In addition to DNA content affecting cell pliability, we observed that chromatin condensation also affects nuclear mechanics: decondensing chromatin with trichostatin A is especially effective in softening ATRA-resistant APL cells. RNA-Seq allowed us to compare the transcriptomic differences between ATRA-resistant and ATRA-responsive APL cells and highlighted gene expression changes that could be associated with mechanical changes. Overall, we demonstrate the potential of physical biomarkers in identifying APL resistance.

scholarly journals Nitrate sensing by the maize root apex transition zone: a merged transcriptomic and proteomic survey

2015 ◽  
Vol 66 (13) ◽  
pp. 3699-3715 ◽  
Author(s):  
Sara Trevisan ◽  
Alessandro Manoli ◽  
Laura Ravazzolo ◽  
Alessandro Botton ◽  
Micaela Pivato ◽  
...  
Keyword(s):  
Transition Zone ◽  
Root Apex ◽  
Maize Root

scholarly journals Localization of aluminium in the maize root apex: can morin detect cell wall-bound aluminium?

2005 ◽  
Vol 56 (415) ◽  
pp. 1351-1357 ◽  
Author(s):  
Dejene Eticha ◽  
Angelika Staß ◽  
Walter J. Horst
Keyword(s):  
Cell Wall ◽  
Root Apex ◽  
Maize Root

Spontaneous evolution of nuclear size and DNA content of human non-small-cell-lung cancers grafted onto nude mice

Cytometry ◽  
1992 ◽  
Vol 13 (6) ◽  
pp. 586-594 ◽  
Author(s):  
Anna Kruczynski ◽  
Olivier Pauwels ◽  
Michel Wright ◽  
Georges Delsol ◽  
Robert Kiss
Keyword(s):  
Nude Mice ◽  
Dna Content ◽  
Small Cell ◽  
Nuclear Size ◽  
Small Cell Lung ◽  
Lung Cancers ◽  
Spontaneous Evolution

scholarly journals The Electrical Network of Maize Root Apex is Gravity Dependent

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Elisa Masi ◽  
Marzena Ciszak ◽  
Diego Comparini ◽  
Emanuela Monetti ◽  
Camilla Pandolfi ◽  
...  
Keyword(s):  
Root Apex ◽  
Maize Root ◽  
Electrical Network
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