scholarly journals Localization of aluminium in the maize root apex: can morin detect cell wall-bound aluminium?

2005 ◽  
Vol 56 (415) ◽  
pp. 1351-1357 ◽  
Author(s):  
Dejene Eticha ◽  
Angelika Staß ◽  
Walter J. Horst
Keyword(s):  
Cell Wall ◽  
Root Apex ◽  
Maize Root

scholarly journals Inhibition of cell expansion enhances cortical microtubule stability in the root apex of Arabidopsis thaliana

2021 ◽  
Vol 28 (1) ◽  
Author(s):  
Veronica Giourieva ◽  
Emmanuel Panteris
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Cell Expansion ◽  
Cortical Microtubule ◽  
Root Apex ◽  
Cellulose Synthase ◽  
Cellulose Biosynthesis ◽  
Cortical Microtubules ◽  
Wild Type ◽  
Microtubule Stability

Abstract Background Cortical microtubules regulate cell expansion by determining cellulose microfibril orientation in the root apex of Arabidopsis thaliana. While the regulation of cell wall properties by cortical microtubules is well studied, the data on the influence of cell wall to cortical microtubule organization and stability remain scarce. Studies on cellulose biosynthesis mutants revealed that cortical microtubules depend on Cellulose Synthase A (CESA) function and/or cell expansion. Furthermore, it has been reported that cortical microtubules in cellulose-deficient mutants are hypersensitive to oryzalin. In this work, the persistence of cortical microtubules against anti-microtubule treatment was thoroughly studied in the roots of several cesa mutants, namely thanatos, mre1, any1, prc1-1 and rsw1, and the Cellulose Synthase Interacting 1 protein (csi1) mutant pom2-4. In addition, various treatments with drugs affecting cell expansion were performed on wild-type roots. Whole mount tubulin immunolabeling was applied in the above roots and observations were performed by confocal microscopy. Results Cortical microtubules in all mutants showed statistically significant increased persistence against anti-microtubule drugs, compared to those of the wild-type. Furthermore, to examine if the enhanced stability of cortical microtubules was due to reduced cellulose biosynthesis or to suppression of cell expansion, treatments of wild-type roots with 2,6-dichlorobenzonitrile (DCB) and Congo red were performed. After these treatments, cortical microtubules appeared more resistant to oryzalin, than in the control. Conclusions According to these findings, it may be concluded that inhibition of cell expansion, irrespective of the cause, results in increased microtubule stability in A. thaliana root. In addition, cell expansion does not only rely on cortical microtubule orientation but also plays a regulatory role in microtubule dynamics, as well. Various hypotheses may explain the increased cortical microtubule stability under decreased cell expansion such as the role of cell wall sensors and the presence of less dynamic cortical microtubules.

Cytological and histochemical characteristics of the axenic root surface of Alnus glutinosa

1986 ◽  
Vol 64 (10) ◽  
pp. 2216-2226 ◽  
Author(s):  
Yves Prin ◽  
Mireille Rougier
Keyword(s):  
Cell Wall ◽  
Root Hair ◽  
Root Hairs ◽  
Root Apex ◽  
Alnus Glutinosa ◽  
Root Surface ◽  
Infection Process ◽  
Root Cap ◽  
A Cell ◽  
Histochemical Tests

The aim of the present study was to investigate the Alnus root surface using seedlings grown axenically. This study has focused on root zones where infection by the symbiotic actinomycete Frankia takes place. The zones examined extend from the root cap to the emerging root hair zone. The root cap ensheaths the Alnus root apex and extends over the root surface as a layer of highly flattened cells closely appressed to the root epidermal cell wall. These cells contain phenolic compounds as demonstrated by various histochemical tests. They are externally bordered by a thin cell wall coated by a thin mucilage layer. The root cap is ruptured when underlying epidermal cells elongate, and cell remnants are still found in the emerging root hair zone. Young emerging root hairs are bordered externally by a cell wall covered by a thin mucilage layer which reacts positively to the tests used for the detection of polysaccharides, glycoproteins, and anionic sites. The characteristics of the Alnus root surface and the biological function of mucilage and phenols present at the root surface are discussed in relation to the infection process.

scholarly journals The Endoplasmic Reticulum and the Golgi Structures in Maize Root Cells

1959 ◽  
Vol 5 (3) ◽  
pp. 501-506 ◽  
Author(s):  
W. Gordon Whaley ◽  
Hilton H. Mollenhauer ◽  
Joyce E. Kephart
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Wall ◽  
Electron Microscope ◽  
Nuclear Envelope ◽  
Epoxy Resin ◽  
Maize Root ◽  
Root Tips ◽  
Animal Cells ◽  
Root Cells ◽  
Vesicular Structure

Maize root tips were fixed in potassium permanganate, embedded in epoxy resin, sectioned to show silver interference color, and studied with the electron microscope. All the cells were seen to contain an endoplasmic reticulum and apparently independent Golgi structures. The endoplasmic reticulum is demonstrated as a membrane-bounded, vesicular structure comparable in many aspects to that of several types of animal cells. With the treatment used here the membranes appear smooth surfaced. The endoplasmic reticulum is continuous with the nuclear envelope and, by contact at least, with structures passing through the cell wall. The nuclear envelope is characterized by discontinuities, as previously reported for animal cells. The reticula of adjacent cells seem to be in contact at or through the plasmodesmata. Because of these contacts the endoplasmic reticulum of a given cell appears to be part of an intercellular system. The Golgi structures appear as stacks of platelet-vesicles which apparently may, under certain conditions, produce small vesicles around their edges. Their form changes markedly with development of the cell.

scholarly journals Nitrate sensing by the maize root apex transition zone: a merged transcriptomic and proteomic survey

2015 ◽  
Vol 66 (13) ◽  
pp. 3699-3715 ◽  
Author(s):  
Sara Trevisan ◽  
Alessandro Manoli ◽  
Laura Ravazzolo ◽  
Alessandro Botton ◽  
Micaela Pivato ◽  
...  
Keyword(s):  
Transition Zone ◽  
Root Apex ◽  
Maize Root

scholarly journals The Electrical Network of Maize Root Apex is Gravity Dependent

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Elisa Masi ◽  
Marzena Ciszak ◽  
Diego Comparini ◽  
Emanuela Monetti ◽  
Camilla Pandolfi ◽  
...  
Keyword(s):  
Root Apex ◽  
Maize Root ◽  
Electrical Network

Postmitotic ‘isodiametric’ cell growth in the maize root apex

Planta ◽  
1990 ◽  
Vol 181 (3) ◽  
pp. 269-274 ◽  
Author(s):  
F. Baluška ◽  
Š. Kubica ◽  
M. Hauskrecht
Keyword(s):  
Cell Growth ◽  
Root Apex ◽  
Maize Root
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