Classification of porcine enteroviruses by antigenic analysis and cytopathic effects in tissue culture: Description of 3 new serotypes

1979 ◽  
Vol 62 (3) ◽  
pp. 201-208 ◽  
Author(s):  
N. J. Knowles ◽  
L. S. Buckley ◽  
H. G. Pereira
Keyword(s):  
Tissue Culture ◽  
Antigenic Analysis ◽  
Cytopathic Effects

scholarly journals Virus-Like Particles and Cytopathic Activity in Urine of Patients with Leukemia

Blood ◽  
1966 ◽  
Vol 28 (3) ◽  
pp. 465-478 ◽  
Author(s):  
RICHARD P. AMES ◽  
JOSEPH T. SOBOTA ◽  
REGINALD L. REAGAN ◽  
MYRON KARON
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Acute Leukemia ◽  
Vitro Assay ◽  
Chronic Granulocytic Leukemia ◽  
Virus Like Particles ◽  
Cytopathic Effects ◽  
Culture Activity ◽  
Granulocytic Leukemia

Abstract Urine pellet material of patients with leukemia was examined by electron microscopy in an effort to find more convenient sources of virus material for tissue culture and animal infectivity studies. Thirty urine specimens from 16 patients with acute leukemia, 2 patients with chronic granulocytic leukemia and 1 patient with Burkitt’s lymphoma, and 17 urine samples from 15 control patients were examined. Virus-like particles were observed in 7 of the 16 patients with acute leukemia, 1 of the 2 patients with chronic granulocytic leukemia, and 1 of the 15 controls. Although these particles showed slight morphologic differences, many were similar in ultrastructure and in density gradient characteristics to the virus particles observed in murine leukemia and to the particles seen by other workers in plasma and tissues of patients with leukemia and lymphoma. Cytopathic effects on WI-38 fibroblasts were observed in urine from 3 patients with leukemia. These effects resembled cytomegalovirus infection, but confirmatory tests were not carried out. Whether the virus-like particles demonstrated by electron microscopy in this study are responsible for the cytopathic effects is at present uncertain. The correlation of a morphologic structure with tissue culture activity cannot be answered until suitable in vitro assay and transformation systems are developed to purify and test these particles. This study suggests that urine may serve as a convenient source for the study and development of such test systems using the electron microscope to correlate structure with biologic activity.

Growth and cytopathic effects of rubella virus in primary rabbit tissue culture

1965 ◽  
Vol 16 (1-5) ◽  
pp. 415-418 ◽  
Author(s):  
K. McCarthy ◽  
C. H. Taylor-Robinson
Keyword(s):  
Tissue Culture ◽  
Rubella Virus ◽  
Cytopathic Effects ◽  
Rabbit Tissue ◽  
Primary Rabbit

Classification of Tissue Culture Segments by Colour Machine Vision

1993 ◽  
Vol 55 (4) ◽  
pp. 299-311 ◽  
Author(s):  
V. Alchanatis ◽  
K. Peleg ◽  
M. Ziv
Keyword(s):  
Tissue Culture ◽  
Machine Vision

scholarly journals LATENT VIRAL INFECTION OF CELLS IN TISSUE CULTURE

1961 ◽  
Vol 113 (2) ◽  
pp. 271-281 ◽  
Author(s):  
John P. Bader ◽  
Herbert R. Morgan
Keyword(s):  
Tissue Culture ◽  
Folic Acid ◽  
Cell Cultures ◽  
Salt Solution ◽  
Virus Production ◽  
Water Soluble ◽  
Maximal Growth ◽  
Cytopathic Effects ◽  
Latent Viral Infection ◽  
Vitamin Requirement

A study of the metabolic requirements for the growth of psittacosis virus in L cells has been extended to the water-soluble vitamins. In a system in which a balanced salt solution was used to deplete the cells of their vitamin constituents, only thiamine was essential for psittacosis virus production. Extended depletion of cells with media deficient in specific vitamins demonstrated that pantothenate, niacin (niacinamide), pyridoxine (pyridoxal), and choline, in addition to thiamine, were essential for maximal growth of psittacosis virus. No requirement for biotin, inositol, folic acid, or riboflavin was demonstrated, although the possibility of incomplete vitamin depletion of the cells has not been eliminated. In most cases in which a specific vitamin requirement was shown the decreased yield of virus was correlated with a delay in the cytopathic effects produced in the cell cultures by psittacosis virus.

Note on the spectral classification of carbon stars in the photographic infrared region

1966 ◽  
Vol 24 ◽  
pp. 21-23
Author(s):  
Y. Fujita
Keyword(s):  
Infrared Region ◽  
Spectral Classification ◽  
Carbon Stars

We have investigated the spectrograms (dispersion: 8Å/mm) in the photographic infrared region fromλ7500 toλ9000 of some carbon stars obtained by the coudé spectrograph of the 74-inch reflector attached to the Okayama Astrophysical Observatory. The names of the stars investigated are listed in Table 1.

Tissue section lifting for electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 86-87
Author(s):  
Adrian F. van Dellen
Keyword(s):  
Infectious Disease ◽  
Electron Microscopy ◽  
Tissue Culture ◽  
Organ System ◽  
Surrounding Tissue ◽  
Paraffin Sections ◽  
Surface Areas ◽  
Specific Determination ◽  
High Degree ◽  
Accuracy And Repeatability

The morphologic pathologist may require information on the ultrastructure of a non-specific lesion seen under the light microscope before he can make a specific determination. Such lesions, when caused by infectious disease agents, may be sparsely distributed in any organ system. Tissue culture systems, too, may only have widely dispersed foci suitable for ultrastructural study. In these situations, when only a few, small foci in large tissue areas are useful for electron microscopy, it is advantageous to employ a methodology which rapidly selects a single tissue focus that is expected to yield beneficial ultrastructural data from amongst the surrounding tissue. This is in essence what "LIFTING" accomplishes. We have developed LIFTING to a high degree of accuracy and repeatability utilizing the Microlift (Fig 1), and have successfully applied it to tissue culture monolayers, histologic paraffin sections, and tissue blocks with large surface areas that had been initially fixed for either light or electron microscopy.

Diagnostic Value of Electron Microscopy in Soft Tissue Tumors

1970 ◽  
Vol 28 ◽  
pp. 220-221
Author(s):  
Gerald Fine ◽  
Azorides R. Morales
Keyword(s):  
Cardiac Myxoma ◽  
Osteogenic Sarcoma ◽  
Diagnostic Value ◽  
Soft Tissue Tumors ◽  
Amelanotic Melanoma ◽  
Growth Patterns ◽  
Light Microscopic Level ◽  
Mesenchymal Tumors ◽  
Good Agreement

For years the separation of carcinoma and sarcoma and the subclassification of sarcomas has been based on the appearance of the tumor cells and their microscopic growth pattern and information derived from certain histochemical and special stains. Although this method of study has produced good agreement among pathologists in the separation of carcinoma from sarcoma, it has given less uniform results in the subclassification of sarcomas. There remain examples of neoplasms of different histogenesis, the classification of which is questionable because of similar cytologic and growth patterns at the light microscopic level; i.e. amelanotic melanoma versus carcinoma and occasionally sarcoma, sarcomas with an epithelial pattern of growth simulating carcinoma, histologically similar mesenchymal tumors of different histogenesis (histiocytoma versus rhabdomyosarcoma, lytic osteogenic sarcoma versus rhabdomyosarcoma), and myxomatous mesenchymal tumors of diverse histogenesis (myxoid rhabdo and liposarcomas, cardiac myxoma, myxoid neurofibroma, etc.)

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