Comparison by restriction fragment pattern analysis and molecular characterization of some European isolates of Suid herpesvirus 1: a contribution to strain differentiation of European isolates

1988 ◽  
Vol 102 (1-2) ◽  
pp. 39-47 ◽  
Author(s):  
L. S. Christensen
Keyword(s):  
Molecular Characterization ◽  
Restriction Fragment ◽  
Pattern Analysis ◽  
Strain Differentiation ◽  
Fragment Pattern ◽  
Restriction Fragment Pattern ◽  
Herpesvirus 1

scholarly journals 16S Ribosomal DNA Terminal Restriction Fragment Pattern Analysis of Bacterial Communities in Feces of Rats Fed Lactobacillus acidophilus NCFM

2001 ◽  
Vol 67 (4) ◽  
pp. 1935-1939 ◽  
Author(s):  
Christopher W. Kaplan ◽  
Johanna C. Astaire ◽  
Mary Ellen Sanders ◽  
Bandaru S. Reddy ◽  
Christopher L. Kitts
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Lactobacillus Acidophilus ◽  
Bacterial Communities ◽  
Pattern Analysis ◽  
Bacterial Populations ◽  
Fragment Pattern ◽  
16S Ribosomal Dna ◽  
Restriction Fragment Pattern ◽  
Lactobacillus Acidophilus Ncfm

ABSTRACT 16S ribosomal DNA terminal restriction fragment patterns from rat fecal samples were analyzed to track the dynamics ofLactobacillus acidophilus NCFM and discern bacterial populations that changed during feeding with NCFM. Lactobacillus johnsonii and Ruminococcus flavefaciens were tentatively identified as such bacterial populations. The presence ofL. johnsonii was confirmed by isolation from feces.

scholarly journals Plasmid analysis ofSalmonella enteritidisisolated from human gastroenteritis cases and from epidemiologically associated poultry flocks

1993 ◽  
Vol 111 (2) ◽  
pp. 239-244 ◽  
Author(s):  
C. R. Dorn ◽  
R. Silapanuntakul ◽  
E. J. Angrick ◽  
L. D. Shipman
Keyword(s):  
Restriction Fragment ◽  
Salmonella Enteritidis ◽  
Plasmid Profile ◽  
Egg Laying ◽  
Internal Organs ◽  
Fragment Pattern ◽  
Plasmid Analysis ◽  
Commercial Flock ◽  
Restriction Fragment Pattern ◽  
Human Infections

SummaryPlasmid analysis ofSalmonella enteritidisisolates from human gastroenteritis cases and from two commercial egg-producing poultry flocks was performed to determine if the poultry flocks were the source of the human infections. The plasmid profile and restriction fragment pattern (fingerprint) of fiveS. enteritidisisolates from human cases matched those of nine isolates from internal organs of egg-laying hens in one flock which was the source of eggs consumed by the cases. Another commercial flock was epidemiologically associated as the source of eggs consumed by affected persons in four separate gastroenteritis outbreaks from whichS. enteritidisisolates were available. FiveS. enteritidisisolates from human cases in these four outbreaks had the same profile and fingerprint, and they all matched those of the 24 isolates from hens in this flock. These results provide further documentation of egg-borne transmission ofS. enteritidisto humans.

scholarly journals First Report on the Detection and Molecular Characterization of Bovine Herpesvirus 1 from a Clinical case of Infectious Bovine Rhinotracheitis in Pakistan

2021 ◽  
Vol 41 (01) ◽  
pp. 160-162
Author(s):  
Aayesha Riaz
Keyword(s):  
Molecular Characterization ◽  
Nested Pcr ◽  
Bovine Herpesvirus ◽  
Nasal Discharge ◽  
Infectious Bovine Rhinotracheitis ◽  
Bovine Herpesvirus 1 ◽  
Nasal Swabs ◽  
Herpesvirus 1 ◽  
First Time

Bovine herpesvirus 1 (BHV-1) is a noteworthy reason for many Cattle/Buffalo diseases. Infectious bovine rhinotracheitis (IBR) is one of the diseases which are caused by the BHV-1. In the present study a cow which was suspected of IBR was examined. The animal was suffering from fever and respiratory distress along with rhinitis (red nose), in appetence, and dyspnea. The nasal mucosa and muzzle were distinctly inflamed, with nasal discharge. DNA from blood samples and nasal swabs were subjected to nested PCR using glycoprotein B gene (gB) Primers. The samples were found positive for BHV-1 gB gene. Sequencing and phylogenetic analysis revealed close similarities with other BHV-1 gB gene sequences. The accession numbers assigned to this pioneer sequences in GenBank are MT449510 (BHV-1-Pak 1) and MT449511 (BHV-1-Pak 2). In this study, we reported for the first time the detection of DNA of BHV-1 through nested PCR assay and molecular characterization of BHV-1 gB gene in Pakistan. This study will be useful in further diagnoses of BHV-1 in Pakistan and in development of BHV-1 vaccine to reduce economical losses due to IBR

Sign in / Sign up

Export Citation Format

Share Document