Restriction fragment pattern (RFP) analysis of genomes from Danish isolates of suid herpesvirus 1 (Aujezsky's disease virus)

1987 ◽  
Vol 97 (3-4) ◽  
pp. 215-224 ◽  
Author(s):  
L. S. Christensen ◽  
K. J. Soerensen ◽  
J. C. Lei
Keyword(s):  
Disease Virus ◽  
Restriction Fragment ◽  
Fragment Pattern ◽  
Restriction Fragment Pattern ◽  
Herpesvirus 1

scholarly journals Plasmid analysis ofSalmonella enteritidisisolated from human gastroenteritis cases and from epidemiologically associated poultry flocks

1993 ◽  
Vol 111 (2) ◽  
pp. 239-244 ◽  
Author(s):  
C. R. Dorn ◽  
R. Silapanuntakul ◽  
E. J. Angrick ◽  
L. D. Shipman
Keyword(s):  
Restriction Fragment ◽  
Salmonella Enteritidis ◽  
Plasmid Profile ◽  
Egg Laying ◽  
Internal Organs ◽  
Fragment Pattern ◽  
Plasmid Analysis ◽  
Commercial Flock ◽  
Restriction Fragment Pattern ◽  
Human Infections

SummaryPlasmid analysis ofSalmonella enteritidisisolates from human gastroenteritis cases and from two commercial egg-producing poultry flocks was performed to determine if the poultry flocks were the source of the human infections. The plasmid profile and restriction fragment pattern (fingerprint) of fiveS. enteritidisisolates from human cases matched those of nine isolates from internal organs of egg-laying hens in one flock which was the source of eggs consumed by the cases. Another commercial flock was epidemiologically associated as the source of eggs consumed by affected persons in four separate gastroenteritis outbreaks from whichS. enteritidisisolates were available. FiveS. enteritidisisolates from human cases in these four outbreaks had the same profile and fingerprint, and they all matched those of the 24 isolates from hens in this flock. These results provide further documentation of egg-borne transmission ofS. enteritidisto humans.

scholarly journals 16S Ribosomal DNA Terminal Restriction Fragment Pattern Analysis of Bacterial Communities in Feces of Rats Fed Lactobacillus acidophilus NCFM

2001 ◽  
Vol 67 (4) ◽  
pp. 1935-1939 ◽  
Author(s):  
Christopher W. Kaplan ◽  
Johanna C. Astaire ◽  
Mary Ellen Sanders ◽  
Bandaru S. Reddy ◽  
Christopher L. Kitts
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Lactobacillus Acidophilus ◽  
Bacterial Communities ◽  
Pattern Analysis ◽  
Bacterial Populations ◽  
Fragment Pattern ◽  
16S Ribosomal Dna ◽  
Restriction Fragment Pattern ◽  
Lactobacillus Acidophilus Ncfm

ABSTRACT 16S ribosomal DNA terminal restriction fragment patterns from rat fecal samples were analyzed to track the dynamics ofLactobacillus acidophilus NCFM and discern bacterial populations that changed during feeding with NCFM. Lactobacillus johnsonii and Ruminococcus flavefaciens were tentatively identified as such bacterial populations. The presence ofL. johnsonii was confirmed by isolation from feces.

scholarly journals PCR-RFLP Analysis of D-Loop mtDNA in Indonesian Domestic Waterfowl

2017 ◽  
Vol 9 (3) ◽  
pp. 537
Author(s):  
R Susanti ◽  
Fidia Fibriana ◽  
Ari Yuniastuti
Keyword(s):  
Restriction Fragment ◽  
Rflp Analysis ◽  
Muscovy Duck ◽  
Genetic Characteristics ◽  
Fragment Pattern ◽  
Central Java ◽  
Pcr Rflp ◽  
Restriction Sites ◽  
D Loop ◽  
Restriction Fragment Pattern

<p class="IsiAbstrakIndo"><span lang="EN-GB">Phylogenetic or population genetic studies have been focused on finding genetic variations in mitochondrial DNA (mtDNA) because mutations in this region contained five times higher than other genetic materials. The aim of this research was to generate PCR-RFLP D-loop mtDNA profiles of some Indonesian domestic waterfowl. The samples were 35 local ducks, 5 muscovy duck, and 5 geese. These local ducks included on seven types of Javanese ducks in Central Java, i.e. Magelang duck, Peking duck, Pengging duck, Tegal Branjangan duck, Tegal Jarakan duck, Tegal Blorong duck, and Tegal Lemahan duck. PCR technique was used to amplify the D-loop genes, and RFLP analysis of the D-loop was performed with </span><em><span lang="EN-GB">Alu</span></em><span lang="EN-GB">I</span><em></em><span lang="EN-GB">and </span><em><span lang="EN-GB">Rsa</span></em><span lang="EN-GB">I. The results show that muscovy duck has no restriction sites in D-loop gene. Moreover, goose has a restriction </span><em><span lang="EN-GB">s</span></em><span lang="EN-GB">ite of </span><em><span lang="EN-GB">Alu</span></em><span lang="EN-GB">I in D-loop gene at 550 bp and 187 bp; and it has no restriction site of </span><em><span lang="EN-GB">Rsa</span></em><span lang="EN-GB">I. The analysis of </span><em><span lang="EN-GB">Rsa</span></em><span lang="EN-GB">I-RFLP in mtDNA </span><em><span lang="EN-GB">D-loop </span></em><span lang="EN-GB">region of ducks resulted in 1 restriction fragment pattern which can be applied in the identification of duck to differentiate it from goose and muscovy duck. PCR-RFLP analysis duck D-loop mtDNA using </span><em><span lang="EN-GB">Alu</span></em><span lang="EN-GB">I and </span><em><span lang="EN-GB">Rsa</span></em><span lang="EN-GB">I restriction enzyme resulted in 3 combinations of restriction fragment pattern shown in 3 haplotypes (A, B, and C). Genetic diversity of Indonesian domestic waterfowl population could be identified by using PCR-RFLP analysis on mtDNA </span><em><span lang="EN-GB">D-loop </span></em><span lang="EN-GB">region. So far, there are few results of research on Indonesian domestic waterfowl genetic characteristics based on PCR-RFLP mtDNA D-loop marker. Also, this research has a good contribution on genetic characterization of local duck. The characterization is an important aspect to maintain breed quality and manage the genetic resources of Indonesian germplasm and in facilitating the breeding program. </span><strong></strong></p>

scholarly journals Polymorphism and Linkage of the αA-Crystallin Gene in t-Haplotypes of the Mouse

Genetics ◽  
1987 ◽  
Vol 116 (1) ◽  
pp. 107-111
Author(s):  
Loren C Skow ◽  
Joseph N Nadeau ◽  
Jong Cheol Ahn ◽  
Hee-Sup Shin ◽  
Karen Artzt ◽  
...  
Keyword(s):  
Restriction Fragment ◽  
Inbred Strains ◽  
Distal Region ◽  
Wild Type ◽  
Inverted Region ◽  
Fragment Pattern ◽  
Inbred Strains Of Mice ◽  
Restriction Fragment Pattern ◽  
Phenotypic Groups ◽  
Recombinant Mice

ABSTRACT Restriction fragment polymorphisms were used to order the αA-crystallin locus (Crya-1) relative to other genes in mouse t-chromatin and to investigate the relatedness of α-A-crystallin sequences among different t-haplotypes. Analysis of DNA from t-recombinant mice mapped Crya-1 to the K end of the H-2 complex and within the distal inverted region characteristic of t-haplotypes. Hybridization with Crya-1 cDNA revealed three distinct phenotypic groups among the 17 different t-haplotypes studied. A majority (9 of 17) of the t-haplotypes were classified into a novel group (Crya-1t) characterized by restriction fragments apparently unique to t-chromosomes and therefore thought to contain αA-crystallin sequences descended from the original t-chromosome. A second group of t-haplotypes had restriction fragment patterns indistinguishable from those observed among many common inbred strains of mice of the Crya-1a type, and a third restriction fragment pattern, observed only in the tw121 haplotype, was indistinguishable from the fragment pattern for C3H/DiSn (Crya-1b) and several other inbred strains of mice. Thus, with respect to sequences around the Crya-1 locus, different t-haplotypes show restriction fragment polymorphisms, some of which are comparable to those found in wild-type chromosomes and provide further evidence for genetic heterogeneity in DNA from the distal region of t-haplotypes.

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