Infected cell specific protein and viral DNA synthesis in productive and abortive infections ofSpodoptera frugiperda nuclear polyhedrosis virus

1990 ◽  
Vol 115 (1-2) ◽  
pp. 101-113 ◽  
Author(s):  
Hsiao-Sheng Liu ◽  
S. L. Bilimoria
Keyword(s):  
Infected Cell ◽  
Dna Synthesis ◽  
Nuclear Polyhedrosis Virus ◽  
Specific Protein ◽  
Viral Dna ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

A VIRUS DISEASE OF ECTROPIS CREPUSCULARIA SCHIFF. (GEOMETRIDAE: LEPIDOPTERA)

1967 ◽  
Vol 13 (7) ◽  
pp. 855-858 ◽  
Author(s):  
Oswald N. Morris
Keyword(s):  
Dna Synthesis ◽  
Deoxyribonucleic Acid ◽  
Nuclear Polyhedrosis Virus ◽  
Virus Disease ◽  
Disease Process ◽  
Virus Diseases ◽  
Virus Particles ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

The morphology of a nuclear polyhedrosis virus of Ectropis crepuscularia Schiff., the histopathology of the disease, and deoxyribonucleic acid (DNA) synthesis during the disease process were studied. The polyhedral inclusions measure 1.4 μ in diameter with a range of 0.8–2.1 μ. The virus particles are rods measuring 319 mμ by 79 mμ with a range of 280–360 mμ by 70–110 mμ. DNA labelling and nuclear swelling follow the course of previously described polyhedrosis virus diseases.

scholarly journals Autographa californica Nuclear Polyhedrosis Virus DNA Polymerase: Measurements of Processivity and Strand Displacement

1999 ◽  
Vol 73 (6) ◽  
pp. 4908-4918 ◽  
Author(s):  
Vivien V. McDougal ◽  
Linda A. Guarino
Keyword(s):  
Dna Polymerase ◽  
Protein Interactions ◽  
Nuclear Polyhedrosis Virus ◽  
Recombinant Baculovirus ◽  
Specific Protein ◽  
Autographa Californica ◽  
Dependent Manner ◽  
Strand Displacement ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

ABSTRACT The DNA polymerase (DNApol) of Autographa californicanuclear polyhedrosis virus was purified to homogeneity from recombinant baculovirus-infected cells. DNApol was active in polymerase assays on singly primed M13 template, and full-length replicative form II product was synthesized at equimolar ratios of enzyme to template. The purified recombinant DNApol was shown to be processive by template challenge assay. Furthermore, DNApol was able to incorporate hundreds of nucleotides on an oligo(dT)-primed poly(dA) template with limiting amounts of polymerase. DNApol has moderate strand displacement activity, as it was active on nicked and gapped templates, and displaced a primer in a replication-dependent manner. Addition of saturating amounts of LEF-3, the viral single-stranded DNA-binding protein (SSB), increased the innate strand displacement ability of DNApol. However, when LEF-3 was added prior to the polymerase, it failed to stimulate DNApol replication on a singly primed M13 template because the helix-destabilizing activity of LEF-3 caused the primer to dissociate from the template. Escherichia coli SSB efficiently substituted for LEF-3 in the replication of a nicked template, suggesting that specific protein-protein interactions were not required for strand displacement in this assay.

Formation of nuclear polyhedral bodies and nuclear polyhedrosis virus of silkworm in mammalian cells infected with viral DNA

Virology ◽  
1967 ◽  
Vol 33 (3) ◽  
pp. 507-512 ◽  
Author(s):  
Michio Himeno ◽  
Fukumi Sakai ◽  
Konoshin Onodera ◽  
Hisao Nakai ◽  
Tetsuo Fukada ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Nuclear Polyhedrosis Virus ◽  
Viral Dna ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Polyhedral Bodies
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