Serial passage in tissue culture of mixed foot-and-mouth disease virus serotypes

1995 ◽  
Vol 140 (4) ◽  
pp. 783-787 ◽  
Author(s):  
E. L. Woodbury ◽  
A. R. Samuel ◽  
N. J. Knowles
Keyword(s):  
Tissue Culture ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Serial Passage ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Serial passage of foot-and-mouth disease virus in sheep reveals declining levels of viraemia over time

2002 ◽  
Vol 83 (8) ◽  
pp. 1907-1914 ◽  
Author(s):  
Gareth J. Hughes ◽  
Valerie Mioulet ◽  
Daniel T. Haydon ◽  
R. Paul Kitching ◽  
Alex I. Donaldson ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Infectious Agent ◽  
Serial Passage ◽  
Mouth Disease ◽  
Individual Values ◽  
Closed Population ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Serial Chains

If an infectious agent is to maintain itself within a closed population by means of an unbroken serial chain of infections, it must maintain the level of infectiousness of individuals through time, or termination of the transmission chain is inevitable. One possible cause of diminution in infectiousness along serial chains of transmission may be that individuals are unable to amplify and transmit comparable levels of the infectious agent. Here, the results are reported of a novel experiment designed specifically to assess the effects of serial passage of foot-and-mouth disease virus (FMDV) in experimental groups of sheep. A virus isolate taken from an epidemic of foot-and-mouth disease (FMD) characterized by rapid fade-out of infection was passed serially through four groups of sheep housed in an isolation unit. Although it was not possible to measure individual infectiousness directly, blood virus load from infected individuals was quantified using a real-time PCR assay and used as an underlying indicator of the level of infection. The results of this assay concurred well with those of the traditional tissue-culture assay and were shown to be highly repeatable. The level of peak viraemia was shown to fall significantly with the time of infection and with passage group, both in terms of the group mean and regression analysis of individual values, suggesting that this isolate of FMDV may, under certain conditions, be unable to maintain itself indefinitely in susceptible sheep populations. The results of these experiments are discussed in terms of the epidemiology of FMD in sheep.

Properties of foot-and-mouth disease virus in tissue culture

1960 ◽  
Vol 9 (5) ◽  
pp. 582-593 ◽  
Author(s):  
Z. Dinter ◽  
L. Philipson ◽  
T. Wessl�n
Keyword(s):  
Tissue Culture ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Replication and attenuation of foot and mouth disease virus in chick embryo

2012 ◽  
Vol 9 (1) ◽  
pp. 31-35
Author(s):  
Baghdad Science Journal
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Guinea Pig ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Challenge Virus ◽  
Mouth Disease Virus ◽  
Attenuated Virus ◽  
Foot And Mouth

This study includes replication and attenuation of foot and mouth disease virus type O which isolated from infected calves. Many passages for the virus in chick-Embryo were established as a substitute method to the tissue culture which is highly caustic in contrast to the chick embryo. The virus passed ten consequent passages which lead to the reduce of the titer of the virus from 106.53 TCID50/ 0.1 ml in cattle testis tissue culture to 103 TCID50/ 0.1 ml. the pathogenecity of attenuated FMD virus were also studied in both chick-embryo and guinea pigs. Using agar gel diffusion test precipitation antibodies was detected in guinea pig serum after 14 and 21 days post exposure to the attenuated virus. The inoculated guinea pig group with the chick-embryo attenuated virus appear resistance to the challenge virus. The result suggested the efficacy of attenuation of foot and mouth disease virus by using chick-embryo system for immunization against this disease on the level of laboratory Animal.

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