The effect of peptides containing the arginine-glycine-aspartic acid sequence on the adsorption of foot-and-mouth disease virus to tissue culture cells

Virus Genes ◽  
1990 ◽  
Vol 4 (1) ◽  
pp. 73-83 ◽  
Author(s):  
Barry Baxt ◽  
Yechiel Becker
Keyword(s):  
Tissue Culture ◽  
Aspartic Acid ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Arginine Glycine Aspartic Acid

Properties of foot-and-mouth disease virus in tissue culture

1960 ◽  
Vol 9 (5) ◽  
pp. 582-593 ◽  
Author(s):  
Z. Dinter ◽  
L. Philipson ◽  
T. Wessl�n
Keyword(s):  
Tissue Culture ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Replication and attenuation of foot and mouth disease virus in chick embryo

2012 ◽  
Vol 9 (1) ◽  
pp. 31-35
Author(s):  
Baghdad Science Journal
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Guinea Pig ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Challenge Virus ◽  
Mouth Disease Virus ◽  
Attenuated Virus ◽  
Foot And Mouth

This study includes replication and attenuation of foot and mouth disease virus type O which isolated from infected calves. Many passages for the virus in chick-Embryo were established as a substitute method to the tissue culture which is highly caustic in contrast to the chick embryo. The virus passed ten consequent passages which lead to the reduce of the titer of the virus from 106.53 TCID50/ 0.1 ml in cattle testis tissue culture to 103 TCID50/ 0.1 ml. the pathogenecity of attenuated FMD virus were also studied in both chick-embryo and guinea pigs. Using agar gel diffusion test precipitation antibodies was detected in guinea pig serum after 14 and 21 days post exposure to the attenuated virus. The inoculated guinea pig group with the chick-embryo attenuated virus appear resistance to the challenge virus. The result suggested the efficacy of attenuation of foot and mouth disease virus by using chick-embryo system for immunization against this disease on the level of laboratory Animal.

Crystalline Arrays of Foot-and-Mouth Disease Virus in Tissue Culture Cells

1967 ◽  
Vol 25 ◽  
pp. 102-103
Author(s):  
S.S. Breese ◽  
J.H. Graves
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Disease Virus ◽  
Kidney Tissue ◽  
Foot And Mouth Disease ◽  
Bovine Kidney ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Mouth Disease Virus ◽  
Swine Kidney

The formation of crystalline arrays of foot-and-mouth disease virus (FMDV) particles in swine kidney tissue culture cells has been examined in greater detail following the initial report of strain A119 in pig kidney cell line cultures, PK15. In the present experiments, FMDV strains recently isolated from field outbreaks in Argentina (A1, O2 and C3 CANEFA) were inoculated into primary swine kidney tissue cultures and samples at suitable intervals for embedding, sectioning and electron microscopy.The three strains of FMDV were stored at -20°C as the sixth bovine kidney tissue culture passage of original tongue tissue suspension. Immediately prior to use, they were inoculated into bovine kidney cultures and 1 ml of infected cell suspension was used in each swine kidney prescription bottle. After l/2 hr incubation at 37°C, 5 to 10 ml of maintenance medium was added and the bottles stored at 37°C. At intervals of 90 min, 3, 4, 5 and 6 hr after inoculation, bottles were processed for electron microscopy. Fluids were removed and the cells gently scraped from the glass into a few ml of Sorenson’s buffer at pH 7.2. Fixation was in 1% glutaraldehyde followed by 2% osmium tetroxide, dehydration, and embedment in epon. Sections were examined in an RCA-EMU-3-G microscope.

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