Thin-alginate-layer technique for protoplast culture of tobacco leaf protoplasts: Shoot formation in less than two weeks

PROTOPLASMA ◽  
1998 ◽  
Vol 204 (1-2) ◽  
pp. 114-118 ◽  
Author(s):  
A. Dovzhenko ◽  
U. Bergen ◽  
H. -U. Koop
Keyword(s):  
Protoplast Culture ◽  
Shoot Formation ◽  
Tobacco Leaf ◽  
Layer Technique ◽  
Leaf Protoplasts

scholarly journals The Effect of High pH and Calcium on Tobacco Leaf Protoplast Fusion

1973 ◽  
Vol 28 (11-12) ◽  
pp. 737-741 ◽  
Author(s):  
W. A. Keller ◽  
G Melchers
Keyword(s):  
Protoplast Fusion ◽  
Tobacco Leaf ◽  
High Frequencies ◽  
High Ph ◽  
Leaf Protoplast ◽  
Suitable Medium ◽  
Leaf Protoplasts

Abstract High frequencies of fusion were induced between isolated tobacco leaf protoplasts when they were incubated at 37 °C in the presence of 0.05 M CaCl2 in 0.4 ᴍ mannitol at pH 10.5. Subsequent to the fusion treatment the protoplasts were washed and cultured in a suitable medium and within two weeks, actively dividing cell colonies were observed. The fusion treatment had no irreversible, deleterious effects on the protoplasts

Organelles associated with the plasma membrane of tobacco leaf protoplasts

1983 ◽  
Vol 2 (6) ◽  
pp. 292-295 ◽  
Author(s):  
L. C. Fowke ◽  
P. J. Rennie ◽  
F. Constabel
Keyword(s):  
Plasma Membrane ◽  
Tobacco Leaf ◽  
Leaf Protoplasts

Endocytosis of polystyrene spheres by tobacco leaf protoplasts

1977 ◽  
Vol 105 (1) ◽  
pp. 127-135 ◽  
Author(s):  
M SUZUKI ◽  
I TAKEBE ◽  
S KAJITA ◽  
Y HONDA ◽  
C MATSUI
Keyword(s):  
Polystyrene Spheres ◽  
Tobacco Leaf ◽  
Leaf Protoplasts

A Quantitative Method to Monitor the Efficacy of Inhibitors Against the Chymotrypsin-Like Activity of the Proteasome in Tobacco Leaf Protoplasts

2014 ◽  
Vol 33 (4) ◽  
pp. 829-840
Author(s):  
Paolo Cascio ◽  
Fulvia Cerruti ◽  
Richard S. Marshall ◽  
Mary Raule ◽  
William Remelli ◽  
...  
Keyword(s):  
Quantitative Method ◽  
Tobacco Leaf ◽  
Leaf Protoplasts

Competence, determination, and meristemoid plasticity in tobacco organogenesis in vitro

2003 ◽  
Vol 81 (6) ◽  
pp. 611-621 ◽  
Author(s):  
Harbinder S Dhaliwal ◽  
Nicole S Ramesar-Fortner ◽  
Edward C Yeung ◽  
Trevor A Thorpe
Keyword(s):  
Root Formation ◽  
Leaf Explants ◽  
Basal Medium ◽  
Shoot Formation ◽  
Culture Conditions ◽  
Vascular Bundles ◽  
Mesophyll Cells ◽  
Tobacco Leaf ◽  
Organogenesis In Vitro

Tobacco leaf explants can produce both shoots and roots depending on the phytohormones in the medium. These arise directly via meristemoids (meristematic centers), which form distinct primordia and then organs. In this study it was found that shoot primordia arose from the palisade mesophyll cells at the adaxial surface, while root primordia arose from the rib parenchyma cells, near the existing vascular bundles. In studies on competency and determination, it was found that the tobacco leaf explants required 4–6 days in culture on a shoot-inducing medium (SIM) to become determined for shoot formation, while the explants were competent for rooting at excision and needed only 1 day on the root-inducing medium (RIM) to become determined for root formation. Transfer of explants from SIM or RIM to basal medium (BM without phytohormones) and vice versa supported the above findings. Transfer of explants from SIM to RIM and vice versa, delayed the timing of root and shoot formation, but not the position in the explant from which the organs arose. On transfer from SIM to RIM or vice versa, meristemoids that were already determined for shoot or root formation continued to develop, while those not yet determined were inhibited and (or) reverted to parenchymatous tissue. Thus under our culture conditions meristemoids in tobacco leaf explants are not plastic.Key words: competence, determination, meristemoid plasticity, organogenesis, tobacco.

Auxin-binding by particulate fractions from tobacco leaf protoplasts

Planta ◽  
1980 ◽  
Vol 150 (1) ◽  
pp. 9-12 ◽  
Author(s):  
D. Vreugdenhil ◽  
P. A. A. Harkes ◽  
K. R. Libbenga
Keyword(s):  
Tobacco Leaf ◽  
Auxin Binding ◽  
Leaf Protoplasts
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