Electroporation-mediated infection of tobacco leaf protoplasts with tobacco mosaic virus RNA and cucumber mosaic virus RNA

1986 ◽  
Vol 5 (1) ◽  
pp. 57-60 ◽  
Author(s):  
M. Nishiguchi ◽  
W. H. R. Langridge ◽  
A. A. Szalay ◽  
M. Zaitlin
Keyword(s):  
Tobacco Mosaic Virus ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Tobacco Leaf ◽  
Virus Rna ◽  
Leaf Protoplasts

Deletion analysis of the 5' untranslated leader sequence of tobacco mosaic virus RNA.

1991 ◽  
Vol 65 (3) ◽  
pp. 1619-1622 ◽  
Author(s):  
N Takamatsu ◽  
Y Watanabe ◽  
T Iwasaki ◽  
T Shiba ◽  
T Meshi ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Leader Sequence ◽  
Deletion Analysis ◽  
Virus Rna

Nucleotide sequence and translation of satellite tobacco mosaic virus RNA

Virology ◽  
1989 ◽  
Vol 170 (1) ◽  
pp. 139-146 ◽  
Author(s):  
T.E. Mirkov ◽  
D.M. Mathews ◽  
D.H. Du Plessis ◽  
J.A. Dodds
Keyword(s):  
Nucleotide Sequence ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Rna ◽  
Satellite Tobacco Mosaic Virus

Methods for cDNA cloning and sequencing tobacco mosaic virus RNA

Gene ◽  
1984 ◽  
Vol 29 (3) ◽  
pp. 331-342 ◽  
Author(s):  
Philip Goelet ◽  
Jonathan Karn
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Cdna Cloning ◽  
Cloning And Sequencing ◽  
Virus Rna

Molecular cloning of the complementary DNA copies of the common and cowpea strains of tobacco mosaic virus RNA

Virology ◽  
1982 ◽  
Vol 118 (1) ◽  
pp. 64-75 ◽  
Author(s):  
Tetsuo Meshi ◽  
Nobuhiko Takamatsu ◽  
Takeshi Ohno ◽  
Yoshimi Okada
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Molecular Cloning ◽  
Complementary Dna ◽  
Virus Rna ◽  
The Common

scholarly journals Evaluation of SDS-Polyacrylamide gel electrophoresis and immunostrip technique for detection of Tobacco mosaic virus and Cucumber mosaic virus in IRAQ

2011 ◽  
Vol 5 (2) ◽  
pp. 85-94
Author(s):  
Rakib A. Al-Ani ◽  
Mustafa A. Adhab
Keyword(s):  
Tobacco Mosaic Virus ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Positive Reaction ◽  
Total Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Protein Band ◽  
Polyacrylamide Gel ◽  
Total Proteins ◽  
Winter Squash

his study was carried out to evaluate the efficiency of electrophoresis on SDS- poly acrylamide slap gel and immunostrip techniques for detection of Tobacco mosaic virus (TMV, genus Tobamovirus) and Cucumber mosaic virus (CMV, genus Cucumovirus, family Bromoviridae), compared with symptoms on diagnostic plants for the two viruses. The results obtained showed that the two methods were effective. The analysis of samples of purified CMV, total proteins from infected cucumber plants, and extracts from infected plants with or without chlorophyll, by electrophoresis on 10% polyacrylamide slap gel containing 0.1% SDS showed two bands of 24 and 26 kd in size, and absent in samples of total protein or extracts of healthy plants. These two proteins represent the coat protein (CP) of CMV. In addition, one 18 kd protein band appeared on SDS- polyacrylamide gel profile which represent the CP of TMV, when samples of purified virus, total protein of infected plants, and plant extracts with or without chlorophyll were analyzed. This band was absent in similar samples from healthy plants. The test of immunostrip specific for CMV showed positive reaction with extracts from melon, cucumber, winter squash, and zucchini infected plants. Similarly, a positive reaction with immunostrip specific for TMV appeared with extracts from tobacco, tomato infected with TMV. No reaction was obtained with healthy plants extract. These results were similar to those obtained from indicator plants for the two viruses.

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