Lightmicroscopical histochemistry of hydrolases in the root tip of lima beans,Phaseolus lunatus L., in relation to cell differentiation and wound regeneration

PROTOPLASMA ◽  
1977 ◽  
Vol 91 (3) ◽  
pp. 229-242 ◽  
Author(s):  
Claire V. Thomas ◽  
J. R. Esterly ◽  
M. Ruddat
Keyword(s):  
Cell Differentiation ◽  
Phaseolus Lunatus ◽  
Root Tip ◽  
Lima Beans

scholarly journals First Report of Phytophthora capsici Infecting Lima Bean (Phaseolus lunatus) in the Mid-Atlantic Region

Plant Disease ◽  
2002 ◽  
Vol 86 (9) ◽  
pp. 1049-1049 ◽  
Author(s):  
C. R. Davidson ◽  
R. B. Carroll ◽  
T. A. Evans ◽  
R. P. Mulrooney ◽  
S. H. Kim
Keyword(s):  
Phytophthora Capsici ◽  
Petri Dish ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Pathogenicity Test ◽  
Atlantic Region ◽  
Kent County ◽  
Original Field ◽  
Lima Beans ◽  
Stem Lesions

Lima beans are an important crop in Delaware and the Mid-Atlantic Region. In the summer of 2000, five commercial cultivars (3–28, 184–85, C-elite Sel, Butter Bean, and Jackson Wonder) of lima bean in Delaware, Maryland, and New Jersey were observed with white, appressed mycelia on infected pods that appeared distinctly different from signs of downy mildew infection caused by Phytophthora phaseoli. Isolations were made by placing diseased pods between layers of rye media (1). A fungus that produced white mycelia with sporangia was consistently isolated. All Phytophthora isolates from the infected pods were heterothallic, grew at 35°C, had as much as 100 μm long pedicles on varying shapes of caducous sporangia with tapering base and >2 papillae, and were identified as P. capsici (2). Initially, three surface-disinfected pods from cv. Early Thorogreen plants grown in the greenhouse were floated on 20 ml of sterile water in a petri dish, and each was inoculated with a disk of P. capsici. This was repeated for nine isolates obtained from lima bean. After incubation for 7 days at room temperature, all 27 pods were infected, and P. capsici was reisolated from all the pods. A pathogenicity test was performed on the same cultivars from which the original field isolates were collected. Three seedlings and two plants with mature pods were inoculated with a sporangial suspension of each of the nine isolates and placed in a dew chamber for 5 days at 20 to 25°C and 100% relative humidity. White mycelial growth was observed on seedlings and mature pods. One inoculated plant developed brown-to-black stem lesions with white mycelia. All pods on the mature plants showed appressed, white mycelia identical to that observed in the commercial lima bean fields. P. capsici was consistently reisolated from all inoculated plants. In 2000, most infected pods in infested fields were observed low in the plant canopy or touching the soil. However, in 2001, infected pods were mostly in the lower and mid-portion of the plants observed in baby lima bean fields in Kent County, DE. References: (1) C. E. Caten and J. L. Jinks. Can. J. Bot. 46:329, 1967. (2) D. C. Erwin and O. K. Ribeiro. Phytophthora capsici. Page 264 in: Phytophthora Diseases Worldwide. The American Phytopathological Society, St Paul, MN, 1996.

scholarly journals Total phenolics, flavonoids, tannins and antioxidant activity of lima beans conserved in a Brazilian Genebank

2014 ◽  
Vol 45 (2) ◽  
pp. 335-341 ◽  
Author(s):  
Tânia da Silveira Agostini-Costa ◽  
Ana Flávia Pádua Teodoro ◽  
Rosa de Belem das Neves Alves ◽  
Leandro Ribeiro Braga ◽  
Ieler Ferreira Ribeiro ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Abiotic Factors ◽  
Condensed Tannin ◽  
Germplasm Collection ◽  
Lima Bean ◽  
Ex Situ ◽  
Phaseolus Lunatus ◽  
Beneficial Effects ◽  
Lima Beans

The objective of this study was to characterize for the first time polyphenols and DPPH (2-diphenyl-1-picryhydrazyl radical) antioxidant activity in commonly cultivated accessions of Phaseolus lunatus from an ex situ germplasm collection maintained by Embrapa, in Brazil. Furthermore, the study aimed to detect changes in total polyphenols, total flavonoids and condensed tannin for the same accessions after regeneration in a greenhouse. The results showed the diversity of the lima bean collection for phenolic compounds, which were strongly correlated with antioxidant activity. Lima beans accessions with the highest polyphenols and antioxidant activity were those with colored seeds. Conservation through cold storage of P. lunatus seeds in a cold chamber in the germplasm collection did not necessarily affect phenolic compounds. Variations observed in values after regeneration seeds may be mainly results of biotic and abiotic factors, including not only cultivar, but also environmental conditions. This study suggests that polyphenols in the lima beans present antioxidant activity, with possible beneficial effects for human health. It was expected that the potential of this tasty legume can be also used as a functional food crop and/or as a new ingredient in gastronomy.

Assessment of Wood Ash Application on Yield Advantage Indices of Maize and Lima Beans in an Intercrop

2019 ◽  
pp. 1-11 ◽  
Author(s):  
R. O. Ajala ◽  
M. A. Awodun ◽  
A. J. Adeyemo ◽  
B. F. Dada
Keyword(s):  
Zea Mays L ◽  
Block Design ◽  
Lima Bean ◽  
Wood Ash ◽  
Phaseolus Lunatus ◽  
Land Equivalent Ratio ◽  
University Of Technology ◽  
Randomized Complete Block Design ◽  
Lima Beans ◽  
Competition Indices

Alternate planting combinations of maize (Zea mays L.) with lima bean (Phaseolus lunatus L.) using wood ash as soil amendments were compared with the sole planting of each crop during the late 2014  and early 2015 planting seasons at the Teaching and Research Farm of the Federal University of Technology, Akure. The experiment in each season adopted three patterns of intercropping using ash as a soil amendment and laid out in a Randomized Complete Block Design (RCBD) with three replications. The experiment comprised 10 treatments: Sole planting of maize amended, sole planting of maize unamended, sole planting of lima beans amended and sole planting of lima beans unamended. Others were; 75:25 maize-lima beans amended, 75:25 maize-lima beans unamended, 50:50 maize-lima beans amended, 50:50 maize-lima beans unamended, 25:75 maize-lima beans amended and 25:75 maize-lima beans unamended. Wood ash was applied two weeks after planting at the rate of 2.4 kg (4 tons per hectare) to each plot. The combined yield advantage in terms of land equivalent ratio (LER) indices was greatest (1.95) in the case of 3 rows of maize and 1row of lima beans intercropping arrangements. Competition indices (CR) for all crops in all intercropping arrangements were more than 0.1 indicating that both crops were equally competitive.  However, crop aggressivity (A) showed that maize was more dominant than lima beans due to plant population. Costs and returns analysis revealed that maize and lima beans intercropping at all proportions were more profitable than their corresponding monocrops.

scholarly journals A Modular Cage System Design for Continuous Medium to Large ScaleIn VivoRearing of Predatory Mites (Acari: Phytoseiidae)

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Juan Alfredo Morales-Ramos ◽  
Maria Guadalupe Rojas
Keyword(s):  
Phytoseiulus Persimilis ◽  
Experimental Period ◽  
Predatory Mites ◽  
Interval Length ◽  
Phaseolus Lunatus ◽  
Modular System ◽  
Phytoseiid Mites ◽  
Cage System ◽  
Lima Beans

A new stackable modular system was developed for continuousin vivoproduction of phytoseiid mites. The system consists of cage units that are filled with lima beans,  Phaseolus lunatus, or red beans,P. vulgaris, leaves infested with high levels of the two-spotted spider mites,Tetranychus urticae. The cage units connect with each other through a connection cup, which also serves for monitoring and collection. Predatory mites migrate upwards to new cage units as prey is depleted. The system was evaluated for production ofPhytoseiulus persimilis. During a 6-month experimental period,20,894.9±10,482.5(mean ± standard deviation) predators were produced per week. The production consisted of4.1±4.6% nymphs and95.9±4.6% adults. A mean of554.5±59.8predatory mites were collected per harvested cage and the mean interval length between harvests was6.57±6.76days. The potential for commercial and experimental applications is discussed.

scholarly journals First Report of Mefenoxam-Resistant Isolates of Phytophthora capsici from Lima Bean Pods in the Mid-Atlantic Region

Plant Disease ◽  
2008 ◽  
Vol 92 (4) ◽  
pp. 656-656 ◽  
Author(s):  
J. F. Davey ◽  
N. F. Gregory ◽  
R. P. Mulrooney ◽  
T. A. Evans ◽  
R. B. Carroll
Keyword(s):  
Foliar Application ◽  
Phytophthora Capsici ◽  
Crop Protection ◽  
Lima Bean ◽  
Fruit Rot ◽  
Resistant Isolate ◽  
Phaseolus Lunatus ◽  
Atlantic Region ◽  
History Of ◽  
Lima Beans

Phytophthora capsici Leonian, the causal agent of lima bean pod rot, was first identified as a pathogen of lima bean in 2002 (1) and poses a new threat to lima bean (Phaseolus lunatus L.) production in the Mid-Atlantic Region. The phenylamide fungicide mefenoxam (Ridomil Gold; Syngenta Crop Protection) is widely used in the region for controlling foliar and soilborne diseases caused by Oomycetes. Isolates of P. capsici were collected from lima bean pods from production fields in Delaware, Maryland, and New Jersey from 1998 to 2004. These isolates originated from survey samples of lima bean fields for another pathogen, P. phaseoli, in 1999 and 2000 and diagnostic samples were submitted to the Plant Disease Clinic. Isolates were from lima bean, except for one from pepper (basal stem). Identification was made on the basis of morphometric characteristics. No known sensitive or insensitive isolates were included in the evaluation. Single zoospore cultures were evaluated for mefenoxam sensitivity on V8 agar plates amended with 100 ppm of mefenoxam, a previously tested concentration (2). Seven-millimeter-diameter agar plugs of each isolate were cut from the edge of actively expanding cultures of P. capsici with a cork borer and transferred to three V8 agar plates amended with mefenoxam and three unamended V8 plates. The plates were arranged in a completely randomized design and incubated at 25°C in the dark for 3 days. After incubation, colony growth was measured in millimeters and averaged for the three replicate plates of each isolate and percent growth relative to the unamended control was calculated. Mefenoxam sensitivity was assigned according to methods of Lamour et al. (2). The experiment was repeated once, and also run with a treatment of 200 ppm of mefenoxam. Of sixteen isolates screened, nine were rated as sensitive, four were intermediately resistant, and three were resistant. There was no difference between the 100 and 200 ppm results, except for a slight increase in sensitivity for one isolate. A subsequent experiment tested five isolates at concentrations of 1, 10, 100, and 1,000 ppm. Results were consistent with previous tests, with resistant isolates exhibiting some growth at the highest concentration of mefenoxam. One resistant isolate was from a field in Delaware previously cropped to slicing cucumbers with a history of mefenoxam applications. The second was from Caroline County, MD, which is heavily cropped to pickling cucumbers and likely to have been exposed to mefanoxam applications for the control of fruit rot; the origin of the third insensitive isolate from lima bean is unknown. Mefanoxam usage on lima bean is usually limited to one foliar application of mefenoxam+copper hydroxide to control downy mildew in the fall crop in wet seasons. This study indicates that mefenoxam resistance is present in populations of P. capsici in lima bean fields in the Mid-Atlantic Region, presumably as a result of mefenoxam applications to other vegetable crops, principally cucurbits, which are planted in rotation with lima beans or from nearby cucurbit fields. Implementing strategies to minimize fungicide resistance in other vegetables is important to slow resistance development associated with this emerging pathogen on lima beans. Lima bean pod rot continues to be seen sporadically each year in fields with a history of P. capsici and abundant rainfall or excessive irrigation. References: (1) C. R. Davidson et al. Plant Dis. 86:1049, 2002. (2) K. H. Lamour et al. Phytopathology 90:396, 2000.

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