Electron microscopic visualization of membrane Ca2+-binding sites in barley root tip cells: Inductionin vivo by seed germination in the presence of CaCl2

PROTOPLASMA ◽  
1983 ◽  
Vol 115 (2-3) ◽  
pp. 222-227 ◽  
Author(s):  
N. V. Belitser ◽  
G. V. Zaalishvili
PROTOPLASMA ◽  
1982 ◽  
Vol 111 (1) ◽  
pp. 63-78 ◽  
Author(s):  
N. V. Belitser ◽  
G. V. Zaalishvili ◽  
N. P. Sytnianskaja

2010 ◽  
Vol 13 (6) ◽  
pp. 2443-2449 ◽  
Author(s):  
Monica Ruffini Castiglione ◽  
Lucia Giorgetti ◽  
Chiara Geri ◽  
Roberto Cremonini

Caryologia ◽  
2021 ◽  
Author(s):  
Sazada Siddiqui ◽  
Saad Abdurahamn Muhammad Al Amri ◽  
Huda Ahmed Al Ghamdy ◽  
Wadha Saad Saeed Alqahtani ◽  
Sarah Mohammed Alquyr ◽  
...  

Bisphenol A (BPA) is a global transpiring pollutant and an endocrine disruptor present in the environment which has a substantial harmful effect on plants. In the present study, its effects on seed germination, radicle length and cytogenetic alterations were investigated in Pisum sativum L root tip cells. Pisum sativum L seeds were germinated after treating with various concentrations of BPA (2 mg/L, 5 mg/L, 10 mg/L, 15 mg/L, 20 mg/L and 25 mg/L) at 24±1°C for 72 hours and the cytogenetic variations were assessed. The investigation showed that BPA reduced the percentage of seed germination, mitotic index, radicle length (at higher concentrations) and instigated a rise in chromosomal anomalies in a dose-related manner. In total, there is an enhanced occurrence of c-mitosis, stickiness, bridges, fragments and laggards in the BPA treated root tip cells of Pisum sativum L seeds.


2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Skhawat Ali ◽  
Rong Jin ◽  
Rafaqat A. Gill ◽  
Theodore M. Mwamba ◽  
Na Zhang ◽  
...  

Beryllium (Be) could be a threatening heavy metal pollutant in the agroecosystem that may severely affect the performance of crops. The present study was conducted to evaluate the toxic effects of Be (0, 100, 200, and 400 μM) on physiological, ultrastructure, and biochemical attributes in hydroponically grown six-day-old seedlings of two cultivars ofBrassica napusL., one tolerant (ZS 758, black seeded) and one sensitive (Zheda 622, yellow seeded). Higher Be concentrations reduced the plant growth, biomass production, chlorophyll contents, and the total soluble protein contents. A significant accumulation of ROS (H2O2, OH−) and MDA contents was observed in a dose-dependent manner. Antioxidant enzymatic activities including SOD, POD, GR, APX, and GSH (except CAT) were enhanced with the increase in Be concentrations in both cultivars. Relative transcript gene expression of above-mentioned antioxidant enzymes further confirmed the alterations induced by Be as depicted from higher involvement in the least susceptible cultivar ZS 758 as compared to Zheda 622. The electron microscopic study showed that higher level of Be (400 μM) greatly damaged the leaf mesophyll and root tip cells. More damage was observed in cultivar Zheda 622 as compared to ZS 758. The damage in leaf mesophyll cells was highlighted as the disruption in cell wall, immature nucleus, damaged mitochondria, and chloroplast structures. In root tip cells, disruption in Golgi bodies and damage in cell wall were clearly noticed. As a whole, the present study confirmed that more inhibitory effects were recorded in yellow seeded Zheda 622 as compared to black seeded ZS 758 cultivar, which is regarded as more sensitive cultivar.


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