Phase contrast observations on the endoplasmic reticulum of living cells in culture

PROTOPLASMA ◽  
1965 ◽  
Vol 59 (3-4) ◽  
pp. 569-588 ◽  
Author(s):  
Ian K. Buckley
Keyword(s):  
Endoplasmic Reticulum ◽  
Phase Contrast ◽  
Living Cells ◽  
Cells In Culture

scholarly journals Observations on the Cytoplasmic Membranes of Testicular Cells, Examined by Phase Contrast and Electron Microscopy

1958 ◽  
Vol 4 (2) ◽  
pp. 135-142 ◽  
Author(s):  
Don W. Fawcett ◽  
Susumu Ito
Keyword(s):  
Endoplasmic Reticulum ◽  
Phase Contrast ◽  
Cell Types ◽  
Living Cells ◽  
Experimental Investigations ◽  
Isolated Cells ◽  
Testicular Cells ◽  
Linear Extent ◽  
Cytoplasmic Membranes ◽  
Myelin Figures

In freshly isolated cells of the guinea pig germinal epithelium examined with phase contrast, dark contours are seen in the cytoplasm that appear to be optical sections of the cisternae of the endoplasmic reticulum. These increase in contrast, in number, and in linear extent with increasing time up to 4 hours after isolation of the cells from the testis. During this period, cisternae originally present in the cells are extended and new ones appear to be formed by coalescence of tubular and vesicular elements of the reticulum. The cisternae become associated in parallel array and ultimately form elaborate concentric systems resembling structures that have often been interpreted as intracellular "myelin figures." Until now our knowledge of the endoplasmic reticulum has been based largely upon electron micrographs. The observation that the cisternae are visible in certain cell types under phase contrast optics opens the way for experimental investigations on the behavior of this class of cytoplasmic membranes in living cells.

Phase-contrast and electron-microscopic observations on a membranous complex in primary spermatocytes of the mouse

1975 ◽  
Vol 18 (1) ◽  
pp. 1-17
Author(s):  
A. Pleshkewych ◽  
L. Levine
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Phase Contrast ◽  
Cultured Cells ◽  
Light And Electron Microscopy ◽  
Cytoplasmic Inclusion ◽  
Electron Microscopic ◽  
Secondary Spermatocyte ◽  
Living Mouse ◽  
Circular Contour

A prominent cytoplasmic inclusion present in living mouse primary spermatocytes has been observed by both light and electron microscopy. It began to form at prometaphase and continued to increase in thickness and length as the cells developed. By metaphase it was a distinct sausage-shaped boundary that enclosed a portion of the cytoplasm between the spindle and the cell membrane. At the end of metaphase, the inclusion reached its maximum length. At telophase, it was divided between the daughter secondaries. The inclusion persisted as a circular contour in the interphase secondary spermatocyte. Electron microscopy of the same cultured cells that were previously observed with light microscopy revealed that the inclusion was a distinctive formation of membranes. It consisted of agranular cisternae and vesicles, and was therefore a membranous complex. Many of the smaller vesicles in the membranous complex resembled those found in the spindle. The cisternae in the membranous complex were identical to the cisternal endoplasmic reticulum of interphase primary spermatocytes. Nevertheless, the organization of vesicles and cisternae into the membranous complex was unique for the primaries in division stages, since such an organization was not present in their interphase stages.

scholarly journals Antibody-induced membrane fusion in Paramecium

1984 ◽  
Vol 65 (1) ◽  
pp. 153-162
Author(s):  
A. Barnett ◽  
E. Steers
Keyword(s):  
Electron Microscopy ◽  
Membrane Fusion ◽  
Phase Contrast ◽  
Immobilized Cells ◽  
Living Cells ◽  
Immune Serum ◽  
Induced Membrane ◽  
Transmission Electron ◽  
Membrane Changes ◽  
Antigen Antibody

Immobilization of cells by specific immune serum involves crosslinking between immunoglobulin G (IgG) and the i-antigen in the cell membrane. Globular material is seen to accumulate at the ciliary tips by phase-contrast and fluorescence microscopy in a manner analogous to ‘capping’ in more typical eukaryotes. When immobilized cells of Paramecium are examined by scanning electron microscopy, the fused ciliary tips are seen to be distended, discoidal membranes. Transmission electron microscopy often reveals several ciliary axonemes enclosed within a single, enlarged membrane that is oriented with the ferritin-labelled second antibody directed against the i-antigen antibody on the outer surface only. Fixed cells or living cells treated with immune Fab do not show membrane changes, but do bind antibody. Membrane fusion occurs only if cells are alive and the i-antigen is directly or indirectly cross-linked by intact immune IgG.

A tosylhydrazone-based probe for the ratiometric fluorescent detection of hypochlorite in endoplasmic reticulum of living cells

2022 ◽  
pp. 132382
Author(s):  
Xiao-Chuang Chang ◽  
Xue-Feng Han ◽  
Bing-Jie Liu ◽  
Zi-Yi Jiang ◽  
Shuai-Ze Li ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Living Cells ◽  
Fluorescent Detection

An endoplasmic reticulum-targeting and ratiometric fluorescent probe for hypochlorous acid in living cells based on a 1,8-naphthalimide derivative

2020 ◽  
Vol 44 (42) ◽  
pp. 18389-18398
Author(s):  
Qiujuan Ma ◽  
Chunyan Wang ◽  
Guojiang Mao ◽  
Meiju Tian ◽  
Jingguo Sun ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Endoplasmic Reticulum Targeting ◽  
Ratiometric Fluorescent Probe

A novel reticulum-targeting and ratiometric fluorescent probe for determining hypochlorous acid has been developed.

An Endoplasmic Reticulum-Targeted Ratiometric Fluorescent Probe for the Sensing of Hydrogen Sulfide in Living Cells and Zebrafish

2020 ◽  
Vol 92 (14) ◽  
pp. 9982-9988 ◽  
Author(s):  
Wei Shu ◽  
Shunping Zang ◽  
Chong Wang ◽  
Mengxu Gao ◽  
Jing Jing ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Ratiometric Fluorescent Probe

scholarly journals A Simple Probe for Super-Resolution Imaging of the Endoplasmic Reticulum in Living Cells

2018 ◽  
Vol 101 (11) ◽  
pp. e1800165 ◽  
Author(s):  
Elias A. Halabi ◽  
Salome Püntener ◽  
Pablo Rivera-Fuentes
Keyword(s):  
Endoplasmic Reticulum ◽  
Super Resolution ◽  
Living Cells ◽  
Resolution Imaging
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