Studien �ber den Infektionsverlauf bei Verimpfung des Tabakmosaik- und des Paratabakmosaikvirus auf Nicotiana glutinosa und Nicotiana Tabacum (var. White Burley)

1947 ◽  
Vol 3 (6) ◽  
pp. 303-326 ◽  
Author(s):  
Erich K�hler
Keyword(s):  
Nicotiana Tabacum ◽  
Nicotiana Glutinosa ◽  
White Burley

scholarly journals Evaluation the efficiency of different techniques for extraction and purification of Tomato yellow leaf curl virus (TYLCV)

2011 ◽  
Vol 8 (1) ◽  
pp. 447-452 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Incubation Period ◽  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Nicotiana Glutinosa ◽  
Citrate Buffer ◽  
Extraction And Purification ◽  
Standard Value ◽  
Leaf Curl Virus ◽  
White Burley ◽  
Leaf Curl

This study was conducted to evaluate the efficacy of different techniques for extraction and purification of Tomato yellow leaf curl virus (TYLCV). An isolate of the virus free of possible contamination with other viruses infecting the same host and transmitted by the same vector Bemisia tabaci Genn. was obtained. This was realized by indicator plants and incubation period in the vector. Results obtained revealed that the virus infect Nicotiana glutinosa without visible symptoms, while Nicotiana tabaccum var. White Burley was not susceptible to the virus. The incubation period of the virus in the vector was found to be 21 hrs. These results indicate that the virus is TYLCV. Results showed that Butanol was more effective in clarification the sap and eliminate of plant proteins and chlorophyll. The use of citrate buffer at pH 8 amended with reducing agents and EDTA to prevent the oxidation of phenolic compound was found to be suitable in maintaining the biological activity of the virus during extraction. The quantity of the virus obtained was 3.05 mg/100 gm leaves with absorption ratio of 1.4 at 260/280 nm which represent standard value for TYLCV.

scholarly journals Caracterização biológica de onze isolados de PVX (Potato virus X) do Brasil

2005 ◽  
Vol 29 (3) ◽  
pp. 521-527
Author(s):  
Oneida de Almeida Silva ◽  
Antonia dos Reis Figueira ◽  
Alessandra de Jesus Boari ◽  
César Augusto Brasil Pereira Pinto ◽  
Rafael Rodrigues Boni
Keyword(s):  
Nicotiana Tabacum ◽  
Potato Virus ◽  
Rio Grande ◽  
Potato Virus X ◽  
Rio Grande Do Sul ◽  
Nicotiana Glutinosa ◽  
Gomphrena Globosa ◽  
De Se ◽  
Distrito Federal ◽  
Das Elisa

Este trabalho foi realizado com onze isolados do Potato Vírus X (PVX), sendo seis do Centro de Indexação de Vírus Minas Gerais (VIC, LAV, UF, NR, SJ, UDI), um de São Paulo (VEL), dois do Rio Grande do Sul (P21 e P22), um de Santa Catarina (CAN) e um do Distrito Federal (BR). Os isolados de PVX foram inoculados mecanicamente, em duas épocas diferentes (setembro de 2001 e julho de 2002), em hospedeiras diversas, para verificação da sua agressividade e sintomatologia induzida nos hospedeiros, com a finalidade de se fazer sua diferenciação. A diagnose foi feita por inspeção visual e DAS-ELISA. Foram feitas também tentativas de recuperação dos vírus em plantas com DAS-ELISA negativo, por inoculação nas plantas indicadoras Gomphrena globosa e Nicotiana tabacum 'TNN'. Os sintomas induzidos pelos isolados de PVX na maioria das plantas foram semelhantes, com exceção de Nicotiana glutinosa e Physalis floridana, que se revelaram ótimas diferenciadoras para os isolados VEL e P21, respectivamente. Provavelmente os 11 isolados de PVX, estudados neste trabalho, possuem a mesma origem que os isolados dos grupos 1 e 3.

Tobacco Mosaic Virus-Infected Tissue

1985 ◽  
Vol 43 ◽  
pp. 510-511
Author(s):  
Egbert W. Henry
Keyword(s):  
Nicotiana Tabacum ◽  
Tobacco Mosaic Virus ◽  
Chlorogenic Acid ◽  
Mosaic Virus ◽  
Host Resistance ◽  
Oxidative Metabolism ◽  
Leaf Tissue ◽  
Vein Clearing ◽  
Basal Septum ◽  
Concentration Levels

Tobacco mosaic virus (TMV) infection has been studied in several investigations of Nicotiana tabacum leaf tissue. Earlier studies have suggested that TMV infection does not have precise infective selectivity vs. specific types of tissues. Also, such tissue conditions as vein banding, vein clearing, liquification and suberization may result from causes other than direct TMV infection. At the present time, it is thought that the plasmodesmata, ectodesmata and perhaps the plasmodesmata of the basal septum may represent the actual or more precise sites of TMV infection.TMV infection has been implicated in elevated levels of oxidative metabolism; also, TMV infection may have a major role in host resistance vs. concentration levels of phenolic-type enzymes. Therefore, enzymes such as polyphenol oxidase, peroxidase and phenylalamine ammonia-lyase may show an increase in activity in response to TMV infection. It has been reported that TMV infection may cause a decrease in o-dihydric phenols (chlorogenic acid) in some tissues.

Changes in polyamine synthesis during anther development and pollen germination in tobacco (Nicotiana tabacum)

1994 ◽  
Vol 92 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Fatiha Chibi ◽  
Angel Jesus Matilla ◽  
Trinidad Angosto ◽  
Dolores Garrido
Keyword(s):  
Nicotiana Tabacum ◽  
Pollen Germination ◽  
Anther Development ◽  
Polyamine Synthesis
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