Nucleotide sequence of an osmotin cDNA from the Nicotiana tabacum cv. White Burley generated by the polymerase chain reaction

1992 ◽  
Vol 18 (3) ◽  
pp. 621-622 ◽  
Author(s):  
Vinod Kumar ◽  
Margaret E. Spencer
Keyword(s):  
Polymerase Chain Reaction ◽  
Nicotiana Tabacum ◽  
Nucleotide Sequence ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
White Burley

scholarly journals Polymerase Chain Reaction Detection and Phylogenetic Characterization of an Agent Associated with Yellow Vine Disease of Cucurbits

1998 ◽  
Vol 88 (5) ◽  
pp. 428-436 ◽  
Author(s):  
Francisco J. Avila ◽  
Benny D. Bruton ◽  
Jacqueline Fletcher ◽  
J. L. Sherwood ◽  
Sam D. Pair ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleotide Sequence ◽  
16S Rdna ◽  
Fungal Pathogens ◽  
Pcr Amplification ◽  
Diagnostic Techniques ◽  
Chain Reaction ◽  
Phylogenetic Characterization ◽  
South Central ◽  
Polymerase Chain

Diagnosis of yellow vine disease (YVD) in cucurbits, an important disease in the south-central United States, relies on external symptom appearance, phloem discoloration, and the presence of bacterium-like organisms (BLOs) in phloem. Polymerase chain reaction (PCR) amplification of BLO nucleotide sequences was explored as a means to improve diagnostic techniques. PCR, using a primer pair based on sequences of the citrus-greening BLO, amplified a 0.15-kilobase (kb) fragment from the DNA of symptomatic plants, but not from that of asymptomatic plants. Its nucleotide sequence suggested that the DNA amplified was of pro-karyotic origin. A primer pair, designed to amplify nonspecific prokaryotic 16S rDNA, amplified a 1.5-kb DNA fragment in both the symptomatic and asymptomatic plants. The 1.5-kb fragment from the asymptomatic plants corresponded to chloroplast 16S rDNA, and the band from the symptomatic plants was composed of 16S rDNAs from both chloroplasts and a prokaryote. The nucleotide sequence of the prokaryotic DNA was determined and used to design three primers (YV1, YV2, and YV3). Fragments of 0.64 and 1.43 kb were amplified with primers YV1-YV2 and primers YV1-YV3, respectively, from symptomatic plants. Neither primer set yielded fragments from asymptomatic plants, unrelated bacteria, or selected soilborne fungal pathogens of cucurbits. Phylogenetic analysis indicated that the prokaryote is a gamma-3 proteobacterium. The consistent association of the 0.64- and 1.43-kb fragments with symptomatic plants suggests that the gamma-3 proteobacterium may be the causal agent of YVD of cantaloupe, squash, and watermelon.

scholarly journals Polymerase Chain Reaction Assays for the Detection and Discrimination of the Soybean Rust Pathogens Phakopsora pachyrhizi and P. meibomiae

2002 ◽  
Vol 92 (2) ◽  
pp. 217-227 ◽  
Author(s):  
Reid D. Frederick ◽  
Christine L. Snyder ◽  
Gary L. Peterson ◽  
Morris R. Bonde
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleotide Sequence ◽  
Real Time ◽  
Sequence Similarity ◽  
Morphological Characteristics ◽  
Its Region ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
Chain Reaction ◽  
Polymerase Chain

Soybean rust occurs in Australia and many countries throughout Africa, Asia, and South America. The causal agents of soybean rust are two closely related fungi, Phakopsora pachyrhizi and P. meibomiae, which are differentiated based upon morphological characteristics of the telia. Determination of the nucleotide sequence of the internal transcribed spacer (ITS) region revealed greater than 99% nucleotide sequence similarity among isolates of either P. pachyrhizi or P. meibomiae, but only 80% sequence similarity between the two species. Utilizing differences within the ITS region, four sets of polymerase chain reaction (PCR) primers were designed specifically for P. pachyrhizi and two sets for P. meibomiae. Classical and real-time fluorescent PCR assays were developed to identify and differentiate between P. pachyrhizi and P. meibomiae. Identification of P. pachyrhizi from infected soybean leaves using the real-time PCR assay will allow for more rapid diagnoses.

scholarly journals DNA sequence variation within maize and melon: observations from polymerase chain reaction amplification and direct sequencing.

Genetics ◽  
1990 ◽  
Vol 126 (1) ◽  
pp. 207-217 ◽  
Author(s):  
D M Shattuck-Eidens ◽  
R N Bell ◽  
S L Neuhausen ◽  
T Helentjaris
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleotide Sequence ◽  
Plant Species ◽  
Sequence Variation ◽  
Polymerase Chain Reaction Amplification ◽  
Chain Reaction ◽  
Base Substitutions ◽  
Polymerase Chain ◽  
Neutral Mutations ◽  
Genomic Regions

Abstract While compiling genetic linkage maps in several plant species based upon restriction fragment length polymorphisms (RFLPs), it was noted that the incidence of polymorphism differs among species. The basis of this disparity was investigated in this study by examining the nucleotide sequence at homologous loci among distinct cultivars within two species which exhibit considerably different levels of RFLPs. Using the polymerase chain reaction, homologous regions from different cultivars were first amplified and the nucleotide sequence of the products were determined. Four genomic regions of seven maize cultivars and three genomic regions of eight melon cultivars were examined to compare the respective levels of sequence variation between the two species. Levels of variation for both base substitutions and insertions/deletions varied widely among the maize sequences and between maize and melon for base substitutions. Estimates of theta (a measure of polymorphism) ranged from 0 to 0.002 in melon and from 0.006 to 0.040 for base substitutions and from 0.002 to 0.023 for insertions/deletions in maize. Critical value tests and chi-squared tests suggested that in maize the underlying processes generating and maintaining neutral mutations differ among the regions. The results not only suggest that several mechanisms are necessary to explain the variation seen in these two species, but also point to some basic dissimilarities in the organization and maintenance of the genomes of different plant species.

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