Fructose determination using immobilized enzymes in a flow system with special emphasis on the effect of isomerism

1988 ◽  
Vol 96 (1-6) ◽  
pp. 131-142 ◽  
Author(s):  
Mikael Skoog ◽  
Gillis Johansson ◽  
Bo Olsson ◽  
Roger Appelqvist
Keyword(s):  
Flow System ◽  
Immobilized Enzymes ◽  
Fructose Determination

Single- and coupled-enzyme nylon-tube reactors for plasma glucose determination with glucose oxidase and aldehyde dehydrogenase.

1980 ◽  
Vol 26 (12) ◽  
pp. 1652-1655 ◽  
Author(s):  
W Hinsch ◽  
A Antonijewić ◽  
P V Sundaram
Keyword(s):  
Glucose Oxidase ◽  
Aldehyde Dehydrogenase ◽  
Plasma Glucose ◽  
Continuous Flow ◽  
Flow System ◽  
Low Cost ◽  
Immobilized Enzymes ◽  
Glucose Determination ◽  
Nylon Tube

Abstract We describe routine methods for determining glucose in plasma with use of aldehyde dehydrogenase or glucose oxidase-aldehyde dehydrogenase immobilized in a nylon tube that is integrated into a continuous-flow system. Although the coupled-enzyme nylon-tube reactors require the presence of a third enzyme, catalase, in solution, the kinetics are not so complicated as to preclude reliable routine determination of glucose at very low cost. Precision is good, and results correlate well with those by the method involving glucose oxidase in solution. More than 3000 tests may be carried out with one reactor. The immobilized enzymes are stable for several months at 4 degrees C when not in use.

scholarly journals Amplified Detection of NAD+and NADH by the Enzymatic Cycling with the Use of Immobilized Enzymes in a Flow System

1992 ◽  
Vol 25 (9) ◽  
pp. 1631-1641 ◽  
Author(s):  
H. Mori ◽  
K. Yamashina ◽  
A. Asano ◽  
A. Uematsu
Keyword(s):  
Flow System ◽  
Immobilized Enzymes ◽  
Enzymatic Cycling

scholarly journals Determination of Glucose in Plasma Using Immobilized Enzymes in a Flow System.

2000 ◽  
Vol 46 (3) ◽  
pp. 219-222
Author(s):  
Hisakazu Mori ◽  
Miyuko Ando ◽  
Yoshie Sugi ◽  
Miyako Kimura ◽  
Katsuhiko Machida
Keyword(s):  
Flow System ◽  
Immobilized Enzymes

Modeling Trienzyme Biosensor at Internal Diffusion Limitation

2004 ◽  
Vol 9 (2) ◽  
pp. 139-144 ◽  
Author(s):  
J. Kulys
Keyword(s):  
Immobilized Enzymes ◽  
Internal Diffusion ◽  
Significant Response ◽  
Diffusion Limitation ◽  
Enzyme Reactions ◽  
Substrate Conversion ◽  
Apparent Stability ◽  
Biosensor Response

A model of biosensor containing three immobilized enzymes utilizing consecutive substrate conversion in the chain was developed. The modeling was performed at an internal diffusion limitation and a steadystate condition. The calculations showed that significant response of biosensors was produced if diffusion modules were larger than 1 for all enzyme reactions. Due to diffusion limitation the apparent stability of biosensor response increased many times in comparison to stability of the most labile enzyme of the chain.

Comparison of Optimal Pipe Sizing Designs for Pressurized Flow System

2018 ◽  
Vol 6 (8) ◽  
pp. 520-529
Author(s):  
Aanandsundar Arumugam ◽  
Gedion Habtay ◽  
Haben Kibrom ◽  
Medhanie Gebreamlak
Keyword(s):  
Flow System
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