scholarly journals Determination of Glucose in Plasma Using Immobilized Enzymes in a Flow System.

2000 ◽  
Vol 46 (3) ◽  
pp. 219-222
Author(s):  
Hisakazu Mori ◽  
Miyuko Ando ◽  
Yoshie Sugi ◽  
Miyako Kimura ◽  
Katsuhiko Machida
Keyword(s):  
Flow System ◽  
Immobilized Enzymes

Single- and coupled-enzyme nylon-tube reactors for plasma glucose determination with glucose oxidase and aldehyde dehydrogenase.

1980 ◽  
Vol 26 (12) ◽  
pp. 1652-1655 ◽  
Author(s):  
W Hinsch ◽  
A Antonijewić ◽  
P V Sundaram
Keyword(s):  
Glucose Oxidase ◽  
Aldehyde Dehydrogenase ◽  
Plasma Glucose ◽  
Continuous Flow ◽  
Flow System ◽  
Low Cost ◽  
Immobilized Enzymes ◽  
Glucose Determination ◽  
Nylon Tube

Abstract We describe routine methods for determining glucose in plasma with use of aldehyde dehydrogenase or glucose oxidase-aldehyde dehydrogenase immobilized in a nylon tube that is integrated into a continuous-flow system. Although the coupled-enzyme nylon-tube reactors require the presence of a third enzyme, catalase, in solution, the kinetics are not so complicated as to preclude reliable routine determination of glucose at very low cost. Precision is good, and results correlate well with those by the method involving glucose oxidase in solution. More than 3000 tests may be carried out with one reactor. The immobilized enzymes are stable for several months at 4 degrees C when not in use.

Determination of fast ozone oxidation rate for textile dyes by using a continuous quench-flow system

2010 ◽  
Vol 178 (1-3) ◽  
pp. 57-65 ◽  
Author(s):  
Arlindo C. Gomes ◽  
José C. Nunes ◽  
Rogério M.S. Simões
Keyword(s):  
Oxidation Rate ◽  
Flow System ◽  
Textile Dyes ◽  
Ozone Oxidation

scholarly journals Multi-pumping flow system for the determination of dissolved orthophosphate and dissolved organic phosphorus in wastewater samples

2006 ◽  
Vol 572 (1) ◽  
pp. 148-154 ◽  
Author(s):  
Carmen Pons ◽  
Ildikó V. Tóth ◽  
António O.S.S. Rangel ◽  
Rafael Forteza ◽  
Víctor Cerdà
Keyword(s):  
Flow System ◽  
Organic Phosphorus ◽  
Dissolved Organic Phosphorus ◽  
Wastewater Samples

Chemiluminescence flow system for the determination of Fe(II) and Fe(III) in water

1998 ◽  
Vol 360 (1) ◽  
pp. 130-132 ◽  
Author(s):  
W. Qin ◽  
Z. J. Zhang ◽  
F. C. Wang
Keyword(s):  
Flow System

Application of direct-injection detector integrated with the multi-pumping flow system to chemiluminescence determination of the total polyphenol index

2016 ◽  
Vol 911 ◽  
pp. 82-91 ◽  
Author(s):  
Edyta Nalewajko-Sieliwoniuk ◽  
Magdalena Iwanowicz ◽  
Sławomir Kalinowski ◽  
Anatol Kojło
Keyword(s):  
Flow System ◽  
Direct Injection ◽  
Total Polyphenol

Enzymes and Their Forms Used in Detection of Organophosphorus Compounds

2021 ◽  
pp. 22-41
Keyword(s):  
Organophosphorus Compounds ◽  
Degradation Products ◽  
Rapid Development ◽  
Hydrolytic Enzymes ◽  
Immobilized Enzymes ◽  
Amino Acid Sequences ◽  
Practical Implementation ◽  
Scientific Results ◽  
Physical And Chemical

Еnzymes are able to effectively interact with various organophosphorus compounds (OPC), entering into (bio)chemical reactions with them. Changes in the initial activity of enzymes as a result of their inhibition by OPC, the formation of OPC degradation products under the action of hydrolytic enzymes, etc. can be determined using different physical and chemical methods and used in bioanalytic systems to determine the concentrations of OPC. The purpose of the review is to analyze the main scientific results achieved over the past 10 years in the development of analytical systems based on enzymes intended for the determination of OPC. It is shown in the article, that the requirements for the sensitivity of biosensors are based on the norms of the content of the analyzed substances detected in/at the objects of mandatory control. The cholinesterases compose a basis for the development of the largest number of ultra-sensitive biosensors, although other enzymes can be successfully used as a biosensitive element. The most technologically advanced solution that is close to the practical implementation seems to be bioanalytical systems using immobilized enzymes. Improving the detection limits of the OPC can be achieved by using nanoobjects together with modern methods of signal transducers, for example, with nanomechanical detectors and signal converters. This combination of technical solutions ensures the sensitivity of the OPC analysis up to pg/l. At present, «reagentless» systems have received significant development, which have become the basis for the production of a large number of commercially available strips for the express determination of OPC. Modern demands stimulate the rapid development of portable and, especially, wearable biosensors that can be attached to various surfaces, including a clothing. The progress in the development of affine amino acid sequences, in the future, will allow the creation of enzyme biosensors on any surface.

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