Evaluation of diamine oxidase activity (DAO) in the rat intestinal mucosa by measuring expired14CO2 after oral administration of14C-putrescine

1994 ◽  
Vol 29 (1) ◽  
pp. 15-18 ◽  
Author(s):  
Tadao Bamba ◽  
Akira Sasaki ◽  
Shiro Hosoda
Keyword(s):  
Oral Administration ◽  
Intestinal Mucosa ◽  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Diamine Oxidase Activity

Release of intestinal diamine oxidase by histamine in rats

1983 ◽  
Vol 61 (4) ◽  
pp. 349-355 ◽  
Author(s):  
Armin Wollin ◽  
Henri Navert
Keyword(s):  
Intestinal Mucosa ◽  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Fold Increase ◽  
Lymph Flow ◽  
Intestinal Lumen ◽  
Enzyme Release ◽  
Storage Site ◽  
Diamine Oxidase Activity ◽  
Dose Dependent

Diamine oxidase activity was measured in the intestinal mucosa, lymph, and in the serum of rats, to determine whether histamine, a substrate of diamine oxidase, liberates this enzyme from its mucosal storage site(s). Histamine induced a sharp rise in intestinal lymph flow, lymph protein, and lymph diamine oxidase, lasting less than 1 h after the histamine injection. The rise in lymph diamine oxidase activity was dose dependent over a narrow concentration range (0.05–0.2 mmol/kg, i.v. and 0.15–0.6 mmol/kg i.d.). It did not correlate with the dose dependent increase in lymph flow or lymph protein. A single maximal intraduodenal dose of histamine caused a 41.6-fold increase in the lymph diamine oxidase activity and a 2.4-fold increase in the serum enzyme level temporarily. A second injection of histamine, 2 h after the first, resulted in a comparatively smaller increase in the lymph enzyme. The extent of the reduction was dependent on the magnitude of the first injection. The results suggest that histamine causes a limited liberation of diamine oxidase from the intestinal mucosa. The function of this enzyme release may be a protective response by the mucosa to reduce toxic levels of free histamine, either liberated by the mucosal tissue or absorbed from the intestinal lumen.

Nutrients regulate diamine oxidase release from intestinal mucosa

1998 ◽  
Vol 275 (4) ◽  
pp. R969-R975 ◽  
Author(s):  
Armin Wollin ◽  
Xiaolin Wang ◽  
Patrick Tso
Keyword(s):  
Intestinal Mucosa ◽  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Lipid Absorption ◽  
Intestinal Lumen ◽  
Enzyme Release ◽  
Distal Small Intestine ◽  
Intestinal Lymph ◽  
Diamine Oxidase Activity ◽  
Chylomicron Formation

Diamine oxidase is continuously released from the intestinal mucosa and carried to the circulation by the lymphatics. The effect of nutrients on this release was examined. Rats were prepared with duodenal and intestinal lymph cannulas. Test mixtures of lipid emulsions containing triolein, oleic acid, or tricaprylin and solutions of carbohydrate and protein were infused into the duodenum. The enzyme release and triglyceride transport were determined and in some experiments were done in the presence and absence of Pluronic L-81, an inhibitor of chylomicron formation, and aminoguanidine, an inhibitor of diamine oxidase activity. The data indicate that nonlipid nutrients did not increase diamine oxidase activity in the intestinal lymph, but the mucosal tissue content was significantly reduced in the distal small intestine, particularly after protein infusion. Triglycerides and fatty acids increased diamine oxidase in the intestinal lymph, and the longer-chain triglyceride was more effective. Inhibition of triglyceride transport did not interfere with the enzyme release, and the inhibition of diamine oxidase activity had no significant effect on lipid absorption. According to our observations, only lipids increase intestinal lymph diamine oxidase. Nonfat nutrients appear to increase diamine oxidase in the intestinal lumen. Diamine oxidase is not directly required for lipid absorption.

Histamine Metabolizing Activity of Dog Stomach and Intestine

1958 ◽  
Vol 195 (2) ◽  
pp. 525-527 ◽  
Author(s):  
Yutaka Kobayashi ◽  
A. C. Ivy
Keyword(s):  
Gastric Mucosa ◽  
Intestinal Mucosa ◽  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Control Tissue ◽  
Pyloric Mucosa ◽  
Diamine Oxidase Activity

The gastric mucosa of the dog was studied for histamine-metabolizing activity using only a microgram of C14-histamine. The intestinal mucosa which contains much diamine oxidase (histaminase) was used as a control tissue. No evidence of diamine oxidase was found in the cell-free extracts of the gastric mucosa though much diamine-oxidase activity was present in the cell-free extracts of the intestinal mucosa. A small amount of histamine destruction not inhibited by aminoguanidine and semicarbazide occurred in minced tissue digests of both the fundic and pyloric mucosa.

PP165-MON THE INFLUENCE OF MENSTRUAL CYCLE ON SERUM DIAMINE OXIDASE ACTIVITY IN HEALTHY WOMEN

2011 ◽  
Vol 6 (1) ◽  
pp. 176-177
Author(s):  
Y. Hamada ◽  
Y. Shinohara ◽  
M. Yano ◽  
M. Yamamoto ◽  
M. Yoshio ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Healthy Women ◽  
Diamine Oxidase Activity

Intestinal Permeability and Postheparin Plasma Diamine Oxidase Activity in the Prediction of Crohnʼs Disease Relapse

1999 ◽  
Vol 5 (2) ◽  
pp. 85-91 ◽  
Author(s):  
Robert J. Hilsden ◽  
Jon B. Meddings ◽  
James Hardin ◽  
Grant D. Gall ◽  
Lloyd R. Sutherland
Keyword(s):  
Intestinal Permeability ◽  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Disease Relapse ◽  
Diamine Oxidase Activity

Elevated Histaminase (Diamine Oxidase) Activity in Small-Cell Carcinoma of the Lung

1975 ◽  
Vol 293 (25) ◽  
pp. 1286-1290 ◽  
Author(s):  
Stephen B. Baylin ◽  
Martin D. Abeloff ◽  
Kathleen C. Wieman ◽  
J. Walton Tomford ◽  
David S. Ettinger
Keyword(s):  
Cell Carcinoma ◽  
Small Cell Carcinoma ◽  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Small Cell ◽  
Diamine Oxidase Activity

scholarly journals Relationship between postnatal days, serum Cu concentration and plasma diamine oxidase activity in Japanese Black calves

2020 ◽  
Vol 82 (10) ◽  
pp. 1488-1491
Author(s):  
Tatsuya FUKUDA ◽  
Kenji TSUKANO ◽  
Marina OTSUKA ◽  
Yoshiki MURAKAMI ◽  
Yasuyuki KITADE ◽  
...  
Keyword(s):  
Oxidase Activity ◽  
Diamine Oxidase ◽  
Diamine Oxidase Activity
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