Altered carbohydrate composition in colorectal adenomas and carcinomas: Histochemical characterization of N-acetylgalactosamine,l-fucose, and o-acetylated sialic acid

1996 ◽  
Vol 31 (1) ◽  
pp. 24-32 ◽  
Author(s):  
Kazuhide Iwakawa ◽  
Norifumi Ueda ◽  
Shinichi Murao ◽  
Nobuaki Kobayashi
Keyword(s):  
Sialic Acid ◽  
Colorectal Adenomas ◽  
Carbohydrate Composition ◽  
Histochemical Characterization

scholarly journals CHEMICAL CHARACTERIZATION OF GLYCOPEPTIDES FROM HUMAN γM-GLOBULINS

1968 ◽  
Vol 128 (4) ◽  
pp. 699-713 ◽  
Author(s):  
Joseph M. Davie ◽  
C. Kirk Osterland
Keyword(s):  
Molecular Weight ◽  
Sialic Acid ◽  
Gel Filtration ◽  
Chemical Characterization ◽  
Carbohydrate Composition ◽  
Total Percentage ◽  
Group I ◽  
Group Ii ◽  
Group 1

A survey of human pathological macroglobulins revealed that γM can be divided into at least two groups on the basis of carbohydrate composition. Differences between the two groups exist in the total percentage of carbohydrate (10.69 ± 1.49% for group 1, 7.71 ± 0.65% for group II) which is attributable to variation in hexose content. Glycopeptides from macroglobulins of each group were purified from pronase digests and characterized chemically. Macroglobulins from each group contain three types of oligosaccharides. Glycopeptide I for each group consisted of mannose, galactose, and NAG with a ratio of 3:2:1 for group I and a ratio of 2:1:2 for group II. Glycopeptide II consisted of mannose, galactose, and NAG (9:1:2) for group I, and mannose, fucose, galactose, and NAG (2:1:3:2) for group II. Glycopeptide III in both groups consisted of mannose, fucose, galactose, NAG, and sialic acid with a ratio of 6:2.5:2.5:5.5:2 for group I and a ratio of 5:1:1:6:1 for group II. Molecular weight estimations by gel filtration indicates that there are 10 glycopeptides I and II and 20 units of glycopeptide III per molecule of γM.

Histochemical characterization of the sialic acid residues in mouse colon mucins

2012 ◽  
Vol 76 (2) ◽  
pp. 156-162 ◽  
Author(s):  
Maria Mastrodonato ◽  
Donatella Mentino ◽  
Giuseppa Esterina Liquori ◽  
Domenico Ferri
Keyword(s):  
Sialic Acid ◽  
Mouse Colon ◽  
Histochemical Characterization

scholarly journals Purification and enzymatic characterization of CMP-sialic acid: beta-galactosyl1----3-N-acetylgalactosaminide alpha 2----3-sialyltransferase from human placenta.

1985 ◽  
Vol 260 (8) ◽  
pp. 4941-4951
Author(s):  
D H Joziasse ◽  
M L Bergh ◽  
H G ter Hart ◽  
P L Koppen ◽  
G J Hooghwinkel ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Human Placenta ◽  
Enzymatic Characterization

Glycoprotein coat of the TA3 cell. Isolation and partial characterization of a sialic acid containing glycoprotein fraction

Biochemistry ◽  
1972 ◽  
Vol 11 (14) ◽  
pp. 2559-2564 ◽  
Author(s):  
John F. Codington ◽  
Barbara H. Sanford ◽  
Roger W. Jeanloz
Keyword(s):  
Sialic Acid ◽  
Cell Isolation ◽  
Partial Characterization

scholarly journals Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium

2015 ◽  
Vol 87 (2) ◽  
pp. 997-1005 ◽  
Author(s):  
Jinqiang Hu ◽  
Tao Wei ◽  
Siwen Sun ◽  
Aijing Zhao ◽  
Chunping Xu
Keyword(s):  
Cigarette Smoke ◽  
Carbohydrate Composition ◽  
Cigarette Smoke Condensate ◽  
Essential Factor ◽  
Standard Strain ◽  
Acetic Acid Production ◽  
Bifidobacterium Animalis ◽  
Molecular Determination

The aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium. Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC). The standard strain of Bifidobacterium animalis was cultured in MRS media under anaerobic addition of CSC. The results showed that CSC significantly decreased the growth of B. animalis as well as EPSs and acetic acid production. Furthermore, two EPSs fractions (Fr-I and Fr-II) were isolated and purified for chemical and molecular determination. By comparison with control, CSC was found to be of great impact on EPSs carbohydrate composition. The molecular weight mass of Fr-I changed from 3.33×105 g/mol (without CSC) to 2.99×105 (with CSC). In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.

Histochemical characterization of the surface mucoprotein of normal and abnormal human trophoblast

1970 ◽  
Vol 2 (4) ◽  
pp. 263-274 ◽  
Author(s):  
S. Bradbury ◽  
W. D. Billington ◽  
D. R. S. Kirby ◽  
E. A. Williams
Keyword(s):  
Human Trophoblast ◽  
Histochemical Characterization
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