Histochemical characterization of the sialic acid residues in mouse colon mucins

2012 ◽  
Vol 76 (2) ◽  
pp. 156-162 ◽  
Author(s):  
Maria Mastrodonato ◽  
Donatella Mentino ◽  
Giuseppa Esterina Liquori ◽  
Domenico Ferri
Keyword(s):  
Sialic Acid ◽  
Mouse Colon ◽  
Histochemical Characterization

scholarly journals Purification and enzymatic characterization of CMP-sialic acid: beta-galactosyl1----3-N-acetylgalactosaminide alpha 2----3-sialyltransferase from human placenta.

1985 ◽  
Vol 260 (8) ◽  
pp. 4941-4951
Author(s):  
D H Joziasse ◽  
M L Bergh ◽  
H G ter Hart ◽  
P L Koppen ◽  
G J Hooghwinkel ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Human Placenta ◽  
Enzymatic Characterization

Glycoprotein coat of the TA3 cell. Isolation and partial characterization of a sialic acid containing glycoprotein fraction

Biochemistry ◽  
1972 ◽  
Vol 11 (14) ◽  
pp. 2559-2564 ◽  
Author(s):  
John F. Codington ◽  
Barbara H. Sanford ◽  
Roger W. Jeanloz
Keyword(s):  
Sialic Acid ◽  
Cell Isolation ◽  
Partial Characterization

Histochemical characterization of the surface mucoprotein of normal and abnormal human trophoblast

1970 ◽  
Vol 2 (4) ◽  
pp. 263-274 ◽  
Author(s):  
S. Bradbury ◽  
W. D. Billington ◽  
D. R. S. Kirby ◽  
E. A. Williams
Keyword(s):  
Human Trophoblast ◽  
Histochemical Characterization

scholarly journals Exo-enzymatic addition of diazirine-modified sialic acid to cell surfaces enables photocrosslinking of glycoproteins

2021 ◽  
Author(s):  
Nageswari Yarravarapu ◽  
Rohit Sai Reddy Konada ◽  
Narek Darabedian ◽  
Nichole J. Pedowtiz ◽  
Soumya N. Krishnamurthy ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Cell Types ◽  
Cell Surfaces ◽  
Binding Interactions ◽  
Multiple Cell ◽  
Labeling Method ◽  
Cell Surface Glycoconjugates

Glycan binding often mediates extracellular macromolecular recognition events. Accurate characterization of these binding interactions can be difficult because of dissociation and scrambling that occur during purification and analysis steps. Use of photocrosslinking methods has been pursued to covalently capture glycan-dependent interactions in situ however use of metabolic glycan engineering methods to incorporate photocrosslinking sugar analogs is limited to certain cell types. Here we report an exo-enzymatic labeling method to add a diazirine-modified sialic acid (SiaDAz) to cell surface glycoconjugates. The method involves chemoenzymatic synthesis of diazirine-modified CMP-sialic acid (CMP-SiaDAz), followed by sialyltransferase-catalyzed addition of SiaDAz to desialylated cell surfaces. Cell surface SiaDAz-ylation is compatible with multiple cell types and is facilitated by endogenous extracellular sialyltransferase activity present in Daudi B cells. This method for extracellular addition of α2-6-linked SiaDAz enables UV-induced crosslinking of CD22, demonstrating the utility for covalent capture of glycan-mediated binding interactions.

scholarly journals Histological and Histochemical Characterization of the Midgut of Healthy Aedes aegypti Larvae

2018 ◽  
Vol 22 (1) ◽  
pp. 1-15 ◽  
Author(s):  
A Lemos ◽  
F Adam ◽  
K Moura ◽  
L Moraes ◽  
O Silva
Keyword(s):  
Aedes Aegypti ◽  
Histochemical Characterization

Characterization of the Binding Affinity of Siglec-1 to gp120, gp145, and V2 Loop via Sialic Acid Binding Motif

2014 ◽  
Vol 30 (S1) ◽  
pp. A119-A120
Author(s):  
Hung V. Trinh ◽  
Ousman Jobe ◽  
Guofen Gao ◽  
Carl R. Alving ◽  
Venigalla Rao ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Binding Affinity ◽  
Binding Motif ◽  
Sialic Acid Binding
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