Immunocytochemical evidence for the localization of the GM1 ganglioside in carbonic anhydrase-containing and RT 97-immunoreactive rat primary sensory neurons

1989 ◽  
Vol 18 (1) ◽  
pp. 77-86 ◽  
Author(s):  
Brita Robertson ◽  
Gunnar Grant
Keyword(s):  
Carbonic Anhydrase ◽  
Sensory Neurons ◽  
Gm1 Ganglioside ◽  
Primary Sensory Neurons

Carbonic anhydrase activity in primary sensory neurons

1986 ◽  
Vol 245 (3) ◽  
Author(s):  
Ibtissam Barakat ◽  
Jerzy Kazimierczak ◽  
Bernard Droz
Keyword(s):  
Carbonic Anhydrase ◽  
Sensory Neurons ◽  
Carbonic Anhydrase Activity ◽  
Primary Sensory Neurons

Carbonic anhydrase activity in primary sensory neurons

1986 ◽  
Vol 245 (3) ◽  
Author(s):  
Jerzy Kazimierczak ◽  
ErnstW. Sommer ◽  
Eric Philippe ◽  
Bernard Droz
Keyword(s):  
Carbonic Anhydrase ◽  
Sensory Neurons ◽  
Carbonic Anhydrase Activity ◽  
Primary Sensory Neurons

scholarly journals Analyzing the Carotenoid Composition of Melilot (Melilotus officinalis (L.) Pall.) Extracts and the Effects of Isolated (All-E)-lutein-5,6-epoxide on Primary Sensory Neurons and Macrophages

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 503
Author(s):  
Györgyi Horváth ◽  
Eszter Csikós ◽  
Eichertné Violetta Andres ◽  
Tímea Bencsik ◽  
Anikó Takátsy ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Sensory Neurons ◽  
Biological Activities ◽  
Water Soluble ◽  
Soluble Form ◽  
Primary Sensory Neurons ◽  
Isolation And Identification ◽  
Melilotus Officinalis ◽  
Carotenoid Composition ◽  
Anti Inflammatory

Melilotus officinalis is known to contain several types of secondary metabolites. In contrast, the carotenoid composition of this medicinal plant has not been investigated, although it may also contribute to the biological activities of the drug, such as anti-inflammatory effects. Therefore, this study focuses on the isolation and identification of carotenoids from Meliloti herba and on the effect of isolated (all-E)-lutein 5,6-epoxide on primary sensory neurons and macrophages involved in nociception, as well as neurogenic and non-neurogenic inflammatory processes. The composition of the plant extracts was analyzed by high performance liquid chromatography (HPLC). The main carotenoid was isolated by column liquid chromatography (CLC) and identified by MS and NMR. The effect of water-soluble lutein 5,6-epoxide-RAMEB (randomly methylated-β-cyclodextrin) was investigated on Ca2+-influx in rat primary sensory neurons induced by the activation of the transient receptor potential ankyrin 1 receptor agonist to mustard-oil and on endotoxin-induced IL-1β release from isolated mouse peritoneal macrophages. (all-E)-Lutein 5,6-epoxide significantly decreased the percent of responsive primary sensory neurons compared to the vehicle-treated stimulated control. Furthermore, endotoxin-evoked IL-1β release from macrophages was significantly decreased by 100 µM lutein 5,6-epoxide compared to the vehicle-treated control. The water-soluble form of lutein 5,6-epoxide-RAMEB decreases the activation of primary sensory neurons and macrophages, which opens perspectives for its analgesic and anti-inflammatory applications.

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