Differential effects of distal and proximal nerve lesions on carbonic anhydrase activity in rat primary sensory neurons, ventral and dorsal root axons

1988 ◽  
Vol 70 (3) ◽  
Author(s):  
J.M. Peyronnard ◽  
L.F. Charron ◽  
J.P. Messier ◽  
J. Lavoie
Keyword(s):  
Carbonic Anhydrase ◽  
Sensory Neurons ◽  
Dorsal Root ◽  
Carbonic Anhydrase Activity ◽  
Primary Sensory Neurons ◽  
Nerve Lesions ◽  
Differential Effects

Carbonic anhydrase activity in primary sensory neurons

1986 ◽  
Vol 245 (3) ◽  
Author(s):  
Ibtissam Barakat ◽  
Jerzy Kazimierczak ◽  
Bernard Droz
Keyword(s):  
Carbonic Anhydrase ◽  
Sensory Neurons ◽  
Carbonic Anhydrase Activity ◽  
Primary Sensory Neurons

Carbonic anhydrase activity in primary sensory neurons

1986 ◽  
Vol 245 (3) ◽  
Author(s):  
Jerzy Kazimierczak ◽  
ErnstW. Sommer ◽  
Eric Philippe ◽  
Bernard Droz
Keyword(s):  
Carbonic Anhydrase ◽  
Sensory Neurons ◽  
Carbonic Anhydrase Activity ◽  
Primary Sensory Neurons

scholarly journals Enhanced T-type calcium channel 3.2 activity in sensory neurons contributes to neuropathic-like pain of monosodium iodoacetate-induced knee osteoarthritis

2020 ◽  
Vol 16 ◽  
pp. 174480692096380
Author(s):  
Seung Min Shin ◽  
Yongsong Cai ◽  
Brandon Itson-Zoske ◽  
Chensheng Qiu ◽  
Xu Hao ◽  
...  
Keyword(s):  
Sensory Neuron ◽  
Sensory Neurons ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Spinal Dorsal Horn ◽  
Primary Sensory Neurons ◽  
Primary Sensory Neuron ◽  
Spinal Dorsal ◽  
Osteoarthritis Pain ◽  
Monosodium Iodoacetate

The monosodium iodoacetate knee osteoarthritis model has been widely used for the evaluation of osteoarthritis pain, but the pathogenesis of associated chronic pain is not fully understood. The T-type calcium channel 3.2 (CaV3.2) is abundantly expressed in the primary sensory neurons, in which it regulates neuronal excitability at both the somata and peripheral terminals and facilitates spontaneous neurotransmitter release at the spinal terminals. In this study, we investigated the involvement of primary sensory neuron-CaV3.2 activation in monosodium iodoacetate osteoarthritis pain. Knee joint osteoarthritis pain was induced by intra-articular injection of monosodium iodoacetate (2 mg) in rats, and sensory behavior was evaluated for 35 days. At that time, knee joint structural histology, primary sensory neuron injury, and inflammatory gliosis in lumbar dorsal root ganglia, and spinal dorsal horn were examined. Primary sensory neuron-T-type calcium channel current by patch-clamp recording and CaV3.2 expression by immunohistochemistry and immunoblots were determined. In a subset of animals, pain relief by CaV3.2 inhibition after delivery of CaV3.2 inhibitor TTA-P2 into sciatic nerve was investigated. Knee injection of monosodium iodoacetate resulted in osteoarthritis histopathology, weight-bearing asymmetry, sensory hypersensitivity of the ipsilateral hindpaw, and inflammatory gliosis in the ipsilateral dorsal root ganglia, sciatic nerve, and spinal dorsal horn. Neuronal injury marker ATF-3 was extensively upregulated in primary sensory neurons, suggesting that neuronal damage was beyond merely knee-innervating primary sensory neurons. T-type current in dissociated primary sensory neurons from lumbar dorsal root ganglia of monosodium iodoacetate rats was significantly increased, and CaV3.2 protein levels in the dorsal root ganglia and spinal dorsal horn ipsilateral to monosodium iodoacetate by immunoblots were significantly increased, compared to controls. Perineural application of TTA-P2 into the ipsilateral sciatic nerve alleviated mechanical hypersensitivity and weight-bearing asymmetry in monosodium iodoacetate osteoarthritis rats. Overall, our findings demonstrate an elevated CaV3.2 expression and enhanced function of primary sensory neuron-T channels in the monosodium iodoacetate osteoarthritis pain. Further study is needed to delineate the importance of dysfunctional primary sensory neuron-CaV3.2 in osteoarthritis pain.

scholarly journals Somatostatin-like immunoreactive primary sensory neurons supplying the porcine adrenal glands in physiological conditions and after adrenalectomy

2014 ◽  
Vol 58 (4) ◽  
pp. 651-656 ◽  
Author(s):  
Sławomir Gonkowski
Keyword(s):  
Sensory Neurons ◽  
Dorsal Root ◽  
Adrenal Glands ◽  
Primary Sensory Neurons ◽  
Immunofluorescence Technique ◽  
Neuronal Tracing ◽  
Sensory Neurones ◽  
Physiological Conditions ◽  
Labelling Immunofluorescence

Abstract Retrograde neuronal tracing, using fast blue, in combination with a single-labelling immunofluorescence technique, was applied to determine whether somatostatin (SOM) participates in sensory innervating of the porcine adrenal glands in physiological conditions and after adrenalectomy. In control animals, SOM-like immunoreactive neurons comprised 7.0 ± 0.7% of adrenal gland-projecting cells in dorsal root ganglia (DRG) at neuromeres Th6-7 and 6.5 ± 1.2% at neuromeres Th12-14. After adrenalectomy the percentage of SOM-positive DRG cells considerably increased and attained the level of 44.7 ± 2.5% at neuromeres Th6-7 and 36.6 ± 1.7% at neuromeres Th12-14. The obtained results demonstrate that SOM is not only a neuromediator within sensory neurones supplying the porcine adrenal glands, but also suggest the role of this substance during repairing processes within the nervous system after adrenalectomy.

scholarly journals S100 protein-immunoreactive primary sensory neurons in the trigeminal and dorsal root ganglia of the rat

1997 ◽  
Vol 748 (1-2) ◽  
pp. 253-257 ◽  
Author(s):  
Hiroyuki Ichikawa ◽  
David M Jacobowitz ◽  
Tomosada Sugimoto
Keyword(s):  
Sensory Neurons ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
S100 Protein ◽  
Primary Sensory Neurons

scholarly journals AXL signaling in primary sensory neurons contributes to chronic compression of dorsal root ganglion-induced neuropathic pain in rats

2020 ◽  
Vol 16 ◽  
pp. 174480691990081 ◽  
Author(s):  
Lingli Liang ◽  
Jun Zhang ◽  
Lixia Tian ◽  
Shuo Wang ◽  
Linping Xu ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Root Ganglion ◽  
Sensory Neurons ◽  
Dorsal Root ◽  
Primary Sensory Neurons

scholarly journals Carbonic anhydrase activity in first-order sensory neurons of the rat.

1983 ◽  
Vol 31 (2) ◽  
pp. 293-300 ◽  
Author(s):  
V Wong ◽  
C P Barrett ◽  
E J Donati ◽  
L F Eng ◽  
L Guth
Keyword(s):  
Carbonic Anhydrase ◽  
Sensory Neurons ◽  
Carbonic Anhydrase Activity ◽  
First Order
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