Transfer of cytoplasm from newBeta CMS sources to sugar beet by asymmetric fusion

1990 ◽  
Vol 79 (3) ◽  
pp. 390-396 ◽  
Author(s):  
F. A. Krens ◽  
D. Jamar ◽  
G. J. A. Rouwendal ◽  
R. D. Hall
Keyword(s):  
2014 ◽  
Vol 64 (4) ◽  
pp. 341-347 ◽  
Author(s):  
Elżbieta Jażdżewska ◽  
Aleksandra Niklas ◽  
Anna Majewska-Sawka

The isolation and culture of suspension-derived protoplasts from two sugar beet (<em>Beta vulgaris</em> L.) genotypes are described. Immobilization of protoplasts in agarose resulted in high frequency divisions and microcallus regeneration, with plating efficiency (PE) being clearly genotype-dependent. In further studies towards asymmetric fusion experiments, the effect of different doses of ultraviolet radiation (UV) and iodoacetic acid (IA) on protoplast physiology was assessed. Viability of both treated (UV, IA) and untreated protoplasts (control) was determined by FDA staining, and the biological effect was evaluated by testing the ability of protoplasts to divide and to form calli. The results are discussed in terms of the applicability of the methods for the production of asymmetric protoplasts suitable for somatic hybridization within the genus <em>Beta</em>.


2001 ◽  
Vol 36 (1) ◽  
pp. 35-46 ◽  
Author(s):  
Khalid Fares ◽  
C. M. G. C. Renard ◽  
Qamar R'Zina ◽  
Jean-Francois Thibault
Keyword(s):  

1998 ◽  
Vol 23 (4) ◽  
pp. 347-353
Author(s):  
N. B. Kift ◽  
F. A. Mellon ◽  
A. M. Dewar ◽  
A. F. G. Dixon
Keyword(s):  

1991 ◽  
Vol 83 (1) ◽  
pp. 7-16 ◽  
Author(s):  
Christina Lind ◽  
Christer Hallden ◽  
Ian M. Moller
Keyword(s):  

1995 ◽  
Vol 94 (2) ◽  
pp. 342-350 ◽  
Author(s):  
Steffen Lenzner ◽  
Kurt Zoglauer ◽  
Otto Schieder

Author(s):  
S.V. Meshcheryakov ◽  
◽  
I.S. Eremin ◽  
D.O. Sidorenko ◽  
M.S. Kotelev ◽  
...  
Keyword(s):  

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