scholarly journals Progress towards sugar beet improvement through somatic hybridization I. Inactivation of nuclei and cytoplasm in donor and recipient protoplasts

2014 ◽  
Vol 64 (4) ◽  
pp. 341-347 ◽  
Author(s):  
Elżbieta Jażdżewska ◽  
Aleksandra Niklas ◽  
Anna Majewska-Sawka
Keyword(s):  
Sugar Beet ◽  
Ultraviolet Radiation ◽  
Beta Vulgaris ◽  
High Frequency ◽  
Biological Effect ◽  
Somatic Hybridization ◽  
Iodoacetic Acid ◽  
Plating Efficiency ◽  
Asymmetric Fusion ◽  
Different Doses

The isolation and culture of suspension-derived protoplasts from two sugar beet (<em>Beta vulgaris</em> L.) genotypes are described. Immobilization of protoplasts in agarose resulted in high frequency divisions and microcallus regeneration, with plating efficiency (PE) being clearly genotype-dependent. In further studies towards asymmetric fusion experiments, the effect of different doses of ultraviolet radiation (UV) and iodoacetic acid (IA) on protoplast physiology was assessed. Viability of both treated (UV, IA) and untreated protoplasts (control) was determined by FDA staining, and the biological effect was evaluated by testing the ability of protoplasts to divide and to form calli. The results are discussed in terms of the applicability of the methods for the production of asymmetric protoplasts suitable for somatic hybridization within the genus <em>Beta</em>.

scholarly journals Electrofusion of mesophyll protoplasts from two varieties of sugar beet, (Beta vulgaris L.)

2020 ◽  
Vol 1 (1) ◽  
pp. 22-25
Author(s):  
Qutaiba Al-Nema ◽  
Mozahim AL-Mallah
Keyword(s):  
Cell Wall ◽  
Sugar Beet ◽  
Beta Vulgaris ◽  
Experimental Approach ◽  
Somatic Hybridization ◽  
Hybrid Cells ◽  
Mesophyll Protoplasts ◽  
Leaf Mesophyll ◽  
Msec Pulse ◽  
Protoplast Viability

Somatic hybridization between different plants through protoplast fusion represent an efficient experimental approach to produce genetically transformed plant species. Electrofution of mesophyll protoplasts in sugar beet was occurred to overcome the barriers faced breeding program of this economically industrial crop Protoplasts were successfully isolated from leave's mesophyll of two varieties of sugar beet (Beta vulgaris L.). Various enzyme solutions were assessed for the cell wall degrading ability. They express different efficiency in isolation of mesophyll protoplasts of var. Baraka. The protoplasts yield was 18 × 104 cell ml-1 using the mixture consisting of 0.5% Cellulase RS, 1.0% Hemicellulase and 0.1% Pectolyase Y-23 with 13% mannitol. A total of 16 hrs. for cell wall digestion, and protoplast viability approached 93%. Protoplasts were isolated from leaf mesophyll of var. Carola using the same enzymatic mixtures. High protoplasts yield 20 × 104 cell ml-1 was obtained, requiring the same period 16 hrs. to approach viability 96%. The protoplasts were spherical in shape, varied in chloroplast distribution, having size ranged 12 – 52 µm. The present study succeeded in electrofusion between Baraka × Carola mesophyll protoplasts, producing somatic hybrid cells under conditions of 1MHz, 1000 Vcm-1, 2 pulses, 1.5 msec./pulse with fusion percent of 73%.  

Plant regeneration from protoplasts of sugar beet (Beta vulgaris)

1995 ◽  
Vol 94 (2) ◽  
pp. 342-350 ◽  
Author(s):  
Steffen Lenzner ◽  
Kurt Zoglauer ◽  
Otto Schieder
Keyword(s):  
Plant Regeneration ◽  
Sugar Beet ◽  
Beta Vulgaris
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