Hair cell replacement in the avian inner ear following two exposures to intense sound

1995 ◽  
Vol 24 (2) ◽  
pp. 111-116 ◽  
Author(s):  
Henry J. Adler ◽  
James C. Saunders
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Cell Replacement ◽  
Avian Inner Ear

scholarly journals Identification of hair cell progenitors and intermitotic migration of their nuclei in the normal and regenerating avian inner ear

1994 ◽  
Vol 14 (1) ◽  
pp. 140-152 ◽  
Author(s):  
TT Tsue ◽  
DL Watling ◽  
P Weisleder ◽  
MD Coltrera ◽  
EW Rubel
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Avian Inner Ear

scholarly journals Applying genomics to the avian inner ear: Development of subtractive cDNA resources for exploring sensory function and hair cell regeneration

Genomics ◽  
2006 ◽  
Vol 87 (6) ◽  
pp. 801-808 ◽  
Author(s):  
R. David Hawkins ◽  
Cynthia A. Helms ◽  
Julia B. Winston ◽  
Mark E. Warchol ◽  
Michael Lovett
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Sensory Function ◽  
Cell Regeneration ◽  
Hair Cell Regeneration ◽  
Ear Development ◽  
Inner Ear Development ◽  
Avian Inner Ear

Triggers of hair cell regeneration in the avian inner ear

1997 ◽  
Vol 24 (3) ◽  
pp. 221-225 ◽  
Author(s):  
Hideo Yamane ◽  
Takayuki Nakagawa ◽  
Hiroyoshi Iguchi ◽  
Shigetarou Shibata ◽  
Masahiro Takayama ◽  
...  
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Cell Regeneration ◽  
Hair Cell Regeneration ◽  
Avian Inner Ear

scholarly journals The Transcriptome of Utricle Hair Cell Regeneration in the Avian Inner Ear

2014 ◽  
Vol 34 (10) ◽  
pp. 3523-3535 ◽  
Author(s):  
Y.-C. Ku ◽  
N. A. Renaud ◽  
R. A. Veile ◽  
C. Helms ◽  
C. C. J. Voelker ◽  
...  
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Cell Regeneration ◽  
Hair Cell Regeneration ◽  
Avian Inner Ear

Cell Replacement Therapy in the Inner Ear: Implications for Cochlear Implantation

2009 ◽  
Vol 19 (2) ◽  
pp. 98-107
Author(s):  
Katherine A. Belzner ◽  
Brenda M. Ryals
Keyword(s):  
Hearing Loss ◽  
Growth Factors ◽  
Animal Models ◽  
Hair Cell ◽  
Replacement Therapy ◽  
Inner Ear ◽  
Critical Role ◽  
Future Application ◽  
Cell Replacement Therapy ◽  
Cell Replacement

Abstract This article provides an overview of recent advances in inner ear cell replacement therapy. Current strategies designed both to initiate hair cell regeneration and maintain or regenerate neural cells are reviewed. Using animal models, investigators have shown that hearing loss from hair cell and neural degeneration can be reversed through molecular and genetic manipulation. Successful strategies in animal models have included the use of growth factors, stem cells, and gene therapy. One significant challenge for the use of these strategies in humans is the development of safe, efficient, and targeted drug delivery systems. Using animal models investigators have shown that cochlear implants can be used to deliver growth factors, such as neurotrophins to the inner ear. Targeting the appropriate neural elements will be important for future application of this technique in humans. Finally, the prognosis for hearing restoration through cell replacement or maintenance therapy will certainly be influenced by the underlying etiology of the hearing loss. Audiologists will play a critical role in the future determination of candidacy for these therapeutic approaches.

scholarly journals Identification of a 275-kD protein associated with the apical surfaces of sensory hair cells in the avian inner ear.

1990 ◽  
Vol 110 (4) ◽  
pp. 1055-1066 ◽  
Author(s):  
G P Richardson ◽  
S Bartolami ◽  
I J Russell
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Hair Cells ◽  
Proximal Region ◽  
Basilar Papilla ◽  
Relative Molecular Mass ◽  
Apical Surface ◽  
Sensory Hair ◽  
Sensory Hair Cells ◽  
Avian Inner Ear

Immunological techniques have been used to generate both polyclonal and monoclonal antibodies specific for the apical ends of sensory hair cells in the avian inner ear. The hair cell antigen recognized by these antibodies is soluble in nonionic detergent, behaves on sucrose gradients primarily as a 16S particle, and, after immunoprecipitation, migrates as a polypeptide with a relative molecular mass of 275 kD on 5% SDS gels under reducing conditions. The antigen can be detected with scanning immunoelectron microscopy on the apical surface of the cell and on the stereocilia bundle but not on the kinocilium. Double label studies indicate that the entire stereocilia bundle is stained in the lagena macula (a vestibular organ), whereas in the basilar papilla (an auditory organ) only the proximal region of the stereocilia bundle nearest to the apical surface is stained. The monoclonal anti-hair cell antibodies do not stain brain, tongue, lung, liver, heart, crop, gizzard, small intestine, skeletal muscle, feather, skin, or eye tissues but do specifically stain renal corpuscles in the kidney. Experiments using organotypic cultures of the embryonic lagena macula indicate that the antibodies cause a significant increase in the steady-state stiffness of the stereocilia bundle but do not inhibit mechanotransduction. The antibodies should provide a suitable marker and/or tool for the purification of the apical sensory membrane of the hair cell.

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