Detection of substances on a paper chromatogram

1976 ◽  
Vol 10 (9) ◽  
pp. 1278-1279
Author(s):  
L. I. Brutko ◽  
S. V. Gritsenko
Keyword(s):  
Paper Chromatogram

DETECTION OF NUCLEIC ACID COMPONENTS ON PAPER CHROMATOGRAM

1954 ◽  
Vol 41 (4) ◽  
pp. 457-462 ◽  
Author(s):  
KATASHI MAKINO ◽  
KIKUO MATSUZAKI
Keyword(s):  
Nucleic Acid ◽  
Paper Chromatogram

scholarly journals A Novel Method for the Determination of Synthetic Colors in Ice Cream Samples

2004 ◽  
Vol 87 (3) ◽  
pp. 657-663 ◽  
Author(s):  
Meenakshi Tripathi ◽  
Subhash K Khanna ◽  
Mukul Das
Keyword(s):  
Indigo Carmine ◽  
Ice Cream ◽  
Trisodium Citrate ◽  
Ammonia Solution ◽  
Tween 20 ◽  
Sunset Yellow ◽  
Simple Method ◽  
Paper Chromatogram ◽  
Triton X

Abstract A simple method has been developed for the extraction, separation, and determination of synthetic colors in ice cream samples. The process involves the breakdown of emulsion by neutral detergents (Triton X-100 and Tween 20) followed by extraction with petroleum ether for removal of fat. The aqueous colored solution obtained is treated with 5% acetic acid, and the uptake of color is carried out by a wool-dyeing technique. The color is eluted from the wool with 5% ammonia solution, the solution is evaporated to dryness, and the residue is dissolved in 60% ethanol for paper chromatography using trisodium citrate–ammonia–water (2 + 5 + 95, w/v/v) as the mobile phase. The colored spots from the paper chromatogram are cut and eluted with 60% ethanol, and the absorbance is measured at the respective λ maximum corresponding to the Rf value of the appropriate standard. The recoveries of 6 colors, including sunset yellow FCF (SSYFCF), tartrazine, carmoisine, ponceau 4R, brilliant blue FCF (BBFCF), and fast green FCF from spiked samples with either detergent were found to be >90%. However, recoveries of erythrosine were 21 and 65% with Triton X-100 and Tween 20, respectively. Indigo carmine could not be recovered at all because of its fugitive property in 5% ammonia solution, which is used to strip the color from the wool. The sensitivity of the method with the use of Tween 20 is 1 ppm (1 μg/g) for the colors in spiked ice cream samples. With this method, we analyzed samples of 20 branded colored ice cream. The results showed the presence of tartrazine (8.4–43.3 ppm), SSYFCF (23.5–117.6 ppm), carmoisine (traces–53.2 ppm), erythrosine (3.5 ppm), and BBFCF (4.1 ppm) in the ice cream samples. Apart from 2 samples of tuttifruity, all of the ice cream samples showed the presence of permitted synthetic colors below the permissible level of 100 ppm established by the Prevention of Food Adulteration Act of India.

Separation and Identification of Methylated Sugars on the Paper Chromatogram

Nature ◽  
1948 ◽  
Vol 161 (4097) ◽  
pp. 720-720 ◽  
Author(s):  
F. BROWN ◽  
E. L. HIRST ◽  
L. HOUGH ◽  
J. K. N. JONES ◽  
H. WADMAN
Keyword(s):  
Paper Chromatogram

Classification of fungal antibiotics from the salting-out paper chromatogram

1961 ◽  
Vol 6 (4) ◽  
pp. 277-278 ◽  
Author(s):  
P. Nemec ◽  
V. Betina ◽  
Ludmila Kovačičová
Keyword(s):  
Salting Out ◽  
Paper Chromatogram

Biochemistry of fluoroacetate poisoning. The isolation and some properties of the fluorotricarboxylic acid inhibitor of citrate metabolism

1953 ◽  
Vol 140 (901) ◽  
pp. 497-506 ◽  
Keyword(s):  
Citric Acid ◽  
Tricarboxylic Acid Cycle ◽  
Fluorine Content ◽  
Ion Exchange Resin ◽  
Final Decision ◽  
Colour Reaction ◽  
Paper Chromatogram ◽  
Acid Inhibitor ◽  
Citrate Metabolism ◽  
Great Potency

It has been suggested that the toxicity of fluoroacetate is due to the enzymic synthesis of a fluorotricarboxylic acid, which ‘jams’ the tricarboxylic acid cycle at the citrate stage. This communication presents the proof of this hypothesis. The inhibitory substance for citrate metabolism synthesized by enzymic action from fluoroacetate has been isolated as a compound in crystalline form of great potency. Under the conditions of test it inhibits the disappearance of approximately 300 times its weight of citric acid in 30 min. The final isolation involved a separation from citric acid by the use of ion-exchange resin, and fractional extraction with ether. It is a monofluorotricarboxylic acid, as shown by its migration on a paper chromatogram, by its fluorine content (estimated spectrochemically), and by its titration curve. It does not give the colour reaction with sodium sulphide for pentabromacetone produced from citric acid by the usual methods. It gives an infra-red band which may be expected from a C-F bond. By a process of exclusion, it is considered to be a fluorocitric acid; a final decision must await synthesis.

Separation of Carboxylate Ions on the Paper Chromatogram

Nature ◽  
1950 ◽  
Vol 166 (4210) ◽  
pp. 66-67 ◽  
Author(s):  
F. BROWN ◽  
L. P. HALL
Keyword(s):  
Paper Chromatogram

scholarly journals Identification of guanosine diphosphate derivatives of d-xylose, d-glucose and d-galactose in mature strawberry leaves

1967 ◽  
Vol 105 (2) ◽  
pp. 723-728 ◽  
Author(s):  
R. R. Selvendran ◽  
F. A. Isherwood
Keyword(s):  
Isotope Dilution Method ◽  
Dilution Method ◽  
Mild Acid Hydrolysis ◽  
Paper Chromatogram ◽  
Guanosine Diphosphate ◽  
Preliminary Separation ◽  
Layer Chromatography ◽  
Derivatives Of ◽  
Dowex 1 ◽  
Mild Acid

The nucleotides from a trichloroacetic acid extract of mature strawberry leaves were separated into ten main fractions by chromatography on a Dowex 1 (formate form) column with ammonium formate as the eluting agent. One of these fractions, which was suspected to contain not only ADP but also GDP-sugars, was separated into a number of subfractions by further chromatography on a Dowex 1 (formate form) column with the formic acid system as the eluting agent. One of these subfractions was identified from its ultraviolet spectra, from its position on the two ion-exchange columns and by thin-layer chromatography as a GDP-sugar. Mild acid hydrolysis gave GDP and a mixture of sugars. The sugars, after a preliminary separation on a paper chromatogram, were identified by an isotope-dilution method. The sugars were condensed with sodium [14C]cyanide, the [14C]nitriles were hydrolysed and one of the epimeric acids was isolated, either as lactone or amide, by co-crystallization with a non-radioactive carrier. This method distinguishes between enantiomorphic sugars. d-Mannose, d-xylose, d-glucose and d-galactose were present in the proportions 40:10:1:1 respectively. The total amount of the GDP-sugars was approx. 0·1μmole/100g. of fresh leaves.

The sulpholipids of plants I. The distribution of sulpholipids and phospholipids among the major plant classes

1963 ◽  
Vol 43 (3) ◽  
pp. 605-612 ◽  
Author(s):  
Rosemary Collier ◽  
G. Y. Kennedy
Keyword(s):  
Paper Chromatography ◽  
Red Algae ◽  
Brown Algae ◽  
Broad Bean ◽  
Sphagnum Moss ◽  
Plant Tissues ◽  
Paper Chromatogram ◽  
Single Spot ◽  
Acetone Insoluble

It is shown that sulpholipid is the predominant acetone-insoluble lipid of grass, sphagnum moss, Selaginella, and of some blue-green, green and brown algae.This sulpholipid is separable into three entities by paper chromatography.Phospholipid predominates over sulpholipid in red algae, fungi and the fluffy pericarp of the broad bean.The sulpholipid in these plant tissues gives a single spot on paper chromatogramA relationship between sulpholipids and photosynthesis is suggested.

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