Viral immunoblotting: A sensitive method for detecting viral-specific oliogoclonal bands in unconcentrated cerebrospinal fluid

1984 ◽  
Vol 4 (6) ◽  
pp. 505-510 ◽  
Author(s):  
S. Moyle ◽  
G. Keir ◽  
E. J. Thompson
Keyword(s):  
Cerebrospinal Fluid ◽  
Viral Antigen ◽  
New Method ◽  
Viral Antibodies ◽  
Highly Sensitive ◽  
Immunoprecipitation Reaction ◽  
Sensitive Method

A new method for detecting viral antibodies in cerebrospinal fluid is described. The technique has many advantages over previously published methods in that it is highly sensitive eliminating the need to concentrate the CSF, takes 5 h to complete, avoids the use of radionucleides, and most importantly circumvents problems associated with prozone effects which occur in immunoprecipitation reaction since the viral antigen is immobilized on nitrocellulose membranes.

Highly sensitive method for the quantitation of homovanillic acid in cerebrospinal fluid

1980 ◽  
Vol 199 ◽  
pp. 191-197 ◽  
Author(s):  
Wolfgang Vogt ◽  
Karl Jacob ◽  
Anne-Barb Ohnesorge ◽  
Gudrun Schwertfeger
Keyword(s):  
Cerebrospinal Fluid ◽  
Homovanillic Acid ◽  
Highly Sensitive ◽  
Sensitive Method

scholarly journals A new, highly sensitive method for the detection and quantification of penicillin-binding proteins

1993 ◽  
Vol 291 (1) ◽  
pp. 19-21 ◽  
Author(s):  
M Galleni ◽  
B Lakaye ◽  
S Lepage ◽  
M Jamin ◽  
I Thamm ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Binding Proteins ◽  
New Method ◽  
Bacterial Cells ◽  
Penicillin Binding Proteins ◽  
Naked Eye ◽  
Highly Sensitive ◽  
Direct Labelling ◽  
Sensitive Method ◽  
Detection And Quantification

A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.

XII.—On a New Method of Measurement of Minute Alternating Currents

1931 ◽  
Vol 50 ◽  
pp. 166-174 ◽  
Author(s):  
D. F. Martyn
Keyword(s):  
Magnetic Field ◽  
Alternating Current ◽  
New Method ◽  
Electro Magnetic Field ◽  
Highly Sensitive ◽  
Method Of Measurement ◽  
Electro Magnetic ◽  
Sensitive Method ◽  
Plate Current

SummaryA highly sensitive method of measuring alternating currents of the order of microamperes and of any frequency is described. The current to be measured is passed through the filament of a diode valve. The main heating current of this valve is supplied by an independent oscillator working at the frequency of the current under measurement. The change in the plate current of the valve is measured, thus indicating the amplitude of the alternating current being measured. Theoretical and practical details of the methods of working are given and particulars of the method by which the calibration was tested. It is suggested that the method is particularly applicable to the determination of electro-magnetic field strengths by measuring the currents induced in an aerial.

scholarly journals Increased cerebrospinal fluid adenosine 5'-triphosphate in patients with amyotrophic lateral sclerosis

BMC Neurology ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Takamasa Nukui ◽  
Atsushi Matsui ◽  
Hideki Niimi ◽  
Tomoyuki Sugimoto ◽  
Tomohiro Hayashi ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Cerebrospinal Fluid ◽  
Serum Creatinine ◽  
Disease Severity ◽  
Assay System ◽  
Atp Levels ◽  
Sum Score ◽  
Highly Sensitive ◽  
Severe Group ◽  
Lateral Sclerosis

Abstract Background Extracellular adenosine 5'-triphosphate (ATP) has been suggested to cause neuroinflammation and motor neuron degeneration by activating microglia and astrocytes in amyotrophic lateral sclerosis (ALS). Since we have developed a highly sensitive ATP assay system, we examined cerebrospinal fluid (CSF) ATP levels in patients with ALS whether it can be a useful biomarker in ALS. Methods Forty-eight CSF samples from 44 patients with ALS were assayed for ATP with a newly established, highly sensitive assay system using luciferase luminous reaction. CSF samples from patients with idiopathic normal pressure hydrocephalus (iNPH) were assayed as a control. Patients were divided into two groups depending on their disease severity, as evaluated using the Medical Research Council (MRC) sum score. Correlations between the CSF ATP levels and other factors, including clinical data and serum creatinine levels, were evaluated. Results CSF ATP levels were significantly higher in patients with ALS than in the iNPH (716 ± 411 vs. 3635 ± 5465 pmol/L, p < 0.01). CSF ATP levels were significantly higher in the more severe group than in the iNPH group (6860 ± 8312 vs. 716 ± 411 pmol/L, p < 0.05) and mild group (6860 ± 8312 vs. 2676 ± 3959 pmol/L, p < 0.05) respectively. ALS functional rating scale-revised (ALSFRS-R) (37.9 ± 5.7 vs. 42.4 ± 2.8, p < 0.01) and serum creatinine levels (0.51 ± 0.13 vs. 0.68 ± 0.23 mg/dL, p < 0.05) were significantly lower in the severe group than in the mild group respectively. A negative correlation of CSF ATP levels with MRC sum score was demonstrated in the correlation analysis adjusted for age and sex (r = -0.3, p = 0.08). Conclusions Extracellular ATP is particularly increased in the CSF of patients with advanced ALS. CSF ATP levels may be a useful biomarker for evaluating disease severity in patients with ALS.

Smartphone Based Highly Sensitive Visualized Detection of Acid Phosphatase Enzyme Activity

2021 ◽  
Author(s):  
Rui Li ◽  
Yanan Sun ◽  
Lihua Jin ◽  
Xiaohong Qiao ◽  
Cong Li ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Point Of Care ◽  
Rapid Development ◽  
Practical Application ◽  
Fast Analysis ◽  
Highly Sensitive ◽  
Visualized Detection ◽  
Sensitive Method ◽  
Phosphatase Enzyme

With the rapid development of point-of-care (POC) technologies, the improvement of sensitive method featured with fast analysis and affordable devices has become an emerging requirement for the practical application. In...

scholarly journals A New Ultrasensitive Bioluminescence-Based Method for Assaying Monoacylglycerol Lipase

2021 ◽  
Vol 22 (11) ◽  
pp. 6148
Author(s):  
Matteo Miceli ◽  
Silvana Casati ◽  
Pietro Allevi ◽  
Silvia Berra ◽  
Roberta Ottria ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Kinetic Constants ◽  
New Method ◽  
Monoacylglycerol Lipase ◽  
Enzymatic Cleavage ◽  
Highly Sensitive ◽  
Bioluminescence Assay ◽  
Identification And Characterization ◽  
In Cells

A novel bioluminescent Monoacylglycerol lipase (MAGL) substrate 6-O-arachidonoylluciferin, a D-luciferin derivative, was synthesized, physico-chemically characterized, and used as highly sensitive substrate for MAGL in an assay developed for this purpose. We present here a new method based on the enzymatic cleavage of arachidonic acid with luciferin release using human Monoacylglycerol lipase (hMAGL) followed by its reaction with a chimeric luciferase, PLG2, to produce bioluminescence. Enzymatic cleavage of the new substrate by MAGL was demonstrated, and kinetic constants Km and Vmax were determined. 6-O-arachidonoylluciferin has proved to be a highly sensitive substrate for MAGL. The bioluminescence assay (LOD 90 pM, LOQ 300 pM) is much more sensitive and should suffer fewer biological interferences in cells lysate applications than typical fluorometric methods. The assay was validated for the identification and characterization of MAGL modulators using the well-known MAGL inhibitor JZL184. The use of PLG2 displaying distinct bioluminescence color and kinetics may offer a highly desirable opportunity to extend the range of applications to cell-based assays.

Highly sensitive method for concentration and absorption measurements by guided-wave resonance

2000 ◽  
Author(s):  
Guang Chen ◽  
Yi Jiang ◽  
Zhuangqi Cao ◽  
Qishun Shen ◽  
Xiaoming Dou ◽  
...  
Keyword(s):  
Guided Wave ◽  
Wave Resonance ◽  
Highly Sensitive ◽  
Sensitive Method ◽  
Absorption Measurements

The relationship between CSF and serum findings when diagnosed with syphilis

1935 ◽  
Vol 31 (1) ◽  
pp. 138-138
Author(s):  
F. Каug
Keyword(s):  
Cerebrospinal Fluid ◽  
Blood Serum ◽  
Specific Reaction ◽  
Highly Sensitive ◽  
Positive Serum ◽  
The Relationship

The authors studied the question of whether a highly sensitive and strictly specific reaction of blood serum flocculation can be an auxiliary tool in assessing positive reactions of cerebrospinal fluid. A study of 1400 cases has proven that a positive serum flocculation reaction invariably accompanies a special colloidal reaction (Goldsolreaktion).

Highly sensitive method for aldehydes detection: Application to furfurals analysis in raisin and bovine milk powder

2017 ◽  
Vol 987 ◽  
pp. 47-55 ◽  
Author(s):  
Yao Sun ◽  
Zhaobing Guan ◽  
Hongwei Cai ◽  
Yiyong Huang ◽  
Yawei Lin ◽  
...  
Keyword(s):  
Bovine Milk ◽  
Milk Powder ◽  
Highly Sensitive ◽  
Sensitive Method
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